Protein profile

KP13_03230

Nitrogen regulatory protein

Genome: KpKP13

Gene: ptsN AHE42471.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GZ03
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Amino acids 162
Annotations 5
Features 16
PDB binders 0
Druggability 0.976

Overview

Basic information about this protein and its source genome.

Accession
KP13_03230
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
ptsN AHE42471.1
Status
annotated
Amino acids
162
Structure source
AlphaFold + ColabFold
GO
GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0030295 Binds to and increases the activity of a protein kinase, an enzyme which phosphorylates a protein.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
91.411
DEG E-value
3.23e-108
Localization
Cytoplasmic
ColabFold pLDDT
91.26

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.976
Structure A0A0H3GZ03
Pocket Pocket 1
P2Rank 0.041
Structure A0A0H3GZ03
Pocket Pocket 1
ColabFold model
FPocket 0.985 · Pocket 1
P2Rank 0.119 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 127 / 4744 genomes with a hit
Normalized 0.027

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0030295 Binds to and increases the activity of a protein kinase, an enzyme which phosphorylates a protein.

Sequence Features

Domain/signature hits from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
13 155 Pfam PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
13 155 InterPro IPR002178 PTS EIIA type-2 domain
12 156 ProSiteProfiles PS51094 PTS_EIIA type-2 domain profile.
12 156 InterPro IPR002178 PTS EIIA type-2 domain
9 157 PANTHER PTHR47738 PTS SYSTEM FRUCTOSE-LIKE EIIA COMPONENT-RELATED
9 155 NCBIfam TIGR01419 PTS IIA-like nitrogen regulatory protein PtsN
9 155 InterPro IPR006320 Phosphotransferase system, IIA-like nitrogen-regulatory protein PtsN
9 155 SUPERFAMILY SSF55804 Phoshotransferase/anion transport protein
9 155 InterPro IPR016152 Phosphotransferase/anion transporter
14 153 CDD cd00211 PTS_IIA_fru
14 153 InterPro IPR002178 PTS EIIA type-2 domain
1 161 Gene3D G3DSA:3.40.930.10 -
1 161 InterPro IPR016152 Phosphotransferase/anion transporter
1 162 FunFam G3DSA:3.40.930.10:FF:000003 PTS IIA-like nitrogen regulatory protein PtsN
58 74 ProSitePatterns PS00372 PTS EIIA domains phosphorylation site signature 2.
58 74 InterPro IPR002178 PTS EIIA type-2 domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GZ03
AlphaFold — — full sequence — Viewing
ColabFold KP13_03230
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.976