Protein profile

KP13_01101

Transcription elongation protein nusA

Genome: KpKP13

Gene: nusA AHE42507.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GW50
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Amino acids 495
Annotations 9
Features 54
PDB binders 0
Druggability 0.886

Overview

Basic information about this protein and its source genome.

Accession
KP13_01101
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
nusA AHE42507.1
Status
annotated
Amino acids
495
Structure source
AlphaFold + ColabFold
GO
GO:0003676 Binding to a nucleic acid. GO:0000166 Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose. GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. GO:0031564 A positive regulation of gene expression mechanism that allows RNA polymerase to continue transcription beyond a termination site, thus allowing expression of downstream genes under specific conditions. GO:0003723 Binding to an RNA molecule or a portion thereof. GO:0031554 Any process that modulates the frequency, rate, extent, or location of DNA-templated transcription termination, the process in which transcription is completed; the formation of phosphodiester bonds ceases, the RNA-DNA hybrid dissociates, and RNA polymerase releases the DNA.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
95.758
DEG E-value
0.0
Localization
Cytoplasmic
ColabFold pLDDT
87.67

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.886
Structure A0A0H3GW50
Pocket Pocket 2
P2Rank 0.034
Structure A0A0H3GW50
Pocket Pocket 1
ColabFold model
FPocket 0.924 · Pocket 7
P2Rank 0.12 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 191 / 4744 genomes with a hit
Normalized 0.04

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Gene Ontology (GO)

9
  • GO:0003676 Binding to a nucleic acid.
  • GO:0000166 Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
  • GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
  • GO:0031564 A positive regulation of gene expression mechanism that allows RNA polymerase to continue transcription beyond a termination site, thus allowing expression of downstream genes under specific conditions.
  • GO:0003723 Binding to an RNA molecule or a portion thereof.
  • GO:0031554 Any process that modulates the frequency, rate, extent, or location of DNA-templated transcription termination, the process in which transcription is completed; the formation of phosphodiester bonds ceases, the RNA-DNA hybrid dissociates, and RNA polymerase releases the DNA.
  • GO:0006353 The completion of transcription: the RNA polymerase pauses, the RNA-DNA hybrid dissociates, followed by the release of the RNA polymerase from its DNA template.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0003746 Functions in chain elongation during polypeptide synthesis at the ribosome.

Sequence Features

Domain/signature hits from InterPro and related databases.

54 records
Show feature table
Start End DB Term Name
230 297 Pfam PF13184 NusA-like KH domain
230 297 InterPro IPR025249 KH domain, NusA-like
279 343 SUPERFAMILY SSF54814 Prokaryotic type KH domain (KH-domain type II)
279 343 InterPro IPR009019 K homology domain superfamily, prokaryotic type
278 346 FunFam G3DSA:3.30.300.20:FF:000005 Transcription termination/antitermination protein NusA
1 387 PANTHER PTHR22648 TRANSCRIPTION TERMINATION FACTOR NUSA
1 387 InterPro IPR030842 Transcription termination/antitermination protein NusA, bacterial
278 343 Gene3D G3DSA:3.30.300.20 -
278 343 InterPro IPR015946 K homology domain-like, alpha/beta
135 198 Gene3D G3DSA:2.40.50.140 -
135 198 InterPro IPR012340 Nucleic acid-binding, OB-fold
431 490 FunFam G3DSA:1.10.150.20:FF:000015 Transcription termination/antitermination protein NusA
202 276 CDD cd02134 KH-II_NusA_rpt1
2 410 Hamap MF_00945_B Transcription termination/antitermination protein NusA [nusA].
2 410 InterPro IPR030842 Transcription termination/antitermination protein NusA, bacterial
352 421 Gene3D G3DSA:1.10.150.20 -
365 414 NCBIfam TIGR01954 transcription termination factor NusA, C-terminal duplication
365 414 InterPro IPR010214 Transcription termination factor NusA, C-terminal duplication
440 489 NCBIfam TIGR01954 transcription termination factor NusA, C-terminal duplication
440 489 InterPro IPR010214 Transcription termination factor NusA, C-terminal duplication
302 338 ProSiteProfiles PS50084 Type-1 KH domain profile.
1 129 FunFam G3DSA:3.30.1480.10:FF:000001 Transcription termination/antitermination protein NusA
4 342 NCBIfam TIGR01953 transcription termination factor NusA
4 342 InterPro IPR010213 Transcription termination factor NusA
432 486 Pfam PF14520 Helix-hairpin-helix domain
4 123 Pfam PF08529 NusA N-terminal domain
4 123 InterPro IPR013735 Transcription factor NusA, N-terminal
431 490 Gene3D G3DSA:1.10.150.20 -
133 200 SMART SM00316 S1_6
133 200 InterPro IPR022967 RNA-binding domain, S1
135 200 ProSiteProfiles PS50126 S1 domain profile.
135 200 InterPro IPR003029 S1 domain
135 198 FunFam G3DSA:2.40.50.140:FF:000092 Transcription termination/antitermination protein NusA
403 423 Coils Coil Coil
1 126 SUPERFAMILY SSF69705 Transcription factor NusA, N-terminal domain
1 126 InterPro IPR036555 NusA, N-terminal domain superfamily
199 277 FunFam G3DSA:3.30.300.20:FF:000002 Transcription termination/antitermination protein NusA
429 492 SUPERFAMILY SSF47794 Rad51 N-terminal domain-like
429 492 InterPro IPR010995 DNA repair Rad51/transcription factor NusA, alpha-helical
352 421 FunFam G3DSA:1.10.150.20:FF:000018 Transcription termination/antitermination protein NusA
132 199 CDD cd04455 S1_NusA
201 278 SUPERFAMILY SSF54814 Prokaryotic type KH domain (KH-domain type II)
201 278 InterPro IPR009019 K homology domain superfamily, prokaryotic type
134 195 Pfam PF00575 S1 RNA binding domain
134 195 InterPro IPR003029 S1 domain
1 129 Gene3D G3DSA:3.30.1480.10 -
1 129 InterPro IPR036555 NusA, N-terminal domain superfamily
355 415 SUPERFAMILY SSF47794 Rad51 N-terminal domain-like
355 415 InterPro IPR010995 DNA repair Rad51/transcription factor NusA, alpha-helical
279 339 CDD cd22529 KH-II_NusA_rpt2
132 199 SUPERFAMILY SSF50249 Nucleic acid-binding proteins
132 199 InterPro IPR012340 Nucleic acid-binding, OB-fold
199 277 Gene3D G3DSA:3.30.300.20 -
199 277 InterPro IPR015946 K homology domain-like, alpha/beta

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GW50
AlphaFold — — full sequence — Viewing
ColabFold KP13_01101
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.886

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.2 0.011