Protein profile

KP13_02864

Ribosomal RNA large subunit methyltransferase G

Genome: KpKP13

Gene: AHE42567.1 rlmG Structure source: AlphaFold + ColabFold UniProt A0A0H3GXW1
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Amino acids 376
Annotations 9
Features 18
PDB binders 0
Druggability 0.765

Overview

Basic information about this protein and its source genome.

Accession
KP13_02864
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE42567.1 rlmG
Status
annotated
Amino acids
376
Structure source
AlphaFold + ColabFold
EC
2.1.1.174
GO
GO:0031167 The posttranscriptional addition of methyl groups to specific residues in an rRNA molecule. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0003676 Binding to a nucleic acid. GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule. GO:0008990 Catalysis of the reaction: S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N2-methylguanine. GO:0032259 The process in which a methyl group is covalently attached to a molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
94.92

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.765
Structure A0A0H3GXW1
Pocket Pocket 13
P2Rank 0.937
Structure A0A0H3GXW1
Pocket Pocket 1
ColabFold model
FPocket 0.91 · Pocket 1
P2Rank 0.95 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 101 / 4744 genomes with a hit
Normalized 0.021

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0031167 The posttranscriptional addition of methyl groups to specific residues in an rRNA molecule.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0003676 Binding to a nucleic acid.
  • GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule.
  • GO:0008990 Catalysis of the reaction: S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N2-methylguanine.
  • GO:0032259 The process in which a methyl group is covalently attached to a molecule.
  • GO:0008757 Catalysis of the transfer of a methyl group from S-adenosyl-L-methionine to a substrate.
  • GO:0052916 Catalysis of the reaction: S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = N(2)-methylguanosine(1835) in 23S rRNA + S-adenosyl-L-homocysteine.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records
Show feature table
Start End DB Term Name
1 372 PIRSF PIRSF037565 RRNA_m2G_Mtase_RsmD
1 372 InterPro IPR017237 rRNA (guanine-N2-)-methyltransferase, RlmG
300 306 ProSitePatterns PS00092 N-6 Adenine-specific DNA methylases signature.
300 306 InterPro IPR002052 DNA methylase, N-6 adenine-specific, conserved site
181 376 Gene3D G3DSA:3.40.50.150 Vaccinia Virus protein VP39
181 376 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
230 337 CDD cd02440 AdoMet_MTases
181 375 FunFam G3DSA:3.40.50.150:FF:000046 Ribosomal RNA large subunit methyltransferase G
197 368 Pfam PF05175 Methyltransferase small domain
197 368 InterPro IPR007848 Methyltransferase small domain
28 371 PANTHER PTHR47816 RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE C
28 371 InterPro IPR046977 rRNA (guanine-N(2)-)-methyltransferase RsmC/RlmG
9 179 Gene3D G3DSA:3.40.50.150 Vaccinia Virus protein VP39
9 179 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
191 369 SUPERFAMILY SSF53335 S-adenosyl-L-methionine-dependent methyltransferases
191 369 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
1 371 Hamap MF_01859 Ribosomal RNA large subunit methyltransferase G [rlmG].
1 371 InterPro IPR017237 rRNA (guanine-N2-)-methyltransferase, RlmG

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GXW1
AlphaFold — — full sequence — Viewing
ColabFold KP13_02864
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
13 0.765

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 16.64 0.783
2 3.55 0.131
3 3.5 0.128
4 2.14 0.05