Protein profile

KP13_02827

G/U mismatch-specific DNA glycosylase

Genome: KpKP13

Gene: mug AHE42604.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GTH2
Amino acids 168
Annotations 7
Features 15
PDB binders 2
Druggability 0.868

Overview

Basic information about this protein and its source genome.

Accession
KP13_02827
Gene
mug AHE42604.1
Status
annotated
Amino acids
168
Structure source
AlphaFold + ColabFold
GO
GO:0006285 The formation of an AP site, a deoxyribose sugar with a missing base, by DNA glycosylase which recognizes an altered base in DNA and catalyzes its hydrolytic removal. This sugar phosphate is the substrate recognized by the AP endonuclease, which cuts the DNA phosphodiester backbone at the 5' side of the altered site to leave a gap which is subsequently repaired. GO:0000700 Catalysis of the removal of single bases present in mismatches by the cleavage the N-C1' glycosidic bond between the target damaged DNA base and the deoxyribose sugar. The reaction releases a free base and leaves an apurinic/apyrimidinic (AP) site. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). GO:0008263 Catalysis of the removal of mismatched pyrimidine bases in DNA. Enzymes with this activity recognize and remove pyrimidines present in mismatches by cleaving the N-C1' glycosidic bond between the target damaged DNA base and the deoxyribose sugar. The reaction releases a free base and leaves an apyrimidinic (AP) site. GO:0004844 Catalysis of the cleavage of the N-C1' glycosidic bond between the damaged DNA base and the deoxyribose sugar, releasing a free base and leaving an apyrimidinic (AP) site. Enzymes with this activity recognize and remove uracil bases in DNA that result from the deamination of cytosine or the misincorporation of dUTP opposite an adenine.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
47.5
Human E-value
6.66e-07
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Unknown
ColabFold pLDDT
96.06

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.868
Structure A0A0H3GTH2
Pocket Pocket 1
P2Rank 0.819
Structure A0A0H3GTH2
Pocket Pocket 1
ColabFold model
FPocket 0.836 · Pocket 9
P2Rank 0.825 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 80 / 4744 genomes with a hit
Normalized 0.017

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0006285 The formation of an AP site, a deoxyribose sugar with a missing base, by DNA glycosylase which recognizes an altered base in DNA and catalyzes its hydrolytic removal. This sugar phosphate is the substrate recognized by the AP endonuclease, which cuts the DNA phosphodiester backbone at the 5' side of the altered site to leave a gap which is subsequently repaired.
  • GO:0000700 Catalysis of the removal of single bases present in mismatches by the cleavage the N-C1' glycosidic bond between the target damaged DNA base and the deoxyribose sugar. The reaction releases a free base and leaves an apurinic/apyrimidinic (AP) site.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0008263 Catalysis of the removal of mismatched pyrimidine bases in DNA. Enzymes with this activity recognize and remove pyrimidines present in mismatches by cleaving the N-C1' glycosidic bond between the target damaged DNA base and the deoxyribose sugar. The reaction releases a free base and leaves an apyrimidinic (AP) site.
  • GO:0004844 Catalysis of the cleavage of the N-C1' glycosidic bond between the damaged DNA base and the deoxyribose sugar, releasing a free base and leaving an apyrimidinic (AP) site. Enzymes with this activity recognize and remove uracil bases in DNA that result from the deamination of cytosine or the misincorporation of dUTP opposite an adenine.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
3 159 SMART SM00986 UDG_2
3 159 InterPro IPR005122 Uracil-DNA glycosylase-like
3 159 SMART SM00987 UDG_2_a
2 162 PANTHER PTHR12159 G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE
2 162 InterPro IPR015637 Uracil DNA glycosylase family 2
1 168 Hamap MF_01956 G/U mismatch-specific DNA glycosylase [mug].
1 168 InterPro IPR023502 G/U mismatch-specific DNA glycosylase MUG, bacterial
1 168 Gene3D G3DSA:3.40.470.10 -
1 168 InterPro IPR036895 Uracil-DNA glycosylase-like domain superfamily
6 159 Pfam PF03167 Uracil DNA glycosylase superfamily
6 159 InterPro IPR005122 Uracil-DNA glycosylase-like
2 159 CDD cd10028 UDG-F2_TDG_MUG
2 159 InterPro IPR015637 Uracil DNA glycosylase family 2
2 161 SUPERFAMILY SSF52141 Uracil-DNA glycosylase-like
2 161 InterPro IPR036895 Uracil-DNA glycosylase-like domain superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GTH2
AlphaFold full sequence Viewing
ColabFold KP13_02827
ColabFold full sequence Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.868

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 11.09 0.595

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

16 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
1RT Q13569 155.1 Da LogP -0.95 TPSA 109.1 ✓ Ro5 ✓ Clean C1=C(C(=NC(=O)N1)N)C(=O)O
HMU Q13569 142.1 Da LogP -1.44 TPSA 86.0 ✓ Ro5 ✓ Clean C1=C(C(=O)NC(=O)N1)CO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.