Protein profile

KP13_02805

Glutamate-ammonia-ligase adenylyltransferase

Genome: KpKP13

Gene: glnE AHE42625.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3H378
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Amino acids 945
Annotations 2
Features 34
PDB binders 0
Druggability 0.458

Overview

Basic information about this protein and its source genome.

Accession
KP13_02805
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
glnE AHE42625.1
Status
annotated
Amino acids
945
Structure source
AlphaFold + ColabFold
GO
GO:0016779 Catalysis of the transfer of a nucleotidyl group from one compound (donor) to another (acceptor). GO:0008882 Catalysis of the reaction: ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)].

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
92.99

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.458
Structure A0A0H3H378
Pocket Pocket 7
P2Rank 0.67
Structure A0A0H3H378
Pocket Pocket 1
ColabFold model
FPocket 0.264 · Pocket 54
P2Rank 0.666 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 142 / 4744 genomes with a hit
Normalized 0.03

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 GO

Gene Ontology (GO)

2
  • GO:0016779 Catalysis of the transfer of a nucleotidyl group from one compound (donor) to another (acceptor).
  • GO:0008882 Catalysis of the reaction: ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)].

Sequence Features

Domain/signature hits from InterPro and related databases.

34 records
Show feature table
Start End DB Term Name
619 810 CDD cd05401 NT_GlnE_GlnD_like
93 282 CDD cd05401 NT_GlnE_GlnD_like
812 945 Gene3D G3DSA:1.20.120.330 Nucleotidyltransferases domain 2
812 945 FunFam G3DSA:1.20.120.330:FF:000008 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
285 439 FunFam G3DSA:1.20.120.330:FF:000005 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
297 436 Pfam PF08335 GlnD PII-uridylyltransferase
297 436 InterPro IPR013546 PII-uridylyltransferase/Glutamine-synthetase adenylyltransferase
825 917 Pfam PF08335 GlnD PII-uridylyltransferase
825 917 InterPro IPR013546 PII-uridylyltransferase/Glutamine-synthetase adenylyltransferase
287 436 SUPERFAMILY SSF81593 Nucleotidyltransferase substrate binding subunit/domain
1 110 FunFam G3DSA:1.10.4050.10:FF:000001 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
27 945 Hamap MF_00802 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme [glnE].
27 945 InterPro IPR023057 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
285 440 Gene3D G3DSA:1.20.120.330 Nucleotidyltransferases domain 2
449 561 FunFam G3DSA:1.20.120.1510:FF:000001 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
11 944 PANTHER PTHR30621 GLUTAMINE SYNTHETASE ADENYLYLTRANSFERASE
11 944 InterPro IPR023057 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
111 284 FunFam G3DSA:3.30.460.10:FF:000014 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
818 943 SUPERFAMILY SSF81593 Nucleotidyltransferase substrate binding subunit/domain
525 815 SUPERFAMILY SSF81301 Nucleotidyltransferase
525 815 InterPro IPR043519 Nucleotidyltransferase superfamily
111 284 Gene3D G3DSA:3.30.460.10 Beta Polymerase, domain 2
111 284 InterPro IPR043519 Nucleotidyltransferase superfamily
1 110 Gene3D G3DSA:1.10.4050.10 Glutamine synthase adenylyltransferase GlnE
562 811 FunFam G3DSA:3.30.460.10:FF:000009 Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme
449 561 Gene3D G3DSA:1.20.120.1510 -
562 811 Gene3D G3DSA:3.30.460.10 Beta Polymerase, domain 2
562 811 InterPro IPR043519 Nucleotidyltransferase superfamily
28 286 SUPERFAMILY SSF81301 Nucleotidyltransferase
28 286 InterPro IPR043519 Nucleotidyltransferase superfamily
31 275 Pfam PF03710 Glutamate-ammonia ligase adenylyltransferase
31 275 InterPro IPR005190 Glutamate-ammonia ligase adenylyltransferase, repeated domain
553 805 Pfam PF03710 Glutamate-ammonia ligase adenylyltransferase
553 805 InterPro IPR005190 Glutamate-ammonia ligase adenylyltransferase, repeated domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3H378
AlphaFold — — full sequence — Viewing
ColabFold KP13_02805
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
7 0.458

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 9.0 0.482
2 8.88 0.476
3 4.3 0.178
4 2.86 0.09
5 2.78 0.085