Protein profile

KP13_02747

Nucleoside permease nupG

Genome: KpKP13

Gene: nupG AHE42685.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GT64
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Amino acids 417
Annotations 9
Features 55
PDB binders 0
Druggability 0.589

Overview

Basic information about this protein and its source genome.

Accession
KP13_02747
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
nupG AHE42685.1
Status
annotated
Amino acids
417
Structure source
AlphaFold + ColabFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0005337 Enables the transfer of a nucleoside, a nucleobase linked to either beta-D-ribofuranose (ribonucleoside) or 2-deoxy-beta-D-ribofuranose, (a deoxyribonucleotide) from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:1901642 The directed movement of nucleoside across a membrane. GO:0015858 The directed movement of a nucleoside, a nucleobase linked to either beta-D-ribofuranose (ribonucleoside) or 2-deoxy-beta-D-ribofuranose, (a deoxyribonucleotide), into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
43.405
DEG E-value
8.74e-112
Localization
CytoplasmicMembrane
ColabFold pLDDT
92.29

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.589
Structure A0A0H3GT64
Pocket Pocket 20
P2Rank 0.899
Structure A0A0H3GT64
Pocket Pocket 1
ColabFold model
FPocket 0.691 · Pocket 13
P2Rank 0.899 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 151 / 4744 genomes with a hit
Normalized 0.032

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Gene Ontology (GO)

9
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0005337 Enables the transfer of a nucleoside, a nucleobase linked to either beta-D-ribofuranose (ribonucleoside) or 2-deoxy-beta-D-ribofuranose, (a deoxyribonucleotide) from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:1901642 The directed movement of nucleoside across a membrane.
  • GO:0015858 The directed movement of a nucleoside, a nucleobase linked to either beta-D-ribofuranose (ribonucleoside) or 2-deoxy-beta-D-ribofuranose, (a deoxyribonucleotide), into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0015212 Enables the transfer of cytidine, cytosine riboside, from one side of a membrane to the other.
  • GO:0015506 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: nucleoside(out) + H+(out) = nucleoside(in) + H+(in).
  • GO:0015213 Enables the transfer of uridine, uracil riboside, from one side of a membrane to the other.

Sequence Features

Domain/signature hits from InterPro and related databases.

55 records
Show feature table
Start End DB Term Name
301 305 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
311 333 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
195 403 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
195 403 InterPro IPR036259 MFS transporter superfamily
5 185 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
5 185 InterPro IPR036259 MFS transporter superfamily
162 184 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
9 406 SUPERFAMILY SSF103473 MFS general substrate transporter
9 406 InterPro IPR036259 MFS transporter superfamily
32 36 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
381 402 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
209 417 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
209 417 InterPro IPR020846 Major facilitator superfamily domain
6 403 CDD cd06177 MFS_NHS
370 380 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
2 412 PANTHER PTHR23522 BLL5896 PROTEIN
380 402 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
253 275 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
180 210 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 417 Hamap MF_02049 Nucleoside permease NupG [nupG].
1 417 InterPro IPR033667 Nucleoside permease NupG
403 417 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
39 61 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
90 94 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
37 58 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
135 152 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
5 189 FunFam G3DSA:1.20.1250.20:FF:000012 Nucleoside permease NupG
282 300 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 414 NCBIfam TIGR00889 nucleoside permease
1 414 InterPro IPR004740 Nucleoside:H+ symporter
117 136 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
68 90 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
195 404 FunFam G3DSA:1.20.1250.20:FF:000015 Nucleoside permease NupG
328 347 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
348 369 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
282 301 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
156 160 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
95 116 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
236 254 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
137 155 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
161 179 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
70 89 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
255 275 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
95 114 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
306 327 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
276 281 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
211 235 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 406 Pfam PF03825 Nucleoside H+ symporter
1 406 InterPro IPR004740 Nucleoside:H+ symporter
211 233 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
346 365 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
59 69 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GT64
AlphaFold — — full sequence — Viewing
ColabFold KP13_02747
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
6 0.291
13 0.097
5 0.085
2 0.006

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 29.8 0.899
2 7.5 0.332
3 6.45 0.273
4 6.13 0.252
5 4.12 0.136