Protein profile

KP13_02746

Membrane-bound lytic murein transglycosylase C

Genome: KpKP13

Gene: AHE42686.1 mltC Structure source: AlphaFold + ColabFold UniProt A0A0W8AJT4
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Amino acids 361
Annotations 8
Features 23
PDB binders 1
Druggability 0.297

Overview

Basic information about this protein and its source genome.

Accession
KP13_02746
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE42686.1 mltC
Status
annotated
Amino acids
361
Structure source
AlphaFold + ColabFold
EC
4.2.2.n1
GO
GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures. GO:0008933 Catalysis of the cleavage of a peptidoglycan chain into a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end. Includes endolytic transglycosylase activity that fragments the glycan chain internally and exolytic transgylcosylase activity that cleaves a terminal disaccharide from the end of the glycan strand. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0016798 Catalysis of the hydrolysis of any glycosyl bond. GO:0000270 The chemical reactions and pathways involving peptidoglycans, any of a class of glycoconjugates found only in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides. GO:0016998 The chemical reactions and pathways resulting in the breakdown of macromolecules that form part of a cell wall.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Unknown
ColabFold pLDDT
89.82

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.297
Structure A0A0W8AJT4
Pocket Pocket 4
P2Rank 0.205
Structure A0A0W8AJT4
Pocket Pocket 1
ColabFold model
FPocket 0.315 · Pocket 1
P2Rank 0.277 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 138 / 4744 genomes with a hit
Normalized 0.029

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures.
  • GO:0008933 Catalysis of the cleavage of a peptidoglycan chain into a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end. Includes endolytic transglycosylase activity that fragments the glycan chain internally and exolytic transgylcosylase activity that cleaves a terminal disaccharide from the end of the glycan strand.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0016798 Catalysis of the hydrolysis of any glycosyl bond.
  • GO:0000270 The chemical reactions and pathways involving peptidoglycans, any of a class of glycoconjugates found only in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.
  • GO:0016998 The chemical reactions and pathways resulting in the breakdown of macromolecules that form part of a cell wall.
  • GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.

Sequence Features

Domain/signature hits from InterPro and related databases.

23 records
Show feature table
Start End DB Term Name
197 356 CDD cd16893 LT_MltC_MltE
1 25 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
1 20 Phobius SIGNAL_PEPTIDE Signal peptide region
1 5 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
1 25 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
32 193 Pfam PF11873 Membrane-bound lytic murein transglycosylase C, N-terminal domain
32 193 InterPro IPR024570 Murein transglycosylase-C, N-terminal
198 320 Pfam PF01464 Transglycosylase SLT domain
198 320 InterPro IPR008258 Transglycosylase SLT domain 1
5 360 PANTHER PTHR37423 SOLUBLE LYTIC MUREIN TRANSGLYCOSYLASE-RELATED
1 18 ProSiteProfiles PS51257 Prokaryotic membrane lipoprotein lipid attachment site profile.
193 359 SUPERFAMILY SSF53955 Lysozyme-like
193 359 InterPro IPR023346 Lysozyme-like domain superfamily
2 360 Hamap MF_01616 Membrane-bound lytic murein transglycosylase C [mltC].
2 360 InterPro IPR023664 Murein transglycosylase-C
6 16 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
17 20 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 21 SignalP_EUK SignalP-noTM SignalP-noTM
179 361 Gene3D G3DSA:1.10.530.10 -
21 361 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
215 243 ProSitePatterns PS00922 Prokaryotic transglycosylases signature.
215 243 InterPro IPR000189 Prokaryotic transglycosylase, active site
178 361 FunFam G3DSA:1.10.530.10:FF:000002 Membrane-bound lytic murein transglycosylase C

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0W8AJT4
AlphaFold — — full sequence — Viewing
ColabFold KP13_02746
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.297

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 3.16 0.107
2 1.51 0.022

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

2 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
BLG
1SLY
P0AGC3 552.6 Da LogP -6.16 TPSA 271.9 3 viol. ✓ Clean CC(=O)N[C@@H]1[C@H]([C@@H]([C@H](O[C@H]1O[C@H]2…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.