Protein profile

KP13_02743

tRNA (guanine-N(7)-)-methyltransferase

Genome: KpKP13

Gene: trmB AHE42689.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GT61
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Amino acids 239
Annotations 4
Features 14
PDB binders 0
Druggability 0.757

Overview

Basic information about this protein and its source genome.

Accession
KP13_02743
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
trmB AHE42689.1
Status
annotated
Amino acids
239
Structure source
AlphaFold + ColabFold
EC
2.1.1.33
GO
GO:0008176 Catalysis of the reaction: guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteine. GO:0006400 The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically. GO:0043527 A multimeric protein complex involved in the methylation of specific nucleotides in tRNA.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
27.119
Human E-value
6.87e-06
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
67.932
DEG E-value
2.4e-121
Localization
Cytoplasmic
ColabFold pLDDT
94.62

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.757
Structure A0A0H3GT61
Pocket Pocket 14
P2Rank 0.717
Structure A0A0H3GT61
Pocket Pocket 1
ColabFold model
FPocket 0.878 · Pocket 14
P2Rank 0.635 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 197 / 4744 genomes with a hit
Normalized 0.042

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 3 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

3
  • GO:0008176 Catalysis of the reaction: guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteine.
  • GO:0006400 The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically.
  • GO:0043527 A multimeric protein complex involved in the methylation of specific nucleotides in tRNA.

Sequence Features

Domain/signature hits from InterPro and related databases.

14 records
Show feature table
Start End DB Term Name
30 239 FunFam G3DSA:3.40.50.150:FF:000024 tRNA (guanine-N(7)-)-methyltransferase
18 237 SUPERFAMILY SSF53335 S-adenosyl-L-methionine-dependent methyltransferases
18 237 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
30 239 Gene3D G3DSA:3.40.50.150 Vaccinia Virus protein VP39
30 239 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
24 238 Hamap MF_01057 tRNA (guanine-N(7)-)-methyltransferase [trmB].
24 238 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type
50 238 NCBIfam TIGR00091 tRNA (guanosine(46)-N7)-methyltransferase TrmB
50 238 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type
64 233 Pfam PF02390 Putative methyltransferase
64 233 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type
21 237 ProSiteProfiles PS51625 SAM-dependent methyltransferase TRMB-type domain profile.
21 237 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type
21 237 PANTHER PTHR23417 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GT61
AlphaFold — — full sequence — Viewing
ColabFold KP13_02743
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
14 0.757

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 11.26 0.603
2 3.31 0.117
3 2.97 0.097
4 1.98 0.042
5 1.11 0.008