Protein profile

KP13_02197

Single-stranded-DNA-specific exonuclease recJ

Genome: KpKP13

Gene: AHE42766.1 recJ Structure source: AlphaFold + ColabFold UniProt A0A0H3GY37
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Amino acids 577
Annotations 4
Features 19
PDB binders 0
Druggability 0.067

Overview

Basic information about this protein and its source genome.

Accession
KP13_02197
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE42766.1 recJ
Status
annotated
Amino acids
577
Structure source
AlphaFold + ColabFold
GO
GO:0008409 Catalysis of the hydrolysis of ester linkages within nucleic acids by removing nucleotide residues from the 5' end. GO:0003676 Binding to a nucleic acid. GO:0006310 Any process in which a new genotype is formed by reassortment of genes resulting in gene combinations different from those that were present in the parents. In eukaryotes genetic recombination can occur by chromosome assortment, intrachromosomal recombination, or nonreciprocal interchromosomal recombination. Interchromosomal recombination occurs by crossing over. In bacteria it may occur by genetic transformation, conjugation, transduction, or F-duction. GO:0006281 The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
93.15

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.067
Structure A0A0H3GY37
Pocket Pocket 5
P2Rank 0.886
Structure A0A0H3GY37
Pocket Pocket 1
ColabFold model
FPocket 0.504 · Pocket 4
P2Rank 0.715 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 155 / 4744 genomes with a hit
Normalized 0.033

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0008409 Catalysis of the hydrolysis of ester linkages within nucleic acids by removing nucleotide residues from the 5' end.
  • GO:0003676 Binding to a nucleic acid.
  • GO:0006310 Any process in which a new genotype is formed by reassortment of genes resulting in gene combinations different from those that were present in the parents. In eukaryotes genetic recombination can occur by chromosome assortment, intrachromosomal recombination, or nonreciprocal interchromosomal recombination. Interchromosomal recombination occurs by crossing over. In bacteria it may occur by genetic transformation, conjugation, transduction, or F-duction.
  • GO:0006281 The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
24 573 NCBIfam TIGR00644 single-stranded-DNA-specific exonuclease RecJ
24 573 InterPro IPR004610 Bacterial RecJ exonuclease
50 326 FunFam G3DSA:3.90.1640.30:FF:000001 Single-stranded-DNA-specific exonuclease RecJ
327 456 FunFam G3DSA:3.10.310.30:FF:000001 Single-stranded-DNA-specific exonuclease recJ
469 572 Pfam PF17768 RecJ OB domain
469 572 InterPro IPR041122 RecJ, OB domain
207 577 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
188 206 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
315 335 Coils Coil Coil
322 452 Pfam PF02272 DHHA1 domain
322 452 InterPro IPR003156 DHHA1 domain
327 456 Gene3D G3DSA:3.10.310.30 -
73 233 Pfam PF01368 DHH family
73 233 InterPro IPR001667 DDH domain
50 326 Gene3D G3DSA:3.90.1640.30 -
48 465 SUPERFAMILY SSF64182 DHH phosphoesterases
48 465 InterPro IPR038763 DHH phosphoesterase superfamily
1 187 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 576 PANTHER PTHR30255 SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GY37
AlphaFold — — full sequence — Viewing
ColabFold KP13_02197
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 11.55 0.615
2 5.21 0.242
3 4.14 0.168
4 2.52 0.071