Protein profile

KP13_02213

Arsenic efflux pump protein

Genome: KpKP13

Gene: AHE42782.1 arsB Structure source: AlphaFold + ColabFold UniProt A0A0H3GSV2
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Amino acids 430
Annotations 7
Features 58
PDB binders 0
Druggability 0.896

Overview

Basic information about this protein and its source genome.

Accession
KP13_02213
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE42782.1 arsB
Status
annotated
Amino acids
430
Structure source
AlphaFold + ColabFold
GO
GO:0015105 Enables the transfer of arsenite from one side of a membrane to the other. GO:0015700 The directed movement of arsenite into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0042960 Enables the transfer of antimonite from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters. GO:0008490 Enables the transfer of arsenite from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
93.62

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.896
Structure A0A0H3GSV2
Pocket Pocket 8
P2Rank 0.428
Structure A0A0H3GSV2
Pocket Pocket 1
ColabFold model
FPocket 0.595 · Pocket 4
P2Rank 0.39 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 179 / 4744 genomes with a hit
Normalized 0.038

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0015105 Enables the transfer of arsenite from one side of a membrane to the other.
  • GO:0015700 The directed movement of arsenite into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0042960 Enables the transfer of antimonite from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters.
  • GO:0008490 Enables the transfer of arsenite from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters.
  • GO:0046685 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an arsenic stimulus from compounds containing arsenic, including arsenates, arsenites, and arsenides.

Sequence Features

Domain/signature hits from InterPro and related databases.

58 records
Show feature table
Start End DB Term Name
138 160 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
114 131 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
141 151 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 35 Phobius SIGNAL_PEPTIDE Signal peptide region
3 423 Pfam PF02040 Arsenical pump membrane protein
3 423 InterPro IPR000802 Arsenical pump membrane protein, ArsB
101 125 PRINTS PR00758 Arsenical pump membrane protein signature
101 125 InterPro IPR000802 Arsenical pump membrane protein, ArsB
144 168 PRINTS PR00758 Arsenical pump membrane protein signature
144 168 InterPro IPR000802 Arsenical pump membrane protein, ArsB
177 200 PRINTS PR00758 Arsenical pump membrane protein signature
177 200 InterPro IPR000802 Arsenical pump membrane protein, ArsB
364 389 PRINTS PR00758 Arsenical pump membrane protein signature
364 389 InterPro IPR000802 Arsenical pump membrane protein, ArsB
397 421 PRINTS PR00758 Arsenical pump membrane protein signature
397 421 InterPro IPR000802 Arsenical pump membrane protein, ArsB
278 302 PRINTS PR00758 Arsenical pump membrane protein signature
278 302 InterPro IPR000802 Arsenical pump membrane protein, ArsB
217 242 PRINTS PR00758 Arsenical pump membrane protein signature
217 242 InterPro IPR000802 Arsenical pump membrane protein, ArsB
323 345 PRINTS PR00758 Arsenical pump membrane protein signature
323 345 InterPro IPR000802 Arsenical pump membrane protein, ArsB
173 177 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
316 334 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
3 412 CDD cd01118 ArsB_permease
3 412 InterPro IPR000802 Arsenical pump membrane protein, ArsB
264 274 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
91 110 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
229 422 PANTHER PTHR43302 TRANSPORTER ARSB-RELATED
1 1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
3 426 NCBIfam TIGR00935 arsenite/antimonite efflux transporter
3 426 InterPro IPR000802 Arsenical pump membrane protein, ArsB
175 197 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
275 296 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
335 400 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
116 140 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
111 115 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
36 50 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
71 90 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
51 70 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
241 245 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
91 110 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
277 296 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
246 263 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
18 35 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
152 172 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
401 423 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
316 335 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
199 221 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
228 262 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
2 17 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
424 430 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
178 198 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
406 428 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
297 315 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
48 70 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
222 240 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GSV2
AlphaFold — — full sequence — Viewing
ColabFold KP13_02213
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
8 0.896
2 0.782
15 0.55
14 0.285

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 5.79 0.282
2 5.75 0.28
3 5.42 0.257
4 3.6 0.134
5 1.61 0.026