Protein profile

KP13_02343

Membrane-bound lytic murein transglycosylase A

Genome: KpKP13

Gene: mltA AHE42913.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GUL3
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Amino acids 365
Annotations 8
Features 28
PDB binders 0
Druggability 0.618

Overview

Basic information about this protein and its source genome.

Accession
KP13_02343
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
mltA AHE42913.1
Status
annotated
Amino acids
365
Structure source
AlphaFold + ColabFold
EC
4.2.2.n1
GO
GO:0004553 Catalysis of the hydrolysis of any O-glycosyl bond. GO:0009254 The continual breakdown and regeneration of peptidoglycan required to maintain the bacterial cell wall. Peptidoglycans consist of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides. GO:0019867 The external membrane of Gram-negative bacteria or certain organelles such as mitochondria and chloroplasts; freely permeable to most ions and metabolites. GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures. GO:0008933 Catalysis of the cleavage of a peptidoglycan chain into a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end. Includes endolytic transglycosylase activity that fragments the glycan chain internally and exolytic transgylcosylase activity that cleaves a terminal disaccharide from the end of the glycan strand. GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
94.247
DEG E-value
0.0
Localization
OuterMembrane
ColabFold pLDDT
92.17

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.618
Structure A0A0H3GUL3
Pocket Pocket 1
P2Rank 0.409
Structure A0A0H3GUL3
Pocket Pocket 1
ColabFold model
FPocket 0.18 · Pocket 21
P2Rank 0.565 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 121 / 4744 genomes with a hit
Normalized 0.026

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0004553 Catalysis of the hydrolysis of any O-glycosyl bond.
  • GO:0009254 The continual breakdown and regeneration of peptidoglycan required to maintain the bacterial cell wall. Peptidoglycans consist of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.
  • GO:0019867 The external membrane of Gram-negative bacteria or certain organelles such as mitochondria and chloroplasts; freely permeable to most ions and metabolites.
  • GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures.
  • GO:0008933 Catalysis of the cleavage of a peptidoglycan chain into a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end. Includes endolytic transglycosylase activity that fragments the glycan chain internally and exolytic transgylcosylase activity that cleaves a terminal disaccharide from the end of the glycan strand.
  • GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.
  • GO:0009253 The chemical reactions and pathways resulting in the breakdown of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.

Sequence Features

Domain/signature hits from InterPro and related databases.

28 records
Show feature table
Start End DB Term Name
261 353 CDD cd22785 DPBB_MltA-like
125 262 Gene3D G3DSA:2.40.240.50 -
1 21 ProSiteProfiles PS51257 Prokaryotic membrane lipoprotein lipid attachment site profile.
1 362 PANTHER PTHR30124 MEMBRANE-BOUND LYTIC MUREIN TRANSGLYCOSYLASE A
1 362 InterPro IPR026044 Membrane-bound lytic murein transglycosylase A
25 353 SUPERFAMILY SSF50685 Barwin-like endoglucanases
25 353 InterPro IPR036908 RlpA-like domain superfamily
1 23 SignalP_EUK SignalP-noTM SignalP-noTM
277 354 Pfam PF06725 3D domain
277 354 InterPro IPR010611 3D domain
24 365 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 23 Phobius SIGNAL_PEPTIDE Signal peptide region
1 8 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
125 262 FunFam G3DSA:2.40.240.50:FF:000001 Membrane-bound lytic murein transglycosylase A
43 354 Gene3D G3DSA:2.40.40.10 -
43 354 InterPro IPR036908 RlpA-like domain superfamily
20 23 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
9 19 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
125 257 SMART SM00925 MltA_2
125 257 InterPro IPR005300 Lytic transglycosylase MltA, domain B
126 259 CDD cd14472 mltA_B_like
85 257 Pfam PF03562 MltA specific insert domain
85 257 InterPro IPR005300 Lytic transglycosylase MltA, domain B
1 22 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
1 147 PIRSF PIRSF019422 MltA
1 147 InterPro IPR026044 Membrane-bound lytic murein transglycosylase A
145 365 PIRSF PIRSF019422 MltA
145 365 InterPro IPR026044 Membrane-bound lytic murein transglycosylase A

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GUL3
AlphaFold — — full sequence — Viewing
ColabFold KP13_02343
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.618

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 5.09 0.233
2 4.75 0.209