Protein profile

KP13_02587

putative High-affinity nickel transport protein

Genome: KpKP13

Gene: AHE43048.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GWN2
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Amino acids 337
Annotations 4
Features 31
PDB binders 0
Druggability 0.766

Overview

Basic information about this protein and its source genome.

Accession
KP13_02587
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE43048.1
Status
annotated
Amino acids
337
Structure source
AlphaFold + ColabFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0035444 The directed movement of nickel (Ni) cations across a membrane by means of some agent such as a transporter or pore. GO:0015099 Enables the transfer of nickel (Ni) cations from one side of a membrane to the other. GO:0012505 A collection of membranous structures involved in transport within the cell. The main components of the endomembrane system are endoplasmic reticulum, Golgi bodies, vesicles, cell membrane and nuclear envelope. Members of the endomembrane system pass materials through each other or though the use of vesicles.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
43.607
DEG E-value
1.43e-78
Localization
CytoplasmicMembrane
ColabFold pLDDT
81.8

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.766
Structure A0A0H3GWN2
Pocket Pocket 5
P2Rank 0.876
Structure A0A0H3GWN2
Pocket Pocket 1
ColabFold model
FPocket 0.807 · Pocket 9
P2Rank 0.905 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 67 / 4744 genomes with a hit
Normalized 0.014

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0035444 The directed movement of nickel (Ni) cations across a membrane by means of some agent such as a transporter or pore.
  • GO:0015099 Enables the transfer of nickel (Ni) cations from one side of a membrane to the other.
  • GO:0012505 A collection of membranous structures involved in transport within the cell. The main components of the endomembrane system are endoplasmic reticulum, Golgi bodies, vesicles, cell membrane and nuclear envelope. Members of the endomembrane system pass materials through each other or though the use of vesicles.

Sequence Features

Domain/signature hits from InterPro and related databases.

31 records
Show feature table
Start End DB Term Name
77 99 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
41 325 Pfam PF03824 High-affinity nickel-transport protein
41 325 InterPro IPR011541 Nickel/cobalt transporter, high-affinity
258 285 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 32 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
229 251 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
77 100 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
186 210 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
146 185 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
101 119 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
39 56 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
119 141 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
216 238 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 34 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
328 337 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
263 285 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
45 320 NCBIfam TIGR00802 HoxN/HupN/NixA family nickel/cobalt transporter
45 320 InterPro IPR004688 Transition metal uptake transporter nickel/cobalt
305 327 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
38 56 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
33 37 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
8 330 PANTHER PTHR31611 HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1
8 330 InterPro IPR004688 Transition metal uptake transporter nickel/cobalt
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
120 145 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
305 327 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
57 76 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
211 215 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
286 304 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
187 209 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
239 257 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GWN2
AlphaFold — — full sequence — Viewing
ColabFold KP13_02587
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
5 0.766

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 16.17 0.772
2 12.87 0.666
3 3.66 0.138
4 1.91 0.039
5 1.52 0.022