Protein target profile

KP13_02458

putative fimbrial biogenesis outer membrane usher protein

Genome: KpKP13 Gene: AHE43106.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A6A8ECN8
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Length 845
Pocket druggability 0.918
Functional annotation 0 EC 5 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Strong predicted binding pocket FPocket selected-pocket score 0.92. This is a computational pocket signal, not binding validation. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 1 of 4744 screened genomes (0.0%) have a significant similarity hit. This target also has a predicted binding pocket (FPocket 0.92) — check the ligand evidence before deprioritizing on off-target risk alone. Mixed conservation Core/accessory calls are not fully concordant. Weak second pocket-predictor signal P2Rank predicted binding-site probability 0.24.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
0.0% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Localization

Localization
OuterMembrane

Structure confidence

ColabFold pLDDT
86.09 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket 0.918
Structure A0A6A8ECN8
Pocket Pocket 7
P2Rank 0.24
Structure A0A6A8ECN8
Pocket Pocket 1
ColabFold model
FPocket 0.132 · Pocket 7
P2Rank 0.338 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 1 / 4744 genomes with a hit
Prevalence 0.0%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A6A8ECN8

Sequence

Primary amino-acid sequence viewer.

MDDSKLRYFASAWLISITLPADSAERYNAQFVNGIDPLAFNQFVASDGDVMPGTYDVNIYINDLLVDSRPVRFSEDSAHGGLAPCLSAAEYIRYGVKIDDDHQPCFALSQTIRQAEQQLDIANHRLIIHIPQQYIEHYPRDYVSPMRFDEGINAAFVNYSYSTDANNGDGGSHQYQYLSLNSGINIASWRLRNNAYWNKFSGQADKWQSIASWAETNIIPWRSRLVVGQTSTDNSVFDSVQFRGVQLGTDVEMRPSSQTGFAPVIRGVANSNARVEVRQNNYLIYSENVPAGPFELNDISAVNRSGDFYVTVIEADGSQTTFTVAYTTLPQLVRAGQWNYQLSAGKYHDGADGYAPALMQSSLSYGLNNTFTLYGGALAAENYRAGAFGVGSNLGEIGALSADYTLAGTTLANGQRKQGGSVRFLYAKSFLSSKTDFQIAGYRYSTAGYYSLSDAVNERRRWHNGLYENDYWPSDEDESWQASAPQHYYTSWFYNKKHRFDISARQTLGKNSAFFLNFSQQNYWNSSGSDISLQAGFNSTIHNVNYGLYYQNTRSHFTHDDNSITLRVSIPFTLQEDRRINTAFTLAHSKSSGTSGQAGVNGTLLDDGRLSWAVTSAYDDTSHSTNSASLGYLGQYGNLYTGYAYSKSHRQASLNLSGGVVAHRGGVTLSQPLGSTFALVEAKDAQGVGIENQTGVRIDPFGYAVVPQSVPYRVNSVALNPQDFDAFLDVPNAVADTVPTRGAITRVRFDTFRGYSVLIHTTLADGSYPPLGAQLYRASGISNGLVGPGGEVYVSGVDSGEKLQIKWGETHQQSCEITLPELRQEPQQATAWRELSLICTVTPSR

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0005515 Binding to a protein.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0015473 A porin that acts in the assembly of fimbria together with fimbrial chaperone.
  • GO:0009297 The assembly from its constituent parts of a pilus, a short filamentous structure of bacterial cell, flagella-like in structure and generally present in many copies. Pili are variously involved in transfer of nucleic acids, adherence to surfaces, and formation of pellicles. Is required for bacterial conjugation, or can play a role in adherence to surfaces (when it is called a fimbrium), and in the formation of pellicles.
  • GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

25 records
Show feature table
Start End DB Term Name
40 151 Gene3D G3DSA:3.10.20.410 -
40 151 InterPro IPR037224 PapC, N-terminal domain superfamily
248 336 Gene3D G3DSA:2.60.40.3110 -
6 839 PANTHER PTHR30451 OUTER MEMBRANE USHER PROTEIN
6 839 InterPro IPR000015 Outer membrane usher protein
24 845 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
247 336 FunFam G3DSA:2.60.40.3110:FF:000001 Putative fimbrial outer membrane usher
1 23 Phobius SIGNAL_PEPTIDE Signal peptide region
1 8 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
755 842 Gene3D G3DSA:2.60.40.2070 -
755 842 InterPro IPR043142 PapC-like, C-terminal domain superfamily
20 23 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
9 19 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
664 754 Gene3D G3DSA:2.60.40.2610 Outer membrane usher protein FimD, plug domain
664 754 InterPro IPR042186 Outer membrane usher protein FimD, plug domain
758 818 Pfam PF13953 PapC C-terminal domain
758 818 InterPro IPR025949 PapC-like, C-terminal domain
48 154 SUPERFAMILY SSF141729 FimD N-terminal domain-like
48 154 InterPro IPR037224 PapC, N-terminal domain superfamily
177 749 Pfam PF00577 Outer membrane usher protein
177 749 InterPro IPR000015 Outer membrane usher protein
39 162 Pfam PF13954 PapC N-terminal domain
39 162 InterPro IPR025885 PapC, N-terminal domain
289 299 ProSitePatterns PS01151 Fimbrial biogenesis outer membrane usher protein signature.
289 299 InterPro IPR018030 Fimbrial membrane usher, conserved site

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Site 1 FPocket #7
0.918
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Surrounding area
Site 2 FPocket #41
0.422
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Surrounding area
Site 3 FPocket #9
0.291
Unusual size
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Surrounding area

Binding pockets · P2Rank

Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Site 1 P2Rank #1
0.24
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Surrounding area
Site 2 P2Rank #2
0.219
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Surrounding area
Site 3 P2Rank #3
0.2
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Surrounding area
Site 4 P2Rank #4
0.119
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Surrounding area
Site 5 P2Rank #5
0.118
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A6A8ECN8
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_02458
ColabFold — — full sequence — Loaded