Protein profile

KP13_00802

Autonomous glycyl radical cofactor

Genome: KpKP13

Gene: grcA AHE43167.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GW99

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Amino acids 127

Annotations 3

Features 15

PDB binders 0

Druggability 0.079

Overview

Basic information about this protein and its source genome.

Accession: KP13_00802
Assembly: Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene: grcA AHE43167.1
Status: annotated
Amino acids: 127
Structure source: AlphaFold + ColabFold

GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0008861 Catalysis of the reaction: acetyl-CoA + formate = CoA + pyruvate.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Human identity (%): 0.0
Gut microbiome off-target: hit
Essential (DEG): Y
DEG identity (%): 92.126
DEG E-value: 5.76e-86
Localization: Cytoplasmic
ColabFold pLDDT: 89.35

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.079

Structure A0A0H3GW99

Pocket Pocket 2

P2Rank

Structure A0A0H3GW99

Pocket No pockets

ColabFold model

FPocket 0.129 · Pocket 4

Core conservation Conserved core gene

Roary core

CoreCruncher core

Gut microbiome 216 / 4744 genomes with a hit

Normalized 0.046

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO:0008861 Catalysis of the reaction: acetyl-CoA + formate = CoA + pyruvate.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records

Show feature table

Start	End	DB	Term	Name
67	126	PANTHER	PTHR30191	FORMATE ACETYLTRANSFERASE
64	126	FunFam	G3DSA:3.20.70.20:FF:000002	Autonomous glycyl radical cofactor
5	127	ProSiteProfiles	PS51149	Glycine radical domain profile.
5	127	InterPro	IPR001150	Glycine radical domain
1	127	Hamap	MF_00806	Autonomous glycyl radical cofactor [grcA].
1	127	InterPro	IPR011140	Autonomous glycyl radical cofactor GrcA
97	105	ProSitePatterns	PS00850	Glycine radical domain signature.
97	105	InterPro	IPR019777	Formate C-acetyltransferase glycine radical, conserved site
64	126	SUPERFAMILY	SSF51998	PFL-like glycyl radical enzymes
2	127	NCBIfam	TIGR04365	autonomous glycyl radical cofactor GrcA
2	127	InterPro	IPR011140	Autonomous glycyl radical cofactor GrcA
2	127	PIRSF	PIRSF000378	Gly_radicl_YfiD
64	126	Gene3D	G3DSA:3.20.70.20	-
16	108	Pfam	PF01228	Glycine radical
16	108	InterPro	IPR001150	Glycine radical domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

No pockets are loaded yet for the displayed AlphaFold model AF_A0A0H3GW99 structure. Run experimental pocket backfill to show FPocket/P2Rank overlays on this structure.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold AF_A0A0H3GW99	AlphaFold	—	—	full sequence	—	Viewing
ColabFold KP13_00802	ColabFold	—	—	full sequence	—	Loaded