Protein profile

KP13_00825

Putative sensor-like histidine kinase

Genome: KpKP13

Gene: AHE43189.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GRS8
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Amino acids 479
Annotations 4
Features 38
PDB binders 4
Druggability 0.706

Overview

Basic information about this protein and its source genome.

Accession
KP13_00825
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE43189.1
Status
annotated
Amino acids
479
Structure source
AlphaFold + ColabFold
GO
GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
86.55

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.706
Structure A0A0H3GRS8
Pocket Pocket 3
P2Rank 0.933
Structure A0A0H3GRS8
Pocket Pocket 1
ColabFold model
FPocket 0.785 · Pocket 2
P2Rank 0.935 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 110 / 4744 genomes with a hit
Normalized 0.023

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Sequence Features

Domain/signature hits from InterPro and related databases.

38 records
Show feature table
Start End DB Term Name
262 478 ProSiteProfiles PS50109 Histidine kinase domain profile.
262 478 InterPro IPR005467 Histidine kinase domain
366 478 SMART SM00387 HKATPase_4
366 478 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
1 19 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
199 315 Gene3D G3DSA:1.10.287.130 -
255 323 SMART SM00388 HisKA_10
255 323 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
181 203 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
205 479 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
20 40 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
253 319 CDD cd00082 HisKA
253 319 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
371 476 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
371 476 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
41 180 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
247 478 PANTHER PTHR43711 TWO-COMPONENT HISTIDINE KINASE
256 322 Pfam PF00512 His Kinase A (phospho-acceptor) domain
256 322 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
300 320 Coils Coil Coil
181 204 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
234 321 FunFam G3DSA:1.10.287.130:FF:000039 Sensor-like histidine kinase YfhK
403 417 PRINTS PR00344 Bacterial sensor protein C-terminal signature
403 417 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
438 456 PRINTS PR00344 Bacterial sensor protein C-terminal signature
438 456 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
462 475 PRINTS PR00344 Bacterial sensor protein C-terminal signature
462 475 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
421 431 PRINTS PR00344 Bacterial sensor protein C-terminal signature
421 431 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
328 476 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
328 476 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
20 37 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
316 476 Gene3D G3DSA:3.30.565.10 -
316 476 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
242 323 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
242 323 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
371 474 CDD cd00075 HATPase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GRS8
AlphaFold — — full sequence — Viewing
ColabFold KP13_00825
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.706
1 0.65
13 0.445

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 20.87 0.863
2 1.58 0.024

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

54 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
EMC
3A0W
Q9X180 229.7 Da LogP 0.97 TPSA 0.0 ✓ Ro5 ✓ Clean CC[Hg+]
EMT
3A0W
Q9X180 382.8 Da LogP 2.91 TPSA 37.3 ✓ Ro5 ✓ Clean CC[Hg]Sc1ccccc1C(=O)O
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.