KpKP13 Protein target profile

putative 3-phenylpropionic acid transporter

Accession: KP13_00837

Gene: hcaT AHE43201.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A6TCF9
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Length 378
Pocket druggability (P2Rank · AlphaFold DB model) 0.945
Functional annotation 0 EC 0 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.94, FPocket 0.87). Use the 3D pocket comparison to verify whether they identify the same site. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.87. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 108 of 4744 screened genomes (2.3%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.94) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.3% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
92.57 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.945
Structure A6TCF9
Pocket Pocket 1
Druggability (FPocket) 0.87
Structure A6TCF9
Pocket Pocket 1
ColabFold model
P2Rank 0.948 · Pocket 1
FPocket 0.403 · Pocket 16
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 108 / 4744 genomes with a hit
Prevalence 2.3%

Sequence

Primary amino-acid sequence viewer.

MVLHSTRWLALSYFTYFFSYGIFLPFWSVWLAGNGLTPETIGILLGAGLVARFLGSLLIAPRVSDPSRLIAALRVLALLTLLFALAFWAGSHVAWLLAIIIGFNLFFSPLVPLTDALANTWQKQITMDYGRVRLWGSIAFVIGSALTGKLVSLFDYRAILLMLSLGVASMLLGMLLKPSVMPQGESRQQQGAGMAAWLTLVRQSWRFLACVCLLQGAHAAYYGFSAIYWQQAGYSASAVGYLWSLGVVAEVVIFALSKKVFRRFSARDLLLLSAVCGLIRWGLMGWTTALPGLILAQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVAMGGSIAIMTVFAGFLYQHLHQGVFWVMALLTLPAMAIRPKAVAA

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

Subcellular localization

Localization
CytoplasmicMembrane

No GO or EC annotations are currently loaded for this protein.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

50 records
Show feature table
Start End DB Term Name
194 372 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
194 372 InterPro IPR036259 MFS transporter superfamily
234 256 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
70 89 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
293 315 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
132 154 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
241 257 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
351 355 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
114 133 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
90 94 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
8 356 Pfam PF12832 MFS_1 like family
8 356 InterPro IPR024989 Major facilitator superfamily associated domain
95 113 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
317 327 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
328 350 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
207 229 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
71 89 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
373 378 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
258 268 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
4 188 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
4 188 InterPro IPR036259 MFS transporter superfamily
9 369 NCBIfam NF037955 MFS transporter
134 152 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
158 176 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
158 180 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
9 31 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
355 372 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
41 63 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
60 70 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
289 293 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
7 28 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
153 157 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
328 350 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
269 288 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
269 288 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
189 373 FunFam G3DSA:1.20.1250.20:FF:000046 3-phenylpropionic acid MFS transporter
230 240 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
356 372 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
294 316 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
207 229 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
93 111 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
4 375 PANTHER PTHR23522 BLL5896 PROTEIN
40 59 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
7 370 SUPERFAMILY SSF103473 MFS general substrate transporter
7 370 InterPro IPR036259 MFS transporter superfamily
1 378 PIRSF PIRSF004925 HcaT
1 378 InterPro IPR026032 Probable 3-phenylpropionic acid transporter-like
29 39 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
177 206 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.945
Likely same site as FPocket 1 1.1 Å 27 shared residues 84% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.078
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Surrounding area
Pocket 3 P2Rank #3
0.045
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Surrounding area
Pocket 4 P2Rank #4
0.032
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Surrounding area
Pocket 5 P2Rank #5
0.019
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #1
0.87 Unusual size
Likely same site as P2Rank 1 1.1 Å 27 shared residues 84% of smaller site
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Surrounding area
Pocket 2 FPocket #14
0.42
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A6TCF9
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_00837
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A6TCF9