KpKP13 Protein target profile

putative lipoprotein

Accession: KP13_00856

Gene: AHE43219.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GRN4
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Length 1649
Pocket druggability (P2Rank · AlphaFold DB model) 0.759
Functional annotation 0 EC 2 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Second pocket predictor agrees P2Rank predicted binding-site probability 0.76. Structure available AlphaFold DB model + ColabFold model

Risks to review

Low human similarity A human hit was flagged, but identity is only 26.119% - a weak match, lower risk than a close homolog. This target also has a predicted binding pocket (P2Rank 0.76) — check the ligand evidence before deprioritizing on off-target risk alone. Microbiome similarity 105 of 4744 screened genomes (2.2%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.76) — check the ligand evidence before deprioritizing on off-target risk alone. Weak predicted binding pocket FPocket selected-pocket score 0.31.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
Hit
Human identity (%)
26.119 Lower values reduce human off-target concern.
Human E-value
3.54e-06
Gut microbiome similarity
2.2% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
83.91 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.759
Structure A0A0H3GRN4
Pocket Pocket 1
Druggability (FPocket) 0.307
Structure A0A0H3GRN4
Pocket Pocket 1
ColabFold model
P2Rank 0.85 · Pocket 1
FPocket 0.53 · Pocket 142
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 105 / 4744 genomes with a hit
Prevalence 2.2%

Sequence

Primary amino-acid sequence viewer.

MKPFRLAALSLALLTAFSLTGCDDSGTPQASAPAPAADSNPGATAKPDRAQLAALAEKSQGKALTLLDASEVQLDGAATLVLTFSVPLQPDQDFSRSVHLVDKKSGKVDGAWELAPNLKELRLRHLEPKRELIVSVDPTLTALNKATLDKPFEKTLTTRDIAPSVGFASRGSLLPGNVVAGLPVMALNVDNVDVNFFRIKPESLSAFVSQWEYRNSLSNWESDELLKMADLVYTGRFDLNPARNTREKLLLPLSDIKPLQQPGVYVAVMNPAGRYSYSNAATLFTLSDIGVSAHRYHNRLDVFTQSLENGAAQSGIEVQLLNAKGQTLAEAKSDSQGHVTLQTDKEAALLLARKEGQTTLLDLKLPALDLAEFSIAGAPGFSKQFFMFGPRDLYRPGETVILNALLRDSDGKPLAEQPVKLEVVQPDGQVIRSVMSKPVNGLYQFTYPLDSGAATGMWHIRASAGDNQPREWDFHVEDFMPERMALNLTPQAAPVAPDADVTFGVSGAYLYGAPASGNQLQGKLFLRPLRDAVAALPGFQFGDIAEENLSRSLDEVQLTLDEKGHGEVTTSSQWQDSHSPLQVVLQASLLESGGRPVTRTVKQPIWPAEALPGIRPQFTLKDVYDYRTDTTVKQPVVDENSNAAFDIVYADVKGEKKAISGLQVRLIRERRDYYWNWSDSEGWQSQFDQKDLQEGEESLDLQAGQIGKVSFPVEWGSYRLEVKGSDDVVSSVRFWAGYSWQDNSEGAGAARPDRVTMKLDKPSYKPGDTIRLHIAAPAAGKGYAMVESSEGPLWWQEIDVPAEGMDLSIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGLLHLPLGDENRRLTLALEAPDKMRPNQPLTVKVKASVKEGEAPKQVNVLLSAVDSGVLNITDYATPDPWNAFFGQKRYGADIYDIYGQVIEGQGRVASLRFGGDGDELKRGGKPPVNHVTIVAQQAQPVVLNDQGEGTVTLPIGDFNGELRVMAQAWTADDFGSSEDKVVVAAPVIAELNTPRFLASGDTTRLALDLSNLTDKPQTLQVHLTASGLVTLTEGQLPPVQLAPGARSTLFIPVSALAGFGDGQVNATISGLSLPGETFAPLQKQWKIGVRPAYPAQTVNSGAVLQPGESWQPPAAQSQGFAPQTLQGQLLLSGKPPLNLARYIRELKAYPYGCLEQTASGLFPSLYTSAAQLKALGISGDSDEKRRAAIDVGISRLLQMQLENGGFALWDREGPEEYWLTAYAMDFLVRASEQGYSVPVNAINKGNERLLRYLQEPGLMTVRYSDDAQASRFAAQAYAALVLARQQKAPLGALREIWSRHDQARSGLPLLQLGIALKTMGDAPRGDAALKLAVATPRQDENRWLGDYGSPLRDNALKLALLEENKLLPEVQNQLLSTLSEEAYGQRWLSTQETNALFLAGRTLADLPGSWQAKTSLQAEPLAGDKAQTRNLDGDRLAALQVSNTGSQPLWLRLDSSGYPQSAPQPGGNVLGIERTIFDTQGQQKSLSSLRSGELVLVKLEVTAKRNVPDALVVDLLPAGLELENQNLANSSASLQENGDAVQNLLNQMQQADIQHIEFRDDRFVAAVAVNEGQPVTLVYLARAVTPGTYQVPQPQVESMYAPQWRATGAASGSLTVTP

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 GO

Subcellular localization

Localization
Unknown

Gene Ontology (GO)

2
  • GO:0004866 Binds to and stops, prevents or reduces the activity of an endopeptidase.
  • GO:0005615 OBSOLETE. That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

41 records
Show feature table
Start End DB Term Name
1170 1434 CDD cd02891 A2M_like
1 30 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
482 607 Pfam PF17972 Bacterial Alpha-2-macroglobulin MG5 domain
482 607 InterPro IPR041203 Bacterial Alpha-2-macroglobulin, MG5 domain
1 1649 PIRSF PIRSF038980 A2M_bac
1 1649 InterPro IPR026284 Alpha-2-macroglobulin, bacteria
965 1054 SMART SM01360 A2M_2
965 1054 InterPro IPR001599 Alpha-2-macroglobulin
385 476 Pfam PF01835 MG2 domain
385 476 InterPro IPR002890 Macroglobulin domain
286 378 Pfam PF11974 Bacterial alpha-2-macroglobulin MG3 domain
286 378 InterPro IPR021868 Alpha-2-macroglobulin MG3 domain
756 902 Pfam PF07703 Alpha-2-macroglobulin bait region domain
756 902 InterPro IPR011625 Alpha-2-macroglobulin, bait region domain
1174 1430 SUPERFAMILY SSF48239 Terpenoid cyclases/Protein prenyltransferases
1174 1430 InterPro IPR008930 Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid
1173 1202 SMART SM01419 Thiol_ester_cl_2
1173 1202 InterPro IPR047565 Alpha-macroglobulin-like, thiol-ester bond-forming region
384 480 Gene3D G3DSA:2.60.40.1930 -
56 160 Pfam PF17970 Bacterial Alpha-2-macroglobulin MG1 domain
56 160 InterPro IPR040639 Alpha-2-macroglobulin, MG1 domain
1501 1634 Pfam PF17973 Bacterial Alpha-2-macroglobulin MG10 domain
1501 1634 InterPro IPR041246 Bacterial alpha-2-macroglobulin MG10 domain
1 5 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
1174 1283 Pfam PF07678 A-macroglobulin TED domain
1174 1283 InterPro IPR011626 Alpha-macroglobulin-like, TED domain
6 17 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
56 1641 PANTHER PTHR40094 ALPHA-2-MACROGLOBULIN HOMOLOG
613 738 Pfam PF17962 Bacterial macroglobulin domain 6
613 738 InterPro IPR041462 Bacterial Alpha-2-macroglobulin, MG6 domain
18 22 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 22 SignalP_EUK SignalP-noTM SignalP-noTM
1169 1436 Gene3D G3DSA:1.50.10.20 -
755 903 SMART SM01359 A2M_N_2_2
755 903 InterPro IPR011625 Alpha-2-macroglobulin, bait region domain
973 1054 Pfam PF00207 Alpha-2-macroglobulin family
973 1054 InterPro IPR001599 Alpha-2-macroglobulin
1 22 Phobius SIGNAL_PEPTIDE Signal peptide region
1 21 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
23 1649 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 22 ProSiteProfiles PS51257 Prokaryotic membrane lipoprotein lipid attachment site profile.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.759
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Surrounding area
Pocket 2 P2Rank #2
0.558
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Surrounding area
Pocket 3 P2Rank #3
0.501
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Surrounding area
Pocket 4 P2Rank #4
0.4
Likely same site as FPocket 1 1.2 Å 15 shared residues 94% of smaller site
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Surrounding area
Pocket 5 P2Rank #5
0.28
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #1
0.307
Likely same site as P2Rank 4 1.2 Å 15 shared residues 94% of smaller site
Show in viewer
Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GRN4
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_00856
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GRN4