Protein profile

KP13_00934

Polyphosphate kinase

Genome: KpKP13

Gene: AHE43295.1 ppk Structure source: AlphaFold + ColabFold UniProt A0A0H3GW20
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Amino acids 686
Annotations 7
Features 34
PDB binders 0
Druggability 0.509

Overview

Basic information about this protein and its source genome.

Accession
KP13_00934
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE43295.1 ppk
Status
annotated
Amino acids
686
Structure source
AlphaFold + ColabFold
EC
2.7.4.1
GO
GO:0009358 A protein complex that possesses polyphosphate kinase activity. GO:0008976 Catalysis of the reaction: ATP + phosphate(n) = ADP + phosphate(n+1). GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. GO:0006799 The chemical reactions and pathways resulting in the formation of a polyphosphate, the anion or salt of polyphosphoric acid. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0046872 Binding to a metal ion.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
79.592
DEG E-value
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
90.93

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.509
Structure A0A0H3GW20
Pocket Pocket 2
P2Rank 0.906
Structure A0A0H3GW20
Pocket Pocket 1
ColabFold model
FPocket 0.343 · Pocket 9
P2Rank 0.908 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 136 / 4744 genomes with a hit
Normalized 0.029

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0009358 A protein complex that possesses polyphosphate kinase activity.
  • GO:0008976 Catalysis of the reaction: ATP + phosphate(n) = ADP + phosphate(n+1).
  • GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
  • GO:0006799 The chemical reactions and pathways resulting in the formation of a polyphosphate, the anion or salt of polyphosphoric acid.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0046872 Binding to a metal ion.

Sequence Features

Domain/signature hits from InterPro and related databases.

34 records
Show feature table
Start End DB Term Name
331 493 Pfam PF17941 Polyphosphate kinase C-terminal domain 1
331 493 InterPro IPR041108 Polyphosphate kinase, C-terminal domain 1
501 672 Pfam PF13090 Polyphosphate kinase C-terminal domain 2
501 672 InterPro IPR025200 Polyphosphate kinase C-terminal domain 2
501 682 SUPERFAMILY SSF56024 Phospholipase D/nuclease
7 109 Pfam PF13089 Polyphosphate kinase N-terminal domain
7 109 InterPro IPR025198 Polyphosphate kinase N-terminal domain
3 105 SUPERFAMILY SSF140356 PPK N-terminal domain-like
3 105 InterPro IPR036832 Polyphosphate kinase N-terminal domain superfamily
1 685 PIRSF PIRSF015589 PPK_bact
1 685 InterPro IPR003414 Polyphosphate kinase
110 307 Gene3D G3DSA:3.30.1840.10 Polyphosphate kinase middle domain
110 307 InterPro IPR036830 Polyphosphate kinase middle domain superfamily
500 686 FunFam G3DSA:3.30.870.10:FF:000007 Polyphosphate kinase
6 681 Hamap MF_00347 Polyphosphate kinase [ppk].
6 681 InterPro IPR003414 Polyphosphate kinase
119 321 Pfam PF02503 Polyphosphate kinase middle domain
119 321 InterPro IPR024953 Polyphosphate kinase middle domain
319 499 Gene3D G3DSA:3.30.870.10 Endonuclease Chain A
3 682 PANTHER PTHR30218 POLYPHOSPHATE KINASE
3 682 InterPro IPR003414 Polyphosphate kinase
428 462 ProSiteProfiles PS50035 Phospholipase D phosphodiesterase active site profile.
428 462 InterPro IPR001736 Phospholipase D/Transphosphatidylase
498 662 CDD cd09167 PLDc_EcPPK1_C2_like
319 499 FunFam G3DSA:3.30.870.10:FF:000001 Polyphosphate kinase
107 313 SUPERFAMILY SSF143724 PHP14-like
9 109 FunFam G3DSA:1.20.58.310:FF:000001 Polyphosphate kinase
329 490 CDD cd09164 PLDc_EcPPK1_C1_like
110 307 FunFam G3DSA:3.30.1840.10:FF:000001 Polyphosphate kinase
7 677 NCBIfam TIGR03705 polyphosphate kinase 1
7 677 InterPro IPR003414 Polyphosphate kinase
9 109 Gene3D G3DSA:1.20.58.310 -
500 686 Gene3D G3DSA:3.30.870.10 Endonuclease Chain A
315 496 SUPERFAMILY SSF56024 Phospholipase D/nuclease

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GW20
AlphaFold — — full sequence — Viewing
ColabFold KP13_00934
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.509
1 0.291
3 0.007
12 0.002

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 30.83 0.906
2 23.59 0.843
3 6.14 0.253
4 5.63 0.225
5 2.37 0.051