Protein profile

KP13_03828

tRNA(Met) cytidine acetyltransferase TmcA

Genome: KpKP13

Gene: AHE43312.1 tmcA Structure source: AlphaFold + ColabFold UniProt A0A486QV08
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Amino acids 667
Annotations 11
Features 25
PDB binders 0
Druggability 0.411

Overview

Basic information about this protein and its source genome.

Accession
KP13_03828
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE43312.1 tmcA
Status
annotated
Amino acids
667
Structure source
AlphaFold + ColabFold
EC
2.3.1.193
GO
GO:0051391 The modification of tRNA structure by addition of an acetyl group to tRNA. An acetyl group is CH3CO-, derived from acetic [ethanoic] acid. GO:0002101 The process in which a cytosine at position 34 of a tRNA is post-transcriptionally modified. The wobble nucleoside of the tRNA sequence (position 34) corresponds to the first position of the anticodon. GO:0000049 Binding to a transfer RNA. GO:0016747 Catalysis of the transfer of an acyl group, other than amino-acyl, from one compound (donor) to another (acceptor). GO:0008080 Catalysis of the transfer of an acetyl group to a nitrogen atom on the acceptor molecule. GO:0051392 Catalysis of the reaction: a cytidine in tRNA + acetyl-CoA + ATP + H2O = ADP + an N4-acetylcytidine in tRNA + CoA + H+ + phosphate.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
23.399
Human E-value
2.83e-17
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
90.87

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.411
Structure A0A486QV08
Pocket Pocket 4
P2Rank 0.798
Structure A0A486QV08
Pocket Pocket 1
ColabFold model
FPocket 0.476 · Pocket 7
P2Rank 0.9 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 87 / 4744 genomes with a hit
Normalized 0.018

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 10 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

10
  • GO:0051391 The modification of tRNA structure by addition of an acetyl group to tRNA. An acetyl group is CH3CO-, derived from acetic [ethanoic] acid.
  • GO:0002101 The process in which a cytosine at position 34 of a tRNA is post-transcriptionally modified. The wobble nucleoside of the tRNA sequence (position 34) corresponds to the first position of the anticodon.
  • GO:0000049 Binding to a transfer RNA.
  • GO:0016747 Catalysis of the transfer of an acyl group, other than amino-acyl, from one compound (donor) to another (acceptor).
  • GO:0008080 Catalysis of the transfer of an acetyl group to a nitrogen atom on the acceptor molecule.
  • GO:0051392 Catalysis of the reaction: a cytidine in tRNA + acetyl-CoA + ATP + H2O = ADP + an N4-acetylcytidine in tRNA + CoA + H+ + phosphate.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:1990883 Catalysis of the reaction: a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA + H+.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:1904812 Any rRNA acetylation that is involved in maturation of SSU-rRNA.

Sequence Features

Domain/signature hits from InterPro and related databases.

25 records
Show feature table
Start End DB Term Name
1 189 FunFam G3DSA:3.40.50.11040:FF:000003 tRNA(Met) cytidine acetyltransferase TmcA
369 511 SUPERFAMILY SSF55729 Acyl-CoA N-acyltransferases (Nat)
369 511 InterPro IPR016181 Acyl-CoA N-acyltransferase
13 648 Hamap MF_01886 tRNA(Met) cytidine acetyltransferase TmcA [tmcA].
13 648 InterPro IPR024914 tRNA(Met) cytidine acetyltransferase TmcA
484 555 PANTHER PTHR10925 N-ACETYLTRANSFERASE 10
484 555 InterPro IPR032672 TmcA/NAT10/Kre33
367 478 Pfam PF13718 GNAT acetyltransferase 2
367 478 InterPro IPR000182 GNAT domain
484 531 Pfam PF13718 GNAT acetyltransferase 2
484 531 InterPro IPR000182 GNAT domain
326 529 Gene3D G3DSA:3.40.630.30 -
530 667 Gene3D G3DSA:1.20.120.890 tRNA(Met) cytidine acetyltransferase, tail domain
530 667 InterPro IPR038321 TmcA, C-terminal domain superfamily
195 335 Pfam PF05127 Helicase
195 335 InterPro IPR007807 Helicase domain
326 529 FunFam G3DSA:3.40.630.30:FF:000054 tRNA(Met) cytidine acetyltransferase TmcA
171 281 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
171 281 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
190 325 FunFam G3DSA:3.40.50.300:FF:001011 tRNA(Met) cytidine acetyltransferase TmcA
190 325 Gene3D G3DSA:3.40.50.300 -
190 325 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
2 189 Gene3D G3DSA:3.40.50.11040 -
535 651 Pfam PF17176 tRNA-binding domain
535 651 InterPro IPR033442 tRNA(Met) cytidine acetyltransferase TmcA, tRNA-binding domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A486QV08
AlphaFold — — full sequence — Viewing
ColabFold KP13_03828
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.411

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 12.36 0.648
2 11.57 0.616
3 5.15 0.237
4 4.19 0.17
5 1.36 0.016