Protein profile

KP13_01026

tRNA pseudouridine synthase A

Genome: KpKP13

Gene: truA AHE43446.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GR85
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Amino acids 270
Annotations 7
Features 21
PDB binders 0
Druggability 0.919

Overview

Basic information about this protein and its source genome.

Accession
KP13_01026
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
truA AHE43446.1
Status
annotated
Amino acids
270
Structure source
AlphaFold + ColabFold
EC
5.4.99.12
GO
GO:0003723 Binding to an RNA molecule or a portion thereof. GO:0001522 The intramolecular conversion of uridine to pseudouridine within an RNA molecule. GO:0009451 The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically. GO:0009982 Catalysis of the reaction: a uridine in RNA = a pseudouridine in RNA. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5. GO:0160147 Catalysis of the reaction: uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA. Modifies uridine(38), uridine(39) and/or uridine(40) in tRNA. GO:0031119 The intramolecular conversion of uridine to pseudouridine in a tRNA molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
28.333
Human E-value
2.55e-06
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
68.846
DEG E-value
1.27e-136
Localization
Cytoplasmic
ColabFold pLDDT
96.01

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.919
Structure A0A0H3GR85
Pocket Pocket 17
P2Rank 0.288
Structure A0A0H3GR85
Pocket Pocket 1
ColabFold model
FPocket 0.557 · Pocket 8
P2Rank 0.353 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 209 / 4744 genomes with a hit
Normalized 0.044

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0003723 Binding to an RNA molecule or a portion thereof.
  • GO:0001522 The intramolecular conversion of uridine to pseudouridine within an RNA molecule.
  • GO:0009451 The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
  • GO:0009982 Catalysis of the reaction: a uridine in RNA = a pseudouridine in RNA. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5.
  • GO:0160147 Catalysis of the reaction: uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA. Modifies uridine(38), uridine(39) and/or uridine(40) in tRNA.
  • GO:0031119 The intramolecular conversion of uridine to pseudouridine in a tRNA molecule.

Sequence Features

Domain/signature hits from InterPro and related databases.

21 records
Show feature table
Start End DB Term Name
10 259 PIRSF PIRSF001430 PSU-A
10 259 InterPro IPR001406 Pseudouridine synthase I, TruA
114 251 Gene3D G3DSA:3.30.70.660 -
114 251 InterPro IPR020095 Pseudouridine synthase I, TruA, C-terminal
9 264 SUPERFAMILY SSF55120 Pseudouridine synthase
9 264 InterPro IPR020103 Pseudouridine synthase, catalytic domain superfamily
10 252 Hamap MF_00171 tRNA pseudouridine synthase A [truA].
10 252 InterPro IPR001406 Pseudouridine synthase I, TruA
11 247 NCBIfam TIGR00071 tRNA pseudouridine(38-40) synthase TruA
11 247 InterPro IPR001406 Pseudouridine synthase I, TruA
151 253 Pfam PF01416 tRNA pseudouridine synthase
151 253 InterPro IPR020097 Pseudouridine synthase I, TruA, alpha/beta domain
17 111 Pfam PF01416 tRNA pseudouridine synthase
17 111 InterPro IPR020097 Pseudouridine synthase I, TruA, alpha/beta domain
114 251 FunFam G3DSA:3.30.70.660:FF:000001 tRNA pseudouridine synthase A
8 113 Gene3D G3DSA:3.30.70.580 -
8 113 InterPro IPR020094 Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal
10 252 PANTHER PTHR11142 PSEUDOURIDYLATE SYNTHASE
9 113 FunFam G3DSA:3.30.70.580:FF:000001 tRNA pseudouridine synthase A
14 252 CDD cd02570 PseudoU_synth_EcTruA
14 252 InterPro IPR001406 Pseudouridine synthase I, TruA

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GR85
AlphaFold — — full sequence — Viewing
ColabFold KP13_01026
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
17 0.919

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 4.68 0.204
2 2.58 0.074
3 2.1 0.048
4 1.81 0.034