Protein profile

KP13_04393

ATP-dependent DNA helicase

Genome: KpKP13

Gene: AHE43541.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GW57
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Amino acids 585
Annotations 6
Features 23
PDB binders 0
Druggability 0.538

Overview

Basic information about this protein and its source genome.

Accession
KP13_04393
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE43541.1
Status
annotated
Amino acids
585
Structure source
AlphaFold + ColabFold
GO
GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). GO:0016787 Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc. GO:0006412 The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA or circRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA or circRNA. Translation ends with the release of a polypeptide chain from the ribosome. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0004386 Catalysis of the reaction: ATP + H2O = ADP + phosphate, to drive the unwinding of a DNA or RNA helix.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
91.14

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.538
Structure A0A0H3GW57
Pocket Pocket 31
P2Rank 0.831
Structure A0A0H3GW57
Pocket Pocket 1
ColabFold model
FPocket 0.683 · Pocket 37
P2Rank 0.651 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 125 / 4744 genomes with a hit
Normalized 0.026

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0016787 Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc.
  • GO:0006412 The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA or circRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA or circRNA. Translation ends with the release of a polypeptide chain from the ribosome.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0004386 Catalysis of the reaction: ATP + H2O = ADP + phosphate, to drive the unwinding of a DNA or RNA helix.

Sequence Features

Domain/signature hits from InterPro and related databases.

23 records
Show feature table
Start End DB Term Name
241 386 ProSiteProfiles PS51194 Superfamilies 1 and 2 helicase C-terminal domain profile.
241 386 InterPro IPR001650 Helicase, C-terminal
186 374 Gene3D G3DSA:3.40.50.300 -
186 374 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
186 374 FunFam G3DSA:3.40.50.300:FF:000859 ATP-dependent RNA helicase
3 154 Pfam PF04851 Type III restriction enzyme, res subunit
3 154 InterPro IPR006935 Helicase/UvrB, N-terminal
424 466 SUPERFAMILY SSF57829 Zn-binding ribosomal proteins
424 466 InterPro IPR011332 Zinc-binding ribosomal protein
2 169 FunFam G3DSA:3.40.50.300:FF:000794 ATP-dependent RNA helicase
3 377 PANTHER PTHR47396 TYPE I RESTRICTION ENZYME ECOKI R PROTEIN
50 363 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
50 363 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
1 170 Gene3D G3DSA:3.40.50.300 -
1 170 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
18 171 ProSiteProfiles PS51192 Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile.
18 171 InterPro IPR014001 Helicase superfamily 1/2, ATP-binding domain
1 185 SMART SM00487 ultradead3
1 185 InterPro IPR014001 Helicase superfamily 1/2, ATP-binding domain
262 345 SMART SM00490 helicmild6
262 345 InterPro IPR001650 Helicase, C-terminal
241 343 Pfam PF00271 Helicase conserved C-terminal domain
241 343 InterPro IPR001650 Helicase, C-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GW57
AlphaFold — — full sequence — Viewing
ColabFold KP13_04393
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
27 0.184
40 0.079
30 0.001
44 0.001

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 22.72 0.831
2 8.43 0.383
3 7.51 0.332
4 5.27 0.203
5 4.93 0.183