Protein profile

KP13_04415

PTS system fructose-specific EIIBC component

Genome: KpKP13

Gene: AHE43564.1 fruA Structure source: AlphaFold + ColabFold UniProt A0A0H3GW46
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Amino acids 564
Annotations 7
Features 50
PDB binders 0
Druggability 0.706

Overview

Basic information about this protein and its source genome.

Accession
KP13_04415
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE43564.1 fruA
Status
annotated
Amino acids
564
Structure source
AlphaFold + ColabFold
GO
GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in). GO:0022877 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + fructose(out) = protein histidine + fructose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
45.714
DEG E-value
2.49e-126
Localization
CytoplasmicMembrane
ColabFold pLDDT
87.57

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.706
Structure A0A0H3GW46
Pocket Pocket 4
P2Rank 0.45
Structure A0A0H3GW46
Pocket Pocket 1
ColabFold model
FPocket 0.799 · Pocket 14
P2Rank 0.564 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 161 / 4744 genomes with a hit
Normalized 0.034

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in).
  • GO:0022877 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + fructose(out) = protein histidine + fructose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

50 records
Show feature table
Start End DB Term Name
482 486 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
303 325 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
232 495 Pfam PF02378 Phosphotransferase system, EIIC
232 495 InterPro IPR003352 Phosphotransferase system, EIIC
97 204 Gene3D G3DSA:3.40.50.2300 -
348 367 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
388 406 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
107 190 NCBIfam TIGR00829 fructose PTS transporter subunit IIB
107 190 InterPro IPR003353 Phosphotransferase system, fructose-specific IIB subunit
106 201 ProSiteProfiles PS51099 PTS_EIIB type-2 domain profile.
106 201 InterPro IPR013011 Phosphotransferase system, EIIB component, type 2
229 255 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
412 434 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
225 560 ProSiteProfiles PS51104 PTS_EIIC type-2 domain profile.
225 560 InterPro IPR013014 Phosphotransferase system, EIIC component, type 2
555 564 Coils Coil Coil
302 328 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
487 506 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
236 258 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
368 387 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
106 201 CDD cd05569 PTS_IIB_fructose
106 201 InterPro IPR003353 Phosphotransferase system, fructose-specific IIB subunit
525 547 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
426 450 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
105 206 SUPERFAMILY SSF52794 PTS system IIB component-like
105 206 InterPro IPR036095 PTS system IIB component-like superfamily
271 293 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 560 PANTHER PTHR30505 FRUCTOSE-LIKE PERMEASE
1 228 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
488 510 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
215 551 NCBIfam TIGR01427 fructose-specific PTS transporter subunit EIIC
215 551 InterPro IPR006327 Phosphotransferase system, fructose IIC component
451 461 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
507 517 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
518 550 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
267 290 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
291 301 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
4 88 SUPERFAMILY SSF52794 PTS system IIB component-like
4 88 InterPro IPR036095 PTS system IIB component-like superfamily
464 481 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
551 564 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
100 205 FunFam G3DSA:3.40.50.2300:FF:000014 PTS system fructose-like transporter subunit IIB
108 194 Pfam PF02302 PTS system, Lactose/Cellobiose specific IIB subunit
108 194 InterPro IPR003501 Phosphotransferase system, EIIB component, type 2/3
345 367 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
377 399 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
407 425 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
329 347 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
256 266 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
462 481 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GW46
AlphaFold — — full sequence — Viewing
ColabFold KP13_04415
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.706
15 0.274
1 0.235
17 0.202

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 5.67 0.274
2 4.57 0.196
3 3.08 0.103
4 2.72 0.082
5 2.68 0.079