Protein profile

KP13_31539

putative PTS system beta-glucoside-specific EIIBCA component

Genome: KpKP13

Gene: AHE43586.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3H0S8

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Amino acids 620

Annotations 7

Features 65

PDB binders 1

Druggability 0.654

Overview

Basic information about this protein and its source genome.

Accession: KP13_31539
Assembly: Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene: AHE43586.1
Status: annotated
Amino acids: 620
Structure source: AlphaFold + ColabFold

GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0090589 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + trehalose (out) = protein cysteine + trehalose-6-phosphate (in).

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Human identity (%): 0.0
Gut microbiome off-target: hit
Essential (DEG): N
DEG identity (%): 0.0
Localization: CytoplasmicMembrane
ColabFold pLDDT: 86.69

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.654

Structure A0A0H3H0S8

Pocket Pocket 1

P2Rank 0.81

Structure A0A0H3H0S8

Pocket Pocket 1

ColabFold model

FPocket 0.641 · Pocket 5

P2Rank 0.862 · Pocket 1

Core conservation Accessory gene

Roary accessory

CoreCruncher accessory

Gut microbiome 47 / 4744 genomes with a hit

Normalized 0.01

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
GO:0090589 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + trehalose (out) = protein cysteine + trehalose-6-phosphate (in).
GO:0015771 The directed movement of trehalose into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Trehalose is a disaccharide that consists of two molecules of glucose and is isomeric with sucrose.

Sequence Features

Domain/signature hits from InterPro and related databases.

65 records

Show feature table

Start	End	DB	Term	Name
536	548	ProSitePatterns	PS00371	PTS EIIA domains phosphorylation site signature 1.
536	548	InterPro	IPR001127	Phosphotransferase system, sugar-specific permease EIIA type 1
467	619	Gene3D	G3DSA:2.70.70.10	Glucose Permease (Domain IIA)
467	619	InterPro	IPR011055	Duplicated hybrid motif
146	163	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
474	597	CDD	cd00210	PTS_IIA_glc
452	620	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
309	328	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
410	428	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
283	308	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
490	594	ProSiteProfiles	PS51093	PTS_EIIA type-1 domain profile.
490	594	InterPro	IPR001127	Phosphotransferase system, sugar-specific permease EIIA type 1
125	143	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
19	36	ProSitePatterns	PS01035	PTS EIIB domains cysteine phosphorylation site signature.
19	36	InterPro	IPR018113	Phosphotransferase system EIIB, cysteine phosphorylation site
329	348	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
207	229	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
387	409	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
2	79	FunFam	G3DSA:3.30.1360.60:FF:000001	PTS system glucose-specific IIBC component PtsG
3	618	NCBIfam	TIGR01995	beta-glucoside-specific PTS transporter subunit IIABC
3	618	InterPro	IPR011297	Phosphotransferase system, beta-glucoside-specific IIABC component
249	271	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
361	379	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
474	594	NCBIfam	TIGR00830	glucose PTS transporter subunit IIA
474	594	InterPro	IPR001127	Phosphotransferase system, sugar-specific permease EIIA type 1
380	385	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
358	380	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
4	72	CDD	cd00212	PTS_IIB_glc
4	72	InterPro	IPR018113	Phosphotransferase system EIIB, cysteine phosphorylation site
170	192	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
109	393	Pfam	PF02378	Phosphotransferase system, EIIC
109	393	InterPro	IPR003352	Phosphotransferase system, EIIC
109	131	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
429	451	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
1	102	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
6	77	SUPERFAMILY	SSF55604	Glucose permease domain IIB
6	77	InterPro	IPR036878	Glucose permease domain IIB
386	409	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
429	451	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
8	41	Pfam	PF00367	phosphotransferase system, EIIB
8	41	InterPro	IPR018113	Phosphotransferase system EIIB, cysteine phosphorylation site
194	212	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
175	193	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
473	597	Pfam	PF00358	phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1
473	597	InterPro	IPR001127	Phosphotransferase system, sugar-specific permease EIIA type 1
329	349	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
464	619	FunFam	G3DSA:2.70.70.10:FF:000001	PTS system glucose-specific IIA component
471	617	SUPERFAMILY	SSF51261	Duplicated hybrid motif
471	617	InterPro	IPR011055	Duplicated hybrid motif
103	124	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
234	252	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
286	308	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
4	86	ProSiteProfiles	PS51098	PTS_EIIB type-1 domain profile.
4	86	InterPro	IPR001996	Phosphotransferase system, IIB component, type 1
350	360	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
164	174	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
253	277	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
1	486	PANTHER	PTHR30175	PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN
105	466	ProSiteProfiles	PS51103	PTS_EIIC type-1 domain profile.
105	466	InterPro	IPR013013	Phosphotransferase system, EIIC component, type 1
213	233	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
144	163	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
278	282	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1	76	Gene3D	G3DSA:3.30.1360.60	Glucose permease domain IIB
1	76	InterPro	IPR036878	Glucose permease domain IIB

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

Loading 3D structure...

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold AF_A0A0H3H0S8	AlphaFold	—	—	full sequence	—	Viewing
ColabFold KP13_31539	ColabFold	—	—	full sequence	—	Loaded

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.654
48				0.312
11				0.238

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK	Score	Probability
1	13.52	0.689
2	4.63	0.201
3	3.6	0.134
4	2.57	0.073
5	2.08	0.047

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
39				0.491
37				0.429
38				0.256

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK	Score	Probability
1	18.18	0.817
2	4.8	0.213
3	3.68	0.139
4	3.53	0.13
5	2.38	0.063

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

1 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

No PDB structure with a co-crystallized ligand found for this exact protein.

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Ligand	Source crystal	UniProt (homolog)	MW · LogP · TPSA	Lipinski	PAINS	SMILES
PO3	2MP0	P69783	79.0 Da LogP -1.64 TPSA 63.2	✓ Ro5	✓ Clean	`[O-][P-](=O)[O-]`

Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL bioactivity data found for this exact protein.

Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL hits found through similar proteins.

Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).

No virtual-screening candidates for this protein.

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.