KpKP13 Protein target profile

putative lipid kinase

Accession: KP13_03155

Gene: AHE43629.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GVW9
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Length 298
Pocket druggability (P2Rank · AlphaFold DB model) 0.425
Functional annotation 1 EC 9 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Structure available AlphaFold DB model + ColabFold model

Risks to review

Low human similarity A human hit was flagged, but identity is only 28.205% - a weak match, lower risk than a close homolog. This target also has a predicted binding pocket (P2Rank 0.42) — check the ligand evidence before deprioritizing on off-target risk alone. Microbiome similarity 102 of 4744 screened genomes (2.2%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.42) — check the ligand evidence before deprioritizing on off-target risk alone. Weak predicted binding pocket FPocket selected-pocket score 0.22. Weak second pocket-predictor signal P2Rank predicted binding-site probability 0.42.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
Hit
Human identity (%)
28.205 Lower values reduce human off-target concern.
Human E-value
8.76e-07
Gut microbiome similarity
2.2% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
77.592 Higher values support similarity to known essential genes.
DEG E-value
9.45e-171 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
88.38 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.425
Structure A0A0H3GVW9
Pocket Pocket 1
Druggability (FPocket) 0.221
Structure A0A0H3GVW9
Pocket Pocket 7
ColabFold model
P2Rank 0.52 · Pocket 1
FPocket 0.326 · Pocket 19
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 102 / 4744 genomes with a hit
Prevalence 2.2%

Sequence

Primary amino-acid sequence viewer.

MMSTFPASLLILNGKGANEPQLREAVNLLRDEGIDIHVRVTWEKGDAARFIDEALQLNVETVIAGGGDGTINEVATALVERGGKMALGILPLGTANDFATSVGIPQDLASALKLAIVGRDVPIDIARVNDKTGFINMATGGFGTRITTETPEKLKAALGGVSYLIHGLMRMDTLKPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPEALINDGLLHLRIFTGEELIPALFSTLANPENSPNIVDGVSSWFEITAPHEMTFNLDGEPLSGKTFRMELLPAALRCRLPPDCPLLR

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 9 GO

Subcellular localization

Localization
Cytoplasmic

Enzyme Commission (EC)

1

Gene Ontology (GO)

9
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0001727 Catalysis of the phosphorylation of a simple or complex lipid.
  • GO:0046872 Binding to a metal ion.
  • GO:0008654 The chemical reactions and pathways resulting in the formation of a phospholipid, a lipid containing phosphoric acid as a mono- or diester.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0000287 Binding to a magnesium (Mg) ion.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

25 records
Show feature table
Start End DB Term Name
7 298 Hamap MF_01377 Probable lipid kinase YegS [yegS].
7 298 InterPro IPR022433 Lipid kinase YegS
4 12 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
128 283 Gene3D G3DSA:2.60.200.40 -
8 297 NCBIfam TIGR03702 lipid kinase YegS
8 297 InterPro IPR022433 Lipid kinase YegS
17 298 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 3 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
1 16 Phobius SIGNAL_PEPTIDE Signal peptide region
9 128 Pfam PF00781 Diacylglycerol kinase catalytic domain
9 128 InterPro IPR001206 Diacylglycerol kinase, catalytic domain
3 127 Gene3D G3DSA:3.40.50.10330 -
3 127 InterPro IPR017438 Inorganic polyphosphate/ATP-NAD kinase, N-terminal
7 128 SMART SM00046 dagk_c4a_7
7 128 InterPro IPR001206 Diacylglycerol kinase, catalytic domain
13 16 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
8 295 SUPERFAMILY SSF111331 NAD kinase/diacylglycerol kinase-like
8 295 InterPro IPR016064 NAD kinase/diacylglycerol kinase-like domain superfamily
3 132 ProSiteProfiles PS50146 DAG-kinase catalytic (DAGKc) domain profile.
3 132 InterPro IPR001206 Diacylglycerol kinase, catalytic domain
14 278 PANTHER PTHR12358 SPHINGOSINE KINASE
149 287 Pfam PF19279 YegS C-terminal NAD kinase beta sandwich-like domain
149 287 InterPro IPR045540 YegS/DAGK, C-terminal domain
2 292 NCBIfam TIGR00147 YegS/Rv2252/BmrU family lipid kinase
2 292 InterPro IPR005218 Diacylglycerol/lipid kinase

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.425
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Surrounding area
Pocket 2 P2Rank #2
0.036
Show in viewer
Surrounding area
Pocket 3 P2Rank #3
0.001
Show in viewer
Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #7
0.221
Show in viewer
Surrounding area
Residue sets
UniProt: Active site:270-270 Proton acceptor
UniProt: Binding site:214-214
UniProt: Binding site:217-217
UniProt: Binding site:219-219
UniProt: Binding site:41-41
UniProt: Binding site:67-73
UniProt: Binding site:94-94
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GVW9
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_03155
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GVW9