Protein profile

KP13_03774

Histidine biosynthesis bifunctional protein hisIE

Genome: KpKP13

Gene: AHE43689.1 hisI Structure source: AlphaFold + ColabFold UniProt A0A0H3GVS9
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Amino acids 199
Annotations 5
Features 20
PDB binders 0
Druggability 0.367

Overview

Basic information about this protein and its source genome.

Accession
KP13_03774
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE43689.1 hisI
Status
annotated
Amino acids
199
Structure source
AlphaFold + ColabFold
GO
GO:0000105 The chemical reactions and pathways resulting in the formation of L-histidine, 2-amino-3-(1H-imidazol-4-yl)propanoic acid. GO:0004635 Catalysis of the reaction: 1-(5-phosphonatoribosyl)-5'-AMP + H2O = 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide. GO:0004636 Catalysis of the reaction: 1-(5-phospho-D-ribosyl)-ATP + H2O = 1-(5-phosphonatoribosyl)-5'-AMP + diphosphate + H+. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
85.714
DEG E-value
2.22e-121
Localization
Cytoplasmic
ColabFold pLDDT
96.24

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.367
Structure A0A0H3GVS9
Pocket Pocket 15
P2Rank 0.041
Structure A0A0H3GVS9
Pocket Pocket 1
ColabFold model
FPocket 0.452 · Pocket 10
P2Rank 0.072 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 463 / 4744 genomes with a hit
Normalized 0.098

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0000105 The chemical reactions and pathways resulting in the formation of L-histidine, 2-amino-3-(1H-imidazol-4-yl)propanoic acid.
  • GO:0004635 Catalysis of the reaction: 1-(5-phosphonatoribosyl)-5'-AMP + H2O = 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide.
  • GO:0004636 Catalysis of the reaction: 1-(5-phospho-D-ribosyl)-ATP + H2O = 1-(5-phosphonatoribosyl)-5'-AMP + diphosphate + H+.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.

Sequence Features

Domain/signature hits from InterPro and related databases.

20 records
Show feature table
Start End DB Term Name
4 117 SUPERFAMILY SSF141734 HisI-like
4 117 InterPro IPR038019 Phosphoribosyl-AMP cyclohydrolase domain superfamily
108 194 Hamap MF_01020 Phosphoribosyl-ATP pyrophosphatase [hisE].
108 194 InterPro IPR008179 Phosphoribosyl-ATP pyrophosphohydrolase
4 199 PANTHER PTHR42945 HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN
112 198 Pfam PF01503 Phosphoribosyl-ATP pyrophosphohydrolase
112 198 InterPro IPR021130 Phosphoribosyl-ATP pyrophosphohydrolase-like
111 198 SUPERFAMILY SSF101386 all-alpha NTP pyrophosphatases
4 199 Hamap MF_01019 Histidine biosynthesis bifunctional protein HisIE [hisI].
4 199 InterPro IPR023019 Histidine biosynthesis bifunctional protein HisIE
29 101 Pfam PF01502 Phosphoribosyl-AMP cyclohydrolase
29 101 InterPro IPR002496 Phosphoribosyl-AMP cyclohydrolase domain
2 93 Gene3D G3DSA:3.10.20.810 -
2 93 InterPro IPR038019 Phosphoribosyl-AMP cyclohydrolase domain superfamily
109 199 Gene3D G3DSA:1.10.287.1080 -
111 194 NCBIfam TIGR03188 phosphoribosyl-ATP diphosphatase
111 194 InterPro IPR008179 Phosphoribosyl-ATP pyrophosphohydrolase
109 199 FunFam G3DSA:1.10.287.1080:FF:000002 Histidine biosynthesis bifunctional protein HisIE
111 194 CDD cd11534 NTP-PPase_HisIE_like
1 93 FunFam G3DSA:3.10.20.810:FF:000001 Histidine biosynthesis bifunctional protein HisIE

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GVS9
AlphaFold — — full sequence — Viewing
ColabFold KP13_03774
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
15 0.367

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 1.79 0.033
2 1.21 0.011