KpKP13 Protein target profile

Shikimate transporter

Accession: KP13_04755

Gene: AHE43719.1 shiA 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GQK7
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Length 439
Pocket druggability (P2Rank · AlphaFold DB model) 0.823
Functional annotation 0 EC 4 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Moderate predicted binding pocket FPocket selected-pocket score 0.41. This supports review but is not experimental validation. Second pocket predictor agrees P2Rank predicted binding-site probability 0.82. Review the complete evidence below

Risks to review

Microbiome similarity 52 of 4744 screened genomes (1.1%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.82) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
1.1% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
85.75 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.823
Structure A0A0H3GQK7
Pocket Pocket 1
Druggability (FPocket) 0.411
Structure A0A0H3GQK7
Pocket Pocket 19
ColabFold model
P2Rank 0.979 · Pocket 1
FPocket 0.832 · Pocket 15
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 52 / 4744 genomes with a hit
Prevalence 1.1%

Sequence

Primary amino-acid sequence viewer.

MDSTISVQPGEAPDSLHRARRAAWGSFAGAVVDWYDFLLYGITAALVFNREFFPQIGPAMGTLAAFATFGVGFLFRPLGGIIFGHFGDRLGRKRMLMMTVWMMGIATACIGLLPSFNQIGWWAPVLLVFLRAVQGFAVGGEWGGAALLSVENAPQGKKAFYSSGVQVGYGVGLLLSTGLVSLISSLTSDQQFLSWGWRLPFLFSVVLVLIALWIRNGMAESQEFEAQQSQDNAPQMKKRLPVVEALLRHPGAFLLIIALRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRIYITGALIGTLSGFPFFMALESQSVFWILFFALMLANIAHDMVVCVQQPMFTELFGASYRYSGAGVGYQVASVVGGGFTPFIAAALVTFSGGSWHSVALYLTAGCLLSALTALLMKKHPVD

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

4
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

54 records
Show feature table
Start End DB Term Name
252 276 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
119 138 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
410 414 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
434 439 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
27 48 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
159 183 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
10 435 PANTHER PTHR43045 SHIKIMATE TRANSPORTER
28 417 Pfam PF00083 Sugar (and other) transporter
28 417 InterPro IPR005828 Major facilitator, sugar transporter-like
215 251 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
415 433 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
22 438 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
22 438 InterPro IPR020846 Major facilitator superfamily domain
139 158 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
311 318 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
252 274 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
60 83 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
114 118 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
95 113 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
410 432 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
195 214 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
288 310 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
49 59 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
319 338 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
318 337 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 26 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
19 237 FunFam G3DSA:1.20.1250.20:FF:000001 Dicarboxylate MFS transporter
25 47 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
29 426 NCBIfam TIGR00883 metabolite/H+ symporter
29 426 InterPro IPR004736 MFS transporter, metabolite:H symporter
20 432 CDD cd17369 MFS_ShiA_like
84 94 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
250 438 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
250 438 InterPro IPR036259 MFS transporter superfamily
18 437 SUPERFAMILY SSF103473 MFS general substrate transporter
18 437 InterPro IPR036259 MFS transporter superfamily
384 406 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
62 84 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
289 311 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
128 150 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
339 343 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
184 194 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
344 364 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
365 383 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
277 287 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
195 214 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
342 364 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
133 158 ProSitePatterns PS00217 Sugar transport proteins signature 2.
133 158 InterPro IPR005829 Sugar transporter, conserved site
163 185 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
384 409 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
21 240 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
21 240 InterPro IPR036259 MFS transporter superfamily
96 118 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.823
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Surrounding area
Pocket 2 P2Rank #2
0.552
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Surrounding area
Pocket 3 P2Rank #3
0.317
Likely same site as FPocket 19 1.6 Å 12 shared residues 86% of smaller site
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Surrounding area
Pocket 4 P2Rank #4
0.083
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Surrounding area
Pocket 5 P2Rank #5
0.015
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #19
0.411 Unusual size
Likely same site as P2Rank 3 1.6 Å 12 shared residues 86% of smaller site
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Surrounding area
Pocket 2 FPocket #20
0.246 Unusual size
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GQK7
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_04755
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GQK7