Overview
Basic information about this protein and its source genome.
- Accession
- KP13_31940
- Gene
- AHE43941.1 pfl
- Status
- annotated
- Amino acids
- 764
- Structure source
- AlphaFold + ColabFold
Target profile
Computed evidence for target prioritization.
- Human off-target
- No hit
- Human identity (%)
- 0.0
- Gut microbiome off-target
- hit
- Essential (DEG)
- Y
- DEG identity (%)
- 74.605
- DEG E-value
- 0.0
- Localization
- Cytoplasmic
- ColabFold pLDDT
- 97.14
Selected Druggability evidence
AlphaFold / UniProt modelSelected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.
Sequence
Primary amino-acid sequence viewer.
Functional Annotations
Enzyme classification and Gene Ontology terms linked to this protein.
Enzyme Commission (EC)
1Gene Ontology (GO)
6- GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
- GO:0008861 Catalysis of the reaction: acetyl-CoA + formate = CoA + pyruvate.
- GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
- GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
- GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
- GO:0006006 The chemical reactions and pathways involving glucose, the aldohexose gluco-hexose. D-glucose is dextrorotatory and is sometimes known as dextrose; it is an important source of energy for living organisms and is found free as well as combined in homo- and hetero-oligosaccharides and polysaccharides.
Sequence Features
Domain/signature hits from InterPro and related databases.
Show feature table
| Start | End | DB | Term | Name |
|---|---|---|---|---|
| 9 | 763 | PANTHER | PTHR30191 | FORMATE ACETYLTRANSFERASE |
| 15 | 764 | NCBIfam | TIGR01255 | formate C-acetyltransferase |
| 15 | 764 | InterPro | IPR005949 | Formate acetyltransferase |
| 7 | 764 | FunFam | G3DSA:3.20.70.20:FF:000003 | Formate acetyltransferase |
| 12 | 764 | PIRSF | PIRSF000379 | For_Ac_trans_1 |
| 634 | 745 | Pfam | PF01228 | Glycine radical |
| 634 | 745 | InterPro | IPR001150 | Glycine radical domain |
| 7 | 764 | Gene3D | G3DSA:3.20.70.20 | - |
| 27 | 618 | Pfam | PF02901 | Pyruvate formate lyase-like |
| 27 | 618 | InterPro | IPR004184 | Pyruvate formate lyase domain |
| 734 | 742 | ProSitePatterns | PS00850 | Glycine radical domain signature. |
| 734 | 742 | InterPro | IPR019777 | Formate C-acetyltransferase glycine radical, conserved site |
| 15 | 760 | CDD | cd01678 | PFL1 |
| 15 | 760 | InterPro | IPR005949 | Formate acetyltransferase |
| 636 | 764 | ProSiteProfiles | PS51149 | Glycine radical domain profile. |
| 636 | 764 | InterPro | IPR001150 | Glycine radical domain |
| 8 | 629 | ProSiteProfiles | PS51554 | Pyruvate formate-lyase domain profile. |
| 8 | 629 | InterPro | IPR004184 | Pyruvate formate lyase domain |
| 8 | 762 | SUPERFAMILY | SSF51998 | PFL-like glycyl radical enzymes |
3D Structure
Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.
Loading 3D structure...
Structural evidence
0 + 2Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.
| Entry | Method | Resolution | Chain | Coverage | Links | Status |
|---|---|---|---|---|---|---|
|
AlphaFold
AF_A0A0J2FYK2
|
AlphaFold | — | — | full sequence | — | Viewing |
|
ColabFold
KP13_31940
|
ColabFold | — | — | full sequence | — | Loaded |
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer
Pockets (FPOCKET)
Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).
| FPOCKET | Sticks | Spheres | Surfaces | Druggability | Labels | Zoom | Positions |
|---|---|---|---|---|---|---|---|
| 36 | 0.536 | ||||||
| 4 | 0.242 | ||||||
| 31 | 0.237 |
Pockets (P2RANK)
Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).
| P2RANK | Sticks | Spheres | Surfaces | Score | Probability | Labels | Zoom | Positions |
|---|---|---|---|---|---|---|---|---|
| 1 | 4.03 | 0.161 | ||||||
| 2 | 3.01 | 0.099 | ||||||
| 3 | 2.54 | 0.072 | ||||||
| 4 | 1.51 | 0.022 | ||||||
| 5 | 1.38 | 0.017 |
Pockets (FPOCKET)
Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).
| FPOCKET | Sticks | Spheres | Surfaces | Druggability | Labels | Zoom | Positions |
|---|---|---|---|---|---|---|---|
| 4 | 0.349 | ||||||
| 34 | 0.296 | ||||||
| 27 | 0.22 |
Pockets (P2RANK)
Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).
| P2RANK | Sticks | Spheres | Surfaces | Score | Probability | Labels | Zoom | Positions |
|---|---|---|---|---|---|---|---|---|
| 1 | 4.81 | 0.214 | ||||||
| 2 | 4.06 | 0.163 | ||||||
| 3 | 2.59 | 0.074 | ||||||
| 4 | 2.22 | 0.054 | ||||||
| 5 | 1.95 | 0.041 |
Ligand evidence
Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.
Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.
No PDB structure with a co-crystallized ligand found for this exact protein.
Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.
| Ligand | Source crystal | UniProt (homolog) | MW · LogP · TPSA | Lipinski | PAINS | SMILES |
|---|---|---|---|---|---|---|
| 8X3 | E5Y378 | 126.1 Da LogP -1.13 TPSA 74.6 | ✓ Ro5 | ✓ Clean |
C(CS(=O)(=O)O)O
|
|
| BTL | Q30W70 | 102.2 Da LogP -0.11 TPSA 17.1 | ✓ Ro5 | ✓ Clean |
C[N+](C)(C)CC=O
|
|
| CHT | A0A0M3KL44 | 104.2 Da LogP -0.32 TPSA 20.2 | ✓ Ro5 | ✓ Clean |
C[N+](C)(C)CCO
|
|
| DTL | P09373 | 122.1 Da LogP -2.31 TPSA 80.9 | ✓ Ro5 | ✓ Clean |
C([C@@H]([C@H](CO)O)O)O
|
|
| HYP | A0A031WDE4 | 131.1 Da LogP -1.21 TPSA 69.6 | ✓ Ro5 | ✓ Clean |
C1[C@H](CN[C@@H]1C(=O)O)O
|
|
| MLI | Q30W70 | 102.0 Da LogP -3.12 TPSA 80.3 | ✓ Ro5 | ✓ Clean |
C(C(=O)[O-])C(=O)[O-]
|
|
| PYR | P09373 | 88.1 Da LogP -0.34 TPSA 54.4 | ✓ Ro5 | ✓ Clean |
CC(=O)C(=O)O
|
|
| RP7 | Q30W70 | 113.2 Da LogP -0.15 TPSA 23.5 | ✓ Ro5 | ✓ Clean |
CN1CC=C[C@@H](C1)O
|
Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).
No ChEMBL bioactivity data found for this exact protein.
Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).
No ChEMBL hits found through similar proteins.
Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).
| Ligand | Tanimoto | MW · LogP · TPSA | Lipinski | PAINS | SMILES |
|---|---|---|---|---|---|
| ZINC1732765 | 0.625 | 246.3 Da LogP -2.20 TPSA 108.7 | ✓ Ro5 | ✓ Clean |
O=S(=O)(CCO)CCS(=O)(=O)CCO
|
| ZINC100055463 | 0.615 | 212.2 Da LogP -4.22 TPSA 141.6 | 1 viol. | ✓ Clean |
OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@H](O)CO
|
| ZINC100064885 | 0.615 | 212.2 Da LogP -4.22 TPSA 141.6 | 1 viol. | ✓ Clean |
OC[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
|
| ZINC17780060 | 0.615 | 212.2 Da LogP -4.22 TPSA 141.6 | 1 viol. | ✓ Clean |
OC[C@@H](O)[C@H](O)C(O)[C@H](O)[C@H](O)CO
|
| ZINC17952732 | 0.615 | 212.2 Da LogP -4.22 TPSA 141.6 | 1 viol. | ✓ Clean |
OC[C@H](O)[C@@H](O)C(O)[C@H](O)[C@H](O)CO
|
| ZINC18042331 | 0.615 | 242.2 Da LogP -4.86 TPSA 161.8 | 1 viol. | ✓ Clean |
OC[C@H](O)[C@H](O)[C@H](O)[C@H](O)[C@H](O)[C@H]…
|
| ZINC18120313 | 0.615 | 212.2 Da LogP -4.22 TPSA 141.6 | 1 viol. | ✓ Clean |
OC[C@H](O)[C@H](O)C(O)[C@H](O)[C@H](O)CO
|
| ZINC3979006 | 0.615 | 212.2 Da LogP -4.22 TPSA 141.6 | 1 viol. | ✓ Clean |
OC[C@@H](O)[C@@H](O)C(O)[C@H](O)[C@H](O)CO
|
| ZINC4403103 | 0.615 | 242.2 Da LogP -4.86 TPSA 161.8 | 1 viol. | ✓ Clean |
OC[C@@H](O)[C@H](O)[C@H](O)[C@H](O)[C@H](O)[C@H…
|
| ZINC4403105 | 0.615 | 242.2 Da LogP -4.86 TPSA 161.8 | 1 viol. | ✓ Clean |
OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@H](O)[C@H…
|
| ZINC4403107 | 0.615 | 242.2 Da LogP -4.86 TPSA 161.8 | 1 viol. | ✓ Clean |
OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@H](O)[C@…
|
| ZINC9212412 | 0.615 | 212.2 Da LogP -4.22 TPSA 141.6 | 1 viol. | ✓ Clean |
OC[C@H](O)[C@@H](O)C(O)[C@H](O)[C@@H](O)CO
|
| ZINC1763010 | 0.563 | 218.3 Da LogP -0.46 TPSA 108.7 | ✓ Ro5 | ✓ Clean |
O=S(=O)(O)CCCCS(=O)(=O)O
|
| ZINC5225211 | 0.563 | 204.2 Da LogP -0.85 TPSA 108.7 | ✓ Ro5 | ✓ Clean |
O=S(=O)(O)CCCS(=O)(=O)O
|
| ZINC5113156 | 0.529 | 246.3 Da LogP 0.32 TPSA 108.7 | ✓ Ro5 | ✓ Clean |
O=S(=O)(O)CCCCCCS(=O)(=O)O
|
| ZINC1659810 | 0.524 | 213.3 Da LogP -1.84 TPSA 98.1 | ✓ Ro5 | ✓ Clean |
O=S(=O)(O)CCN(CCO)CCO
|
| ZINC13541521 | 0.515 | 202.2 Da LogP -1.70 TPSA 98.7 | ✓ Ro5 | ✓ Clean |
C[C@H](NC(=O)[C@@H]1C[C@@H](O)CN1)C(=O)O
|
| ZINC115955979 | 0.500 | 214.2 Da LogP -1.10 TPSA 93.1 | ✓ Ro5 | ✓ Clean |
O=S(=O)(O)CCOCCOCCO
|
| ZINC39591801 | 0.500 | 258.3 Da LogP -1.08 TPSA 102.3 | ✓ Ro5 | ✓ Clean |
O=S(=O)(O)CCOCCOCCOCCO
|
| ZINC5113716 | 0.500 | 232.3 Da LogP -2.24 TPSA 108.7 | ✓ Ro5 | ✓ Clean |
O=S(=O)(CCO)CS(=O)(=O)CCO
|
PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.