Protein profile

KP13_01464

Glutamyl-tRNA reductase

Genome: KpKP13

Gene: AHE44002.1 hemA Structure source: AlphaFold + ColabFold UniProt A0A0H3GUC0
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Amino acids 418
Annotations 5
Features 28
PDB binders 0
Druggability 0.515

Overview

Basic information about this protein and its source genome.

Accession
KP13_01464
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE44002.1 hemA
Status
annotated
Amino acids
418
Structure source
AlphaFold + ColabFold
EC
1.2.1.70
GO
GO:0008883 Catalysis of the reaction: (S)-4-amino-5-oxopentanoate + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + H+ + NADPH. GO:0050661 Binding to nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NADP+, or the reduced form, NADPH. GO:0033014 The chemical reactions and pathways leading to the formation of tetrapyrroles, natural pigments containing four pyrrole rings joined by one-carbon units linking position 2 of one pyrrole ring to position 5 of the next. GO:0019353 The chemical reactions and pathways resulting in the formation of protoporphyrinogen IX from other compounds, including glutamate.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
92.105
DEG E-value
0.0
Localization
Cytoplasmic
ColabFold pLDDT
89.61

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.515
Structure A0A0H3GUC0
Pocket Pocket 8
P2Rank 0.364
Structure A0A0H3GUC0
Pocket Pocket 1
ColabFold model
FPocket 0.516 · Pocket 11
P2Rank 0.131 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 147 / 4744 genomes with a hit
Normalized 0.031

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0008883 Catalysis of the reaction: (S)-4-amino-5-oxopentanoate + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + H+ + NADPH.
  • GO:0050661 Binding to nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NADP+, or the reduced form, NADPH.
  • GO:0033014 The chemical reactions and pathways leading to the formation of tetrapyrroles, natural pigments containing four pyrrole rings joined by one-carbon units linking position 2 of one pyrrole ring to position 5 of the next.
  • GO:0019353 The chemical reactions and pathways resulting in the formation of protoporphyrinogen IX from other compounds, including glutamate.

Sequence Features

Domain/signature hits from InterPro and related databases.

28 records
Show feature table
Start End DB Term Name
2 161 FunFam G3DSA:3.30.460.30:FF:000001 Glutamyl-tRNA reductase
172 306 Pfam PF01488 Shikimate / quinate 5-dehydrogenase
172 306 InterPro IPR006151 Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase
99 122 ProSitePatterns PS00747 Glutamyl-tRNA reductase signature.
99 122 InterPro IPR018214 Glutamyl-tRNA reductase, conserved site
6 156 Pfam PF05201 Glutamyl-tRNAGlu reductase, N-terminal domain
6 156 InterPro IPR015895 Glutamyl-tRNA reductase, N-terminal
3 318 CDD cd05213 NAD_bind_Glutamyl_tRNA_reduct
161 312 Gene3D G3DSA:3.40.50.720 -
338 358 Coils Coil Coil
4 418 NCBIfam TIGR01035 glutamyl-tRNA reductase
4 418 InterPro IPR000343 Glutamyl-tRNA reductase
1 158 SUPERFAMILY SSF69742 Glutamyl tRNA-reductase catalytic, N-terminal domain
1 158 InterPro IPR036343 Glutamyl-tRNA reductase, N-terminal domain superfamily
2 418 Hamap MF_00087 Glutamyl-tRNA reductase [hemA].
2 418 InterPro IPR000343 Glutamyl-tRNA reductase
320 415 Pfam PF00745 Glutamyl-tRNAGlu reductase, dimerisation domain
320 415 InterPro IPR015896 Tetrapyrrole biosynthesis, glutamyl-tRNA reductase, dimerisation domain
161 313 FunFam G3DSA:3.40.50.720:FF:000031 Glutamyl-tRNA reductase
1 417 PANTHER PTHR43013 GLUTAMYL-TRNA REDUCTASE
2 160 Gene3D G3DSA:3.30.460.30 -
2 160 InterPro IPR036343 Glutamyl-tRNA reductase, N-terminal domain superfamily
317 417 SUPERFAMILY SSF69075 Glutamyl tRNA-reductase dimerization domain
317 417 InterPro IPR036453 Glutamyl tRNA-reductase dimerization domain superfamily
1 418 PIRSF PIRSF000445 GluTR
1 418 InterPro IPR000343 Glutamyl-tRNA reductase
162 315 SUPERFAMILY SSF51735 NAD(P)-binding Rossmann-fold domains
162 315 InterPro IPR036291 NAD(P)-binding domain superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GUC0
AlphaFold — — full sequence — Viewing
ColabFold KP13_01464
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
8 0.515
31 0.024
3 0.019
24 0.018

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 8.09 0.364
2 3.86 0.122
3 2.35 0.05
4 1.32 0.012