Protein profile

KP13_04675

50S ribosomal protein L35

Genome: KpKP13

Gene: AHE44065.1 rpmI Structure source: AlphaFold + ColabFold UniProt A0A0H3GRB3
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Amino acids 70
Annotations 4
Features 21
PDB binders 0
Druggability 0.477

Overview

Basic information about this protein and its source genome.

Accession
KP13_04675
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE44065.1 rpmI
Status
annotated
Amino acids
70
Structure source
AlphaFold + ColabFold
GO
GO:0003735 The action of a molecule that contributes to the structural integrity of the ribosome. GO:0005840 An intracellular organelle, about 200 A in diameter, consisting of RNA and protein. It is the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). It consists of two subunits, one large and one small, each containing only protein and RNA. Both the ribosome and its subunits are characterized by their sedimentation coefficients, expressed in Svedberg units (symbol: S). Hence, the prokaryotic ribosome (70S) comprises a large (50S) subunit and a small (30S) subunit, while the eukaryotic ribosome (80S) comprises a large (60S) subunit and a small (40S) subunit. Two sites on the ribosomal large subunit are involved in translation, namely the aminoacyl site (A site) and peptidyl site (P site). Ribosomes from prokaryotes, eukaryotes, mitochondria, and chloroplasts have characteristically distinct ribosomal proteins. GO:0006412 The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA or circRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA or circRNA. Translation ends with the release of a polypeptide chain from the ribosome. GO:0022625 The large subunit of a ribosome located in the cytosol.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
100.0
DEG E-value
1.41e-41
Localization
Cytoplasmic
ColabFold pLDDT
89.76

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.477
Structure A0A0H3GRB3
Pocket Pocket 4
P2Rank
Structure A0A0H3GRB3
Pocket No pockets
ColabFold model
FPocket 0.812 · Pocket 2
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 825 / 4744 genomes with a hit
Normalized 0.174

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0003735 The action of a molecule that contributes to the structural integrity of the ribosome.
  • GO:0005840 An intracellular organelle, about 200 A in diameter, consisting of RNA and protein. It is the site of protein biosynthesis resulting from translation of messenger RNA (mRNA). It consists of two subunits, one large and one small, each containing only protein and RNA. Both the ribosome and its subunits are characterized by their sedimentation coefficients, expressed in Svedberg units (symbol: S). Hence, the prokaryotic ribosome (70S) comprises a large (50S) subunit and a small (30S) subunit, while the eukaryotic ribosome (80S) comprises a large (60S) subunit and a small (40S) subunit. Two sites on the ribosomal large subunit are involved in translation, namely the aminoacyl site (A site) and peptidyl site (P site). Ribosomes from prokaryotes, eukaryotes, mitochondria, and chloroplasts have characteristically distinct ribosomal proteins.
  • GO:0006412 The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA or circRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA or circRNA. Translation ends with the release of a polypeptide chain from the ribosome.
  • GO:0022625 The large subunit of a ribosome located in the cytosol.

Sequence Features

Domain/signature hits from InterPro and related databases.

21 records
Show feature table
Start End DB Term Name
6 69 FunFam G3DSA:4.10.410.60:FF:000001 50S ribosomal protein L35
6 69 Hamap MF_00514 50S ribosomal protein L35 [rpmI].
6 69 InterPro IPR001706 Ribosomal protein L35, non-mitochondrial
6 69 Gene3D G3DSA:4.10.410.60 -
6 69 InterPro IPR037229 Ribosomal protein L35 superfamily
26 40 PRINTS PR00064 Ribosomal protein L35 signature
26 40 InterPro IPR001706 Ribosomal protein L35, non-mitochondrial
8 25 PRINTS PR00064 Ribosomal protein L35 signature
8 25 InterPro IPR001706 Ribosomal protein L35, non-mitochondrial
41 59 PRINTS PR00064 Ribosomal protein L35 signature
41 59 InterPro IPR001706 Ribosomal protein L35, non-mitochondrial
7 69 NCBIfam TIGR00001 50S ribosomal protein L35
7 69 InterPro IPR001706 Ribosomal protein L35, non-mitochondrial
10 36 ProSitePatterns PS00936 Ribosomal protein L35 signature.
10 36 InterPro IPR018265 Ribosomal protein L35, conserved site
7 65 Pfam PF01632 Ribosomal protein L35
7 65 InterPro IPR021137 Ribosomal protein L35
7 69 SUPERFAMILY SSF143034 L35p-like
7 69 InterPro IPR037229 Ribosomal protein L35 superfamily
5 70 PANTHER PTHR33343 54S RIBOSOMAL PROTEIN BL35M
5 70 InterPro IPR001706 Ribosomal protein L35, non-mitochondrial

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GRB3
AlphaFold — — full sequence — Viewing
ColabFold KP13_04675
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
5 0.086
2 0.027
3 0.017
1 0.001