KpKP13 Protein target profile

Putative 3-hydroxyphenylpropionic acid transporter

Accession: KP13_04606

Gene: AHE44134.1 mhpT 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GUS7
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Length 398
Pocket druggability (P2Rank · AlphaFold DB model) 0.792
Functional annotation 0 EC 5 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.79, FPocket 0.90). Use the 3D pocket comparison to verify whether they identify the same site. Strong predicted binding pocket FPocket selected-pocket score 0.90. This is a computational pocket signal, not binding validation. Second pocket predictor agrees P2Rank predicted binding-site probability 0.79. Structure available AlphaFold DB model + ColabFold model

Risks to review

Human similarity Top human hit identity: 36.585%. This target also has a predicted binding pocket (P2Rank 0.79) — check the ligand evidence before deprioritizing on off-target risk alone. Microbiome similarity 23 of 4744 screened genomes (0.5%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.79) — check the ligand evidence before deprioritizing on off-target risk alone. Mixed conservation Core/accessory calls are not fully concordant.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
Hit
Human identity (%)
36.585 Lower values reduce human off-target concern.
Human E-value
8.24e-07
Gut microbiome similarity
0.5% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
51.053 Higher values support similarity to known essential genes.
DEG E-value
1.84e-84 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
89.28 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.792
Structure A0A0H3GUS7
Pocket Pocket 1
Druggability (FPocket) 0.896
Structure A0A0H3GUS7
Pocket Pocket 19
ColabFold model
P2Rank 0.81 · Pocket 1
FPocket 0.577 · Pocket 23
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 23 / 4744 genomes with a hit
Prevalence 0.5%

Sequence

Primary amino-acid sequence viewer.

MTKITTATPSRLVVTIGLCFMVALMEGLDLQAAGIAAVGMAQAFALDKMQMGWIFSAGILGLLPGALVGGMLADRHGRKRILLGSVLLFGLFSLATALAWSFPTLLLARLLTGVGLGAALPNLIALTSEAAGSRFRGRAVSLMYCGVPIGAALAAALGFSGLAAAWQIIFWIGGVVPLLLIPLLMRWLPESQAFQRAEASVPLRTLFAPGQAAATLLLWLGYFFTLLVVYMLINWLPMLLVGQGFRASQAAGVMFSLQIGAACGTLLLGALMDKLTPLRMSLLIYSGILASLLALGSASSLTGMLLAGFVAGLFATGGQSVLYALAPLFYPAAIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGIVLAGVAVFWLMHRQQRAAMV

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

5
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0046943 Enables the transfer of carboxylic acids from one side of a membrane to the other. Carboxylic acids are organic acids containing one or more carboxyl (COOH) groups or anions (COO-).

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

51 records
Show feature table
Start End DB Term Name
303 325 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
160 164 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
328 349 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
81 100 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
250 272 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
20 297 Pfam PF07690 Major Facilitator Superfamily
20 297 InterPro IPR011701 Major facilitator superfamily
128 138 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
10 206 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
10 206 InterPro IPR036259 MFS transporter superfamily
213 235 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
50 72 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
101 105 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
390 398 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
186 205 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
272 282 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 11 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
140 162 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
139 159 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
81 100 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
253 271 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
16 392 CDD cd17365 MFS_PcaK_like
234 252 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
211 397 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
211 397 InterPro IPR036259 MFS transporter superfamily
14 395 PANTHER PTHR23508 CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG
42 50 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
350 360 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
166 188 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 393 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
15 393 InterPro IPR020846 Major facilitator superfamily domain
165 185 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
283 316 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
105 127 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
206 233 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
106 127 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
9 394 SUPERFAMILY SSF103473 MFS general substrate transporter
9 394 InterPro IPR036259 MFS transporter superfamily
281 298 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
332 351 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
317 327 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
361 389 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 41 Phobius SIGNAL_PEPTIDE Signal peptide region
70 80 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
12 23 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
24 41 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
366 388 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
51 69 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
111 136 ProSitePatterns PS00217 Sugar transport proteins signature 2.
111 136 InterPro IPR005829 Sugar transporter, conserved site

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.792
Likely same site as FPocket 19 5.4 Å 21 shared residues 78% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.209
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Surrounding area
Pocket 3 P2Rank #3
0.203
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Surrounding area
Pocket 4 P2Rank #4
0.007
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Surrounding area
Pocket 5 P2Rank #5
0.006
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #19
0.896 Unusual size
Likely same site as P2Rank 1 5.4 Å 21 shared residues 78% of smaller site
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GUS7
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_04606
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GUS7