Protein profile

KP13_05061

putative sensor histidine protein kinase

Genome: KpKP13

Gene: AHE44187.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GPB5
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Amino acids 353
Annotations 5
Features 42
PDB binders 4
Druggability 0.825

Overview

Basic information about this protein and its source genome.

Accession
KP13_05061
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE44187.1
Status
annotated
Amino acids
353
Structure source
AlphaFold + ColabFold
GO
GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor). GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
85.29

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.825
Structure A0A0H3GPB5
Pocket Pocket 1
P2Rank 0.937
Structure A0A0H3GPB5
Pocket Pocket 1
ColabFold model
FPocket 0.104 · Pocket 4
P2Rank 0.947 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 18 / 4744 genomes with a hit
Normalized 0.004

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

Sequence Features

Domain/signature hits from InterPro and related databases.

42 records
Show feature table
Start End DB Term Name
81 135 SMART SM00304 HAMP_11
81 135 InterPro IPR003660 HAMP domain
249 351 CDD cd00075 HATPase
245 352 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
245 352 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
58 77 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
143 353 ProSiteProfiles PS50109 Histidine kinase domain profile.
143 353 InterPro IPR005467 Histidine kinase domain
12 34 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
47 133 Gene3D G3DSA:6.10.340.10 -
81 135 ProSiteProfiles PS50885 HAMP domain profile.
134 195 CDD cd00082 HisKA
134 195 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
315 333 PRINTS PR00344 Bacterial sensor protein C-terminal signature
315 333 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
280 294 PRINTS PR00344 Bacterial sensor protein C-terminal signature
280 294 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
339 352 PRINTS PR00344 Bacterial sensor protein C-terminal signature
339 352 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
298 308 PRINTS PR00344 Bacterial sensor protein C-terminal signature
298 308 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
39 57 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
116 136 Coils Coil Coil
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
54 76 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
244 353 SMART SM00387 HKATPase_4
244 353 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
82 132 SUPERFAMILY SSF158472 HAMP domain-like
78 353 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
60 349 PANTHER PTHR45528 SENSOR HISTIDINE KINASE CPXA
86 130 CDD cd06225 HAMP
119 195 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
119 195 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
206 353 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
206 353 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
134 197 Gene3D G3DSA:1.10.287.130 -
12 38 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
206 353 Gene3D G3DSA:3.30.565.10 -
140 198 Pfam PF00512 His Kinase A (phospho-acceptor) domain
140 198 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
136 200 SMART SM00388 HisKA_10
136 200 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GPB5
AlphaFold — — full sequence — Viewing
ColabFold KP13_05061
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.825

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 22.6 0.882

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

54 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
EMC
3A0W
Q9X180 229.7 Da LogP 0.97 TPSA 0.0 ✓ Ro5 ✓ Clean CC[Hg+]
EMT
3A0W
Q9X180 382.8 Da LogP 2.91 TPSA 37.3 ✓ Ro5 ✓ Clean CC[Hg]Sc1ccccc1C(=O)O
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.