Protein profile

KP13_31993

glucan biosynthesis protein D

Genome: KpKP13

Gene: AHE44319.1 mdoD Structure source: AlphaFold + ColabFold UniProt A0A0H3GP27
Export view
Amino acids 614
Annotations 7
Features 26
PDB binders 0
Druggability 0.945

Overview

Basic information about this protein and its source genome.

Accession
KP13_31993
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE44319.1 mdoD
Status
annotated
Amino acids
614
Structure source
AlphaFold + ColabFold
GO
GO:0030246 Binding to a carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates. GO:0042597 The region between the inner (cytoplasmic) and outer membrane (Gram-negative Bacteria) or cytoplasmic membrane and cell wall (Fungi and Gram-positive Bacteria). GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. GO:0016051 The chemical reactions and pathways resulting in the formation of carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y. GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Periplasmic
ColabFold pLDDT
85.0

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.945
Structure A0A0H3GP27
Pocket Pocket 38
P2Rank 0.868
Structure A0A0H3GP27
Pocket Pocket 1
ColabFold model
FPocket 0.599 · Pocket 44
P2Rank 0.621 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 74 / 4744 genomes with a hit
Normalized 0.016

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0030246 Binding to a carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates.
  • GO:0042597 The region between the inner (cytoplasmic) and outer membrane (Gram-negative Bacteria) or cytoplasmic membrane and cell wall (Fungi and Gram-positive Bacteria).
  • GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0016051 The chemical reactions and pathways resulting in the formation of carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
  • GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.
  • GO:0051274 The chemical reactions and pathways resulting in the formation of beta-glucans.

Sequence Features

Domain/signature hits from InterPro and related databases.

26 records
Show feature table
Start End DB Term Name
33 73 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
14 32 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
73 95 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
64 601 PIRSF PIRSF006281 MdoG
64 601 InterPro IPR014438 Glucan biosynthesis protein MdoG/MdoD
64 607 Hamap MF_01068 Glucans biosynthesis protein D [mdoD].
64 607 InterPro IPR023724 Glucan biosynthesis, MdoD
108 597 Pfam PF04349 Periplasmic glucan biosynthesis protein, MdoG
108 597 InterPro IPR007444 Glucan biosynthesis, periplasmic, MdoG C-terminal
64 602 PANTHER PTHR30504 GLUCANS BIOSYNTHESIS PROTEIN
64 602 InterPro IPR014438 Glucan biosynthesis protein MdoG/MdoD
15 37 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 95 ProSiteProfiles PS51318 Twin arginine translocation (Tat) signal profile.
1 95 InterPro IPR006311 Twin-arginine translocation pathway, signal sequence
487 606 FunFam G3DSA:2.60.40.10:FF:000379 Glucans biosynthesis protein D
95 614 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
107 484 Gene3D G3DSA:2.70.98.10 -
107 484 InterPro IPR014718 Glycoside hydrolase-type carbohydrate-binding
486 600 SUPERFAMILY SSF81296 E set domains
486 600 InterPro IPR014756 Immunoglobulin E-set
108 485 SUPERFAMILY SSF74650 Galactose mutarotase-like
108 485 InterPro IPR011013 Galactose mutarotase-like domain superfamily
1 13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
487 607 Gene3D G3DSA:2.60.40.10 Immunoglobulins
487 607 InterPro IPR013783 Immunoglobulin-like fold
74 94 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GP27
AlphaFold — — full sequence — Viewing
ColabFold KP13_31993
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
38 0.945
24 0.932
21 0.244

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 9.33 0.501
2 6.27 0.314
3 4.05 0.162
4 2.62 0.076
5 2.41 0.065