Protein profile

KP13_05537

Fructose-like phosphotransferase enzyme IIB component

Genome: KpKP13

Gene: AHE44543.1 Structure source: AlphaFold + ColabFold UniProt A6T9A2
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Amino acids 103
Annotations 3
Features 13
PDB binders 0
Druggability 0.378

Overview

Basic information about this protein and its source genome.

Accession
KP13_05537
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE44543.1
Status
annotated
Amino acids
103
Structure source
AlphaFold + ColabFold
GO
GO:0022877 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + fructose(out) = protein histidine + fructose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
44.33
DEG E-value
2.13e-21
Localization
Cytoplasmic
ColabFold pLDDT
94.72

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.378
Structure A6T9A2
Pocket Pocket 1
P2Rank
Structure A6T9A2
Pocket No pockets
ColabFold model
FPocket 0.342 · Pocket 3
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 174 / 4744 genomes with a hit
Normalized 0.037

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3
  • GO:0022877 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + fructose(out) = protein histidine + fructose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

13 records
Show feature table
Start End DB Term Name
3 96 CDD cd05569 PTS_IIB_fructose
3 96 InterPro IPR003353 Phosphotransferase system, fructose-specific IIB subunit
2 97 PANTHER PTHR30505 FRUCTOSE-LIKE PERMEASE
4 88 NCBIfam TIGR00829 fructose PTS transporter subunit IIB
4 88 InterPro IPR003353 Phosphotransferase system, fructose-specific IIB subunit
5 96 Pfam PF02302 PTS system, Lactose/Cellobiose specific IIB subunit
5 96 InterPro IPR003501 Phosphotransferase system, EIIB component, type 2/3
1 103 Gene3D G3DSA:3.40.50.2300 -
2 97 SUPERFAMILY SSF52794 PTS system IIB component-like
2 97 InterPro IPR036095 PTS system IIB component-like superfamily
1 99 ProSiteProfiles PS51099 PTS_EIIB type-2 domain profile.
1 99 InterPro IPR013011 Phosphotransferase system, EIIB component, type 2
1 103 FunFam G3DSA:3.40.50.2300:FF:000014 PTS system fructose-like transporter subunit IIB

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A6T9A2
AlphaFold — — full sequence — Viewing
ColabFold KP13_05537
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.378