KpKP13 Protein target profile

Inner membrane transport protein

Accession: KP13_01165

Gene: AHE44746.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GT16
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Length 407
Pocket druggability (P2Rank · AlphaFold DB model) 0.968
Functional annotation 0 EC 4 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.97, FPocket 0.75). Use the 3D pocket comparison to verify whether they identify the same site. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Strong predicted binding pocket FPocket selected-pocket score 0.75. This is a computational pocket signal, not binding validation. Second pocket predictor agrees P2Rank predicted binding-site probability 0.97. Review the complete evidence below

Risks to review

Human similarity Top human hit identity: 30%. This target also has a predicted binding pocket (P2Rank 0.97) — check the ligand evidence before deprioritizing on off-target risk alone. Microbiome similarity 119 of 4744 screened genomes (2.5%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.97) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
Hit
Human identity (%)
30.0 Lower values reduce human off-target concern.
Human E-value
3.32e-07
Gut microbiome similarity
2.5% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
91.12 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.968
Structure A0A0H3GT16
Pocket Pocket 1
Druggability (FPocket) 0.751
Structure A0A0H3GT16
Pocket Pocket 8
ColabFold model
P2Rank 0.981 · Pocket 1
FPocket 0.87 · Pocket 15
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 119 / 4744 genomes with a hit
Prevalence 2.5%

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GT16

Sequence

Primary amino-acid sequence viewer.

MPAGEVSVAGASRPDPFIKRGTPQFMRVTLALFSAGLATFALLYCVQPILPVLSNEFGVSPASSSISLSISTAMLAVGLLFTGPLSDAIGRKPVMVTALLLAACCSLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAFSMGLYISGNSIGGMSGRLLTGVFTDFFGWRVALAAISGFALAAAIMFWRILPESRHFRPTSLRPKTLLINFRLHWRDRGLPLLFVEGFLLMGAFVTLFNYIGYRLMMSPWSLSQAVVGLLSVAYLTGTWSSPKAGAMTVRFGRGPVMLGFTAVMLCGLLLTLFSSLWLIFIGMLLFSAGFFAAHSVASSWIGPRARRARGQASSLYLFSYYLGSSLAGTLGGVFWHHYGWNGVGGFIALLLLAALLTGTCLHQRLK

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

4
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

49 records
Show feature table
Start End DB Term Name
141 146 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
319 341 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
277 296 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
253 257 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
93 115 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
167 177 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
380 402 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
383 402 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
403 407 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
148 170 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
344 354 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
180 202 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
29 388 CDD cd17324 MFS_NepI_like
355 377 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
114 118 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
292 314 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
119 141 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
32 252 Pfam PF07690 Major Facilitator Superfamily
32 252 InterPro IPR011701 Major facilitator superfamily
354 376 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
378 382 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
28 50 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 27 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
258 276 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
62 82 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
119 140 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
28 50 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
230 252 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
297 314 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
147 166 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
178 199 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
65 86 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
82 98 ProSitePatterns PS00216 Sugar transport proteins signature 1.
82 98 InterPro IPR005829 Sugar transporter, conserved site
320 343 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
94 113 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
257 279 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
51 61 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
315 319 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
200 230 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
21 400 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
21 400 InterPro IPR036259 MFS transporter superfamily
83 93 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
24 407 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
24 407 InterPro IPR020846 Major facilitator superfamily domain
13 406 PANTHER PTHR43271 BLL2771 PROTEIN
19 403 SUPERFAMILY SSF103473 MFS general substrate transporter
19 403 InterPro IPR036259 MFS transporter superfamily
231 252 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.968
Likely same site as FPocket 8 3.4 Å 27 shared residues 93% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.378
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Surrounding area
Pocket 3 P2Rank #3
0.315
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Surrounding area
Pocket 4 P2Rank #4
0.048
Likely same site as FPocket 13 2.5 Å 9 shared residues 90% of smaller site
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Surrounding area
Pocket 5 P2Rank #5
0.039
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #8
0.751 Unusual size
Likely same site as P2Rank 1 3.4 Å 27 shared residues 93% of smaller site
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Surrounding area
Pocket 2 FPocket #4
0.495
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Surrounding area
Pocket 3 FPocket #13
0.334
Likely same site as P2Rank 4 2.5 Å 9 shared residues 90% of smaller site
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GT16
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_01165
ColabFold — — full sequence — Loaded