Protein profile

KP13_05011

PTS system maltose- and glucose-specific EIICB component

Genome: KpKP13

Gene: malX AHE44814.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GWR7
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Amino acids 530
Annotations 7
Features 53
PDB binders 0
Druggability 0.839

Overview

Basic information about this protein and its source genome.

Accession
KP13_05011
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
malX AHE44814.1
Status
annotated
Amino acids
530
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0090564 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + glucose(out) = protein cysteine + glucose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
89.53

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.839
Structure A0A0H3GWR7
Pocket Pocket 5
P2Rank 0.92
Structure A0A0H3GWR7
Pocket Pocket 1
ColabFold model
FPocket 0.853 · Pocket 2
P2Rank 0.904 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 109 / 4744 genomes with a hit
Normalized 0.023

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090564 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + glucose(out) = protein cysteine + glucose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:1904659 The process in which D-glucose is transported across a membrane.

Sequence Features

Domain/signature hits from InterPro and related databases.

53 records
Show feature table
Start End DB Term Name
425 530 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
138 160 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
138 161 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
43 61 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
96 118 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
324 347 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
448 525 FunFam G3DSA:3.30.1360.60:FF:000001 PTS system glucose-specific IIBC component PtsG
449 530 ProSiteProfiles PS51098 PTS_EIIB type-1 domain profile.
449 530 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
464 481 ProSitePatterns PS01035 PTS EIIB domains cysteine phosphorylation site signature.
464 481 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
375 380 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
8 525 NCBIfam TIGR02004 maltose/glucose-specific PTS transporter subunit IIBC
8 525 InterPro IPR011301 PTS system, maltose/glucose-specific IIBC component
400 404 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
397 419 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
21 42 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
58 89 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
162 181 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
90 95 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
381 399 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
405 424 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
323 345 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
9 431 ProSiteProfiles PS51103 PTS_EIIC type-1 domain profile.
9 431 InterPro IPR013013 Phosphotransferase system, EIIC component, type 1
119 137 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
182 204 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
10 527 PANTHER PTHR30009 CYTOCHROME C-TYPE SYNTHESIS PROTEIN AND PTS TRANSMEMBRANE COMPONENT
454 526 SUPERFAMILY SSF55604 Glucose permease domain IIB
454 526 InterPro IPR036878 Glucose permease domain IIB
353 374 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
70 372 NCBIfam TIGR00852 maltose/glucose-specific PTS transporter subunit IIC
70 372 InterPro IPR004719 Phosphotransferase system, maltose/glucose-specific subfamily IIC component
348 352 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
352 374 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 20 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
62 89 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
450 527 Gene3D G3DSA:3.30.1360.60 Glucose permease domain IIB
450 527 InterPro IPR036878 Glucose permease domain IIB
295 312 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
206 294 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
19 361 Pfam PF02378 Phosphotransferase system, EIIC
19 361 InterPro IPR003352 Phosphotransferase system, EIIC
420 511 NCBIfam TIGR00826 glucose PTS transporter subunit EIIB
420 511 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
313 323 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
452 525 CDD cd00212 PTS_IIB_glc
452 525 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
182 205 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
454 486 Pfam PF00367 phosphotransferase system, EIIB
454 486 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
96 118 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GWR7
AlphaFold — — full sequence — Viewing
ColabFold KP13_05011
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
5 0.839
10 0.439

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 17.98 0.812
2 4.87 0.218
3 2.28 0.057
4 1.55 0.023