KpKP13 Protein target profile

Excinuclease cho

Accession: KP13_05393

Gene: AHE45038.1 cho 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GN81
Length 287
Pocket druggability (P2Rank · AlphaFold DB model) 0.056
Functional annotation 0 EC 5 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
1.6% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
75.175 Higher values support similarity to known essential genes.
DEG E-value
7.88e-160 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
89.97 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.056
Structure A0A0H3GN81
Pocket Pocket 1
Druggability (FPocket) 0.419
Structure A0A0H3GN81
Pocket Pocket 6
ColabFold model
P2Rank 0.033 · Pocket 1
FPocket 0.456 · Pocket 3
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 75 / 4744 genomes with a hit
Prevalence 1.6%

Sequence

Primary amino-acid sequence viewer.

MARKQSAPRLEFEAAAIYEYPEHLRPWLEALPKLPGVYQFHGDSDTMPLYIGKSVNLRSRVLSHLRTPEEAAMLRQSRRITWQRTAGELGALLLEARLIKEQQPLFNKRLRRNKQLCAWLLADDRPQIVYAREVDFSHQQHLYGLFANRRAALQMLQSLADEQRLCYGLLGLEPLSRGRACFRSALGRCAGACCGKESVEAHRERLLAQMSRLQLVCWPWAGPVALEERGPDMTQYHVIHNWLWLGAVESLDQAAELTRLPAGFDQDGYKILCKPLLSGDYPLHPLG

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Subcellular localization

Localization
Cytoplasmic

Gene Ontology (GO)

5
  • GO:0006289 A DNA repair process in which a small region of the strand surrounding the damage is removed from the DNA helix as an oligonucleotide. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. Nucleotide excision repair recognizes a wide range of substrates, including damage caused by UV irradiation (pyrimidine dimers and 6-4 photoproducts) and chemicals (intrastrand cross-links and bulky adducts).
  • GO:0009380 Any of the protein complexes formed by the UvrABC excinuclease system, which carries out nucleotide excision repair. Three different complexes are formed by the 3 proteins as they proceed through the excision repair process. First a complex consisting of two A subunits and two B subunits bind DNA and unwind it around the damaged site. Then, the A subunits disassociate leaving behind a stable complex between B subunits and DNA. Now, subunit C binds to this B+DNA complex and causes subunit B to nick the DNA on one side of the complex while subunit C nicks the DNA on the other side of the complex. DNA polymerase I and DNA ligase can then repair the resulting gap.
  • GO:0004519 Catalysis of the cleavage of ester linkages within nucleic acids by creating internal breaks.
  • GO:0016787 Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc.
  • GO:0009432 An error-prone process for repairing damaged microbial DNA.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

12 records
Show feature table
Start End DB Term Name
34 112 SMART SM00465 uri_9
34 112 InterPro IPR000305 GIY-YIG endonuclease
21 116 FunFam G3DSA:3.40.1440.10:FF:000004 UV-repair endonuclease Cho
33 108 ProSiteProfiles PS50164 GIY-YIG domain profile.
33 108 InterPro IPR000305 GIY-YIG endonuclease
31 108 CDD cd10434 GIY-YIG_UvrC_Cho
31 108 InterPro IPR047296 UvrC/Cho-like, GIY-YIG domain
21 116 Gene3D G3DSA:3.40.1440.10 -
21 116 InterPro IPR035901 GIY-YIG endonuclease superfamily
18 256 PANTHER PTHR30562 UVRC/OXIDOREDUCTASE
34 111 SUPERFAMILY SSF82771 GIY-YIG endonuclease
34 111 InterPro IPR035901 GIY-YIG endonuclease superfamily

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.056
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Surrounding area
Pocket 2 P2Rank #2
0.014
Likely same site as FPocket 1 4.7 Å 8 shared residues 100% of smaller site
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Surrounding area
Pocket 3 P2Rank #3
0.01
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Surrounding area
Pocket 4 P2Rank #4
0.006
Likely same site as FPocket 6 1.4 Å 7 shared residues 100% of smaller site
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Surrounding area
Pocket 5 P2Rank #5
0.005
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #6
0.419
Likely same site as P2Rank 4 1.4 Å 7 shared residues 100% of smaller site
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Surrounding area
Pocket 2 FPocket #1
0.236 Unusual size
Likely same site as P2Rank 2 4.7 Å 8 shared residues 100% of smaller site
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GN81
AlphaFold DB full sequence Viewing
ColabFold KP13_05393
ColabFold full sequence Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.