KpKP13 Protein target profile

Inner membrane transport protein

Accession: KP13_05610

Gene: AHE45099.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GLM8
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Length 394
Pocket druggability (P2Rank · AlphaFold DB model) 0.934
Functional annotation 0 EC 3 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Moderate predicted binding pocket FPocket selected-pocket score 0.60. This supports review but is not experimental validation. Second pocket predictor agrees P2Rank predicted binding-site probability 0.93. Review the complete evidence below

Risks to review

Microbiome similarity 63 of 4744 screened genomes (1.3%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.93) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
1.3% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
77.806 Higher values support similarity to known essential genes.
DEG E-value
0.0 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
88.27 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.934
Structure A0A0H3GLM8
Pocket Pocket 1
Druggability (FPocket) 0.605
Structure A0A0H3GLM8
Pocket Pocket 16
ColabFold model
P2Rank 0.914 · Pocket 1
FPocket 0.704 · Pocket 25
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 63 / 4744 genomes with a hit
Prevalence 1.3%

Sequence

Primary amino-acid sequence viewer.

MSSVISSTGRRPALLIAGILLIATTLRVVFTGAAPLLDAIRSDYGLTTAQTGLLTTLPLLAFGLVSPLAAGVARRFGMERSLLLAMLLICAGIALRSLPSAALLFIGTAVIGCGIALGNVLLPGLIKRDFSQHVARMTGAYSLTMGGAAALGSALVVPVAMAGFGWRGALLLLMVFPLLALLSWLPQSRRRAETPLTGSGAMHNRGIWRSALAWQVTLFLGINSLVYYVIIGWLPSILQSMGYSEAQAGSLHGLLQLATAAPGLAIPLILHRLRDQRGIAVLVALMCAISAAGLWLLPELAIGWTLLFGFGSGATMILGLTFIGLRASSAHQAAALSGMAQSVGYLLAACGPPLMGRIHDANGDWHIPLLAVALISLVMAVCGALAGRDREIHP

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

3
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

48 records
Show feature table
Start End DB Term Name
388 394 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
278 297 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
138 160 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
186 211 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
11 391 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
11 391 InterPro IPR020846 Major facilitator superfamily domain
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 368 NCBIfam TIGR00896 CynX/NimT family MFS transporter
15 368 InterPro IPR004747 Cyanate transport protein CynX-like
104 126 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 37 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
50 72 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
235 253 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
333 355 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
302 323 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
272 277 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
365 387 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
164 186 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
127 137 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
138 160 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
212 234 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
6 384 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
6 384 InterPro IPR036259 MFS transporter superfamily
103 125 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
356 366 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
71 81 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 392 PANTHER PTHR23523 -
82 98 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
367 387 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
38 48 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
297 301 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
81 98 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
254 271 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
161 165 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
335 355 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
249 271 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
166 185 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
8 389 SUPERFAMILY SSF103473 MFS general substrate transporter
8 389 InterPro IPR036259 MFS transporter superfamily
324 334 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
49 70 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
21 349 Pfam PF07690 Major Facilitator Superfamily
21 349 InterPro IPR011701 Major facilitator superfamily
302 324 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
278 296 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
212 234 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
99 103 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.934
Likely same site as FPocket 6 2.5 Å 22 shared residues 76% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.041
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Surrounding area
Pocket 3 P2Rank #3
0.034
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Surrounding area
Pocket 4 P2Rank #4
0.024
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Surrounding area
Pocket 5 P2Rank #5
0.02
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #16
0.605
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Surrounding area
Pocket 2 FPocket #13
0.383
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Surrounding area
Pocket 3 FPocket #6
0.347 Unusual size
Likely same site as P2Rank 1 2.5 Å 22 shared residues 76% of smaller site
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Surrounding area
Pocket 4 FPocket #23
0.203 Unusual size
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GLM8
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_05610
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GLM8