Protein profile

KP13_04911

PTS system glucose-specific EIICB component

Genome: KpKP13

Gene: ptsG AHE45271.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GRI2
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Amino acids 477
Annotations 10
Features 53
PDB binders 0
Druggability 0.928

Overview

Basic information about this protein and its source genome.

Accession
KP13_04911
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
ptsG AHE45271.1
Status
annotated
Amino acids
477
Structure source
AlphaFold + ColabFold
EC
2.7.1.199
GO
GO:1904659 The process in which D-glucose is transported across a membrane. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0005355 OBSOLETE. Enables the transfer of the hexose monosaccharide glucose from one side of a membrane to the other. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
49.513
DEG E-value
1.88e-152
Localization
CytoplasmicMembrane
ColabFold pLDDT
91.33

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.928
Structure A0A0H3GRI2
Pocket Pocket 2
P2Rank 0.865
Structure A0A0H3GRI2
Pocket Pocket 1
ColabFold model
FPocket 0.372 · Pocket 23
P2Rank 0.94 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 125 / 4744 genomes with a hit
Normalized 0.026

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 9 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

9
  • GO:1904659 The process in which D-glucose is transported across a membrane.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0005355 OBSOLETE. Enables the transfer of the hexose monosaccharide glucose from one side of a membrane to the other.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0055056 Enables the transfer of the D-enantiomer of the hexose monosaccharide glucose from one side of a membrane to the other.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090564 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + glucose(out) = protein cysteine + glucose phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

53 records
Show feature table
Start End DB Term Name
154 176 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
377 477 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
298 303 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
250 272 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
72 76 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
3 388 ProSiteProfiles PS51103 PTS_EIIC type-1 domain profile.
3 388 InterPro IPR013013 Phosphotransferase system, EIIC component, type 1
40 50 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
282 304 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
397 475 FunFam G3DSA:3.30.1360.60:FF:000001 PTS system glucose-specific IIBC component PtsG
13 320 Pfam PF02378 Phosphotransferase system, EIIC
13 320 InterPro IPR003352 Phosphotransferase system, EIIC
332 353 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
354 376 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
2 476 PANTHER PTHR30009 CYTOCHROME C-TYPE SYNTHESIS PROTEIN AND PTS TRANSMEMBRANE COMPONENT
309 331 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
116 133 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
133 151 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
113 132 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 15 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
51 71 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
79 101 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
354 376 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
191 213 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
52 331 NCBIfam TIGR00852 maltose/glucose-specific PTS transporter subunit IIC
52 331 InterPro IPR004719 Phosphotransferase system, maltose/glucose-specific subfamily IIC component
414 431 ProSitePatterns PS01035 PTS EIIB domains cysteine phosphorylation site signature.
414 431 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
304 331 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
399 474 CDD cd00212 PTS_IIB_glc
399 474 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
53 72 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
396 477 Gene3D G3DSA:3.30.1360.60 Glucose permease domain IIB
396 477 InterPro IPR036878 Glucose permease domain IIB
2 475 NCBIfam TIGR02002 glucose-specific PTS transporter subunit IIBC
2 475 InterPro IPR011299 PTS system glucose-specific IIBC component
273 277 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
405 436 Pfam PF00367 phosphotransferase system, EIIB
405 436 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
152 172 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
399 477 ProSiteProfiles PS51098 PTS_EIIB type-1 domain profile.
399 477 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
77 101 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
278 297 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
16 39 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
173 253 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
102 112 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
378 460 NCBIfam TIGR00826 glucose PTS transporter subunit EIIB
378 460 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
401 474 SUPERFAMILY SSF55604 Glucose permease domain IIB
401 474 InterPro IPR036878 Glucose permease domain IIB
254 272 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GRI2
AlphaFold — — full sequence — Viewing
ColabFold KP13_04911
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.928
12 0.325

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 15.39 0.752
2 2.31 0.059
3 2.2 0.053
4 2.08 0.047
5 1.71 0.03