Protein profile

KP13_03901

Gamma-glutamyl-gamma-aminobutyrate hydrolase

Genome: KpKP13

Gene: AHE45353.1 puuD Structure source: AlphaFold + ColabFold UniProt A0A0H3GMJ3
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Amino acids 254
Annotations 6
Features 11
PDB binders 0
Druggability 0.324

Overview

Basic information about this protein and its source genome.

Accession
KP13_03901
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE45353.1 puuD
Status
annotated
Amino acids
254
Structure source
AlphaFold + ColabFold
EC
3.5.1.94
GO
GO:0016811 Catalysis of the hydrolysis of any non-peptide carbon-nitrogen bond in a linear amide. GO:0016787 Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0033969 Catalysis of the reaction: 4-(L-gamma-glutamylamino)butanoate + H2O = 4-aminobutanoate + L-glutamate. GO:0006598 The chemical reactions and pathways resulting in the breakdown of polyamines, any organic compound containing two or more amino groups.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
44.958
DEG E-value
3.97e-63
Localization
Cytoplasmic
ColabFold pLDDT
96.76

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.324
Structure A0A0H3GMJ3
Pocket Pocket 4
P2Rank 0.513
Structure A0A0H3GMJ3
Pocket Pocket 1
ColabFold model
FPocket 0.103 · Pocket 2
P2Rank 0.243 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 46 / 4744 genomes with a hit
Normalized 0.01

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0016811 Catalysis of the hydrolysis of any non-peptide carbon-nitrogen bond in a linear amide.
  • GO:0016787 Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0033969 Catalysis of the reaction: 4-(L-gamma-glutamylamino)butanoate + H2O = 4-aminobutanoate + L-glutamate.
  • GO:0006598 The chemical reactions and pathways resulting in the breakdown of polyamines, any organic compound containing two or more amino groups.

Sequence Features

Domain/signature hits from InterPro and related databases.

11 records
Show feature table
Start End DB Term Name
31 250 ProSiteProfiles PS51273 Glutamine amidotransferase type 1 domain profile.
10 241 CDD cd01745 GATase1_2
5 254 Gene3D G3DSA:3.40.50.880 -
5 254 InterPro IPR029062 Class I glutamine amidotransferase-like
5 252 FunFam G3DSA:3.40.50.880:FF:000030 Gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD
5 244 PANTHER PTHR43235 GLUTAMINE AMIDOTRANSFERASE PB2B2.05-RELATED
5 244 InterPro IPR044668 Peptidase C26, gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD-like
8 224 Pfam PF07722 Peptidase C26
8 224 InterPro IPR011697 Peptidase C26
7 247 SUPERFAMILY SSF52317 Class I glutamine amidotransferase-like
7 247 InterPro IPR029062 Class I glutamine amidotransferase-like

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GMJ3
AlphaFold — — full sequence — Viewing
ColabFold KP13_03901
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.324

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 3.22 0.111
2 2.3 0.058
3 0.92 0.004