KpKP13 Protein target profile

Inner membrane protein

Accession: KP13_03875

Gene: AHE45378.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GR44
Export view
Length 711
Pocket druggability (P2Rank · AlphaFold DB model) 0.951
Functional annotation 0 EC 1 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Moderate predicted binding pocket FPocket selected-pocket score 0.47. This supports review but is not experimental validation. Second pocket predictor agrees P2Rank predicted binding-site probability 0.95. Review the complete evidence below

Risks to review

Microbiome similarity 118 of 4744 screened genomes (2.5%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.95) — check the ligand evidence before deprioritizing on off-target risk alone.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
2.5% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
87.02 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.951
Structure A0A0H3GR44
Pocket Pocket 1
Druggability (FPocket) 0.467
Structure A0A0H3GR44
Pocket Pocket 37
ColabFold model
P2Rank 0.941 · Pocket 1
FPocket 0.882 · Pocket 33
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 118 / 4744 genomes with a hit
Prevalence 2.5%

Sequence

Primary amino-acid sequence viewer.

MISPLLRRYTWNSAWLYNVRIFIALCGTTLFPWWIGEVKLTIPLTLGVVAAALTDLDDRLAGRLRNLAITLVCFFIASASVELLFPWPPLFALGLTVSTIGFILLGGLGQRYATIAFGALLIAIYTMLGVTLYDHWYLQPLFLLAGAVWYNLLTLSGHLIFPIRPLQDNLARSYEQLARYLELKSRLFDPDLEDESQAPLYDLALANGQLVATLNQTKVSLLTRLRGDRGQRGTRRTLQYYFVAQDIHERASSSHIQYQTLRDQFRYSDVMFRFQRMLSMQAQACQKLSRAILLREPYQHDAHFERAFMHLDAALERVRAGGASDEQLNALGYLLNNLRAIDAQLATIESVQTTAPAGSNTETLLADDRLGGLSDIWLRLQRNMSPESALFRHAVRMSLVLCAGYAFIQFTGLQHGYWILLTSLFVCQPNYNATRHRLALRIIGTLVGVAIGLPVLLLVPSVEGQLLLIVLTGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEGFEVALPRIIDTLIGCAIAWAAVSFIWPDWKFRNLPRVLDRAMNANCRYLDAILEQYHQGRDNRLAYRVARRDAYNRDAELASVVSNLSTEPRADATQRETAFRLLCLNHTFTSYISALGAHREKLSTPEILALLDDAVCYVDDALHHTPADEQRVQQALNSLQSRIHHLEPRADSKEPLVLQQIGLLLALLPEICRLQQRVHAQTE

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

1
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

42 records
Show feature table
Start End DB Term Name
15 34 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
115 136 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
109 114 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
480 483 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 14 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
142 163 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
141 163 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
509 531 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
484 503 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
438 458 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
91 108 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
164 388 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
532 711 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
504 508 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
40 56 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
8 705 NCBIfam TIGR01666 YccS family putative transporter
8 705 InterPro IPR010019 Integral membrane protein, YccS
432 437 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
437 459 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
112 131 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
517 534 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
466 483 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
68 85 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
389 408 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
488 510 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
414 431 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
66 345 Pfam PF12805 FUSC-like inner membrane protein yccS
66 345 InterPro IPR032692 Integral membrane protein YccS, N-terminal
137 141 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
400 422 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
57 67 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
8 705 NCBIfam TIGR01667 YccS/YhfK family putative transporter
8 705 InterPro IPR010020 Integral membrane protein, YccS/YhfK
459 463 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
12 671 PANTHER PTHR30509 P-HYDROXYBENZOIC ACID EFFLUX PUMP SUBUNIT-RELATED
86 90 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
83 105 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
406 525 Pfam PF13515 Fusaric acid resistance protein-like
464 479 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
409 413 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
35 39 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

Download VMD script Full viewer

Loading 3D structure...

Drag to rotate — click the view, then scroll to zoom.

Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.951
Show in viewer
Surrounding area
Pocket 2 P2Rank #2
0.476
Likely same site as FPocket 37 0.4 Å 23 shared residues 100% of smaller site
Show in viewer
Surrounding area
Pocket 3 P2Rank #3
0.439
Show in viewer
Surrounding area
Pocket 4 P2Rank #4
0.287
Likely same site as FPocket 35 1.7 Å 4 shared residues 80% of smaller site
Show in viewer
Surrounding area
Pocket 5 P2Rank #5
0.274
Show in viewer
Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #37
0.467 Unusual size
Likely same site as P2Rank 2 0.4 Å 23 shared residues 100% of smaller site
Show in viewer
Surrounding area
Pocket 2 FPocket #28
0.455
Show in viewer
Surrounding area
Pocket 3 FPocket #35
0.307
Likely same site as P2Rank 4 1.7 Å 4 shared residues 80% of smaller site
Show in viewer
Surrounding area
Pocket 4 FPocket #54
0.2 Unusual size
Show in viewer
Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GR44
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_03875
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GR44