Protein profile

KP13_04170

Nicotinate phosphoribosyltransferase

Genome: KpKP13

Gene: AHE45401.1 pncB Structure source: AlphaFold + ColabFold UniProt A0A0H3GV33
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Amino acids 412
Annotations 6
Features 19
PDB binders 0
Druggability 0.987

Overview

Basic information about this protein and its source genome.

Accession
KP13_04170
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE45401.1 pncB
Status
annotated
Amino acids
412
Structure source
AlphaFold + ColabFold
EC
6.3.4.21
GO
GO:0004516 Catalysis of the reaction: nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate. GO:0009435 The chemical reactions and pathways resulting in the formation of nicotinamide adenine dinucleotide (NAD+), a coenzyme that interconverts with its reduced form, NADH, in many redox and catabolic reactions. NAD+ is derived from various sources including vitamin B3. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0016757 Catalysis of the transfer of a glycosyl group from one compound (donor) to another (acceptor). GO:0034355 The chemical reactions and pathways resulting in the formation of nicotinamide-adenine dinucleotide (NAD+) from vitamin B3 derivatives (including nicotinic acid (NA) and nicotinamide (NAM)), beta-nicotinamide D-ribonucleotide (NMN), nicotinamide riboside (NR) or nicotinate riboside (NAR), without de novo synthesis.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
94.32

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.987
Structure A0A0H3GV33
Pocket Pocket 1
P2Rank 0.966
Structure A0A0H3GV33
Pocket Pocket 1
ColabFold model
FPocket 0.879 · Pocket 1
P2Rank 0.963 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 123 / 4744 genomes with a hit
Normalized 0.026

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0004516 Catalysis of the reaction: nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate.
  • GO:0009435 The chemical reactions and pathways resulting in the formation of nicotinamide adenine dinucleotide (NAD+), a coenzyme that interconverts with its reduced form, NADH, in many redox and catabolic reactions. NAD+ is derived from various sources including vitamin B3.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0016757 Catalysis of the transfer of a glycosyl group from one compound (donor) to another (acceptor).
  • GO:0034355 The chemical reactions and pathways resulting in the formation of nicotinamide-adenine dinucleotide (NAD+) from vitamin B3 derivatives (including nicotinic acid (NA) and nicotinamide (NAM)), beta-nicotinamide D-ribonucleotide (NMN), nicotinamide riboside (NR) or nicotinate riboside (NAR), without de novo synthesis.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
181 406 Pfam PF04095 Nicotinate phosphoribosyltransferase (NAPRTase) family
181 406 InterPro IPR041525 Nicotinate/nicotinamide phosphoribosyltransferase
17 407 Hamap MF_00570 Nicotinate phosphoribosyltransferase [pncB].
17 407 InterPro IPR006406 Nicotinate phosphoribosyltransferase
16 400 PANTHER PTHR11098 NICOTINATE PHOSPHORIBOSYLTRANSFERASE
16 400 InterPro IPR007229 Nicotinate phosphoribosyltransferase family
13 412 FunFam G3DSA:3.20.140.10:FF:000001 Nicotinate phosphoribosyltransferase
13 150 SUPERFAMILY SSF54675 Nicotinate/Quinolinate PRTase N-terminal domain-like
132 404 SUPERFAMILY SSF51690 Nicotinate/Quinolinate PRTase C-terminal domain-like
132 404 InterPro IPR036068 Nicotinate phosphoribosyltransferase-like, C-terminal
22 388 CDD cd01401 PncB_like
22 388 InterPro IPR006406 Nicotinate phosphoribosyltransferase
20 406 NCBIfam TIGR01514 nicotinate phosphoribosyltransferase
20 406 InterPro IPR006406 Nicotinate phosphoribosyltransferase
13 412 Gene3D G3DSA:3.20.140.10 nicotinate phosphoribosyltransferase
11 406 PIRSF PIRSF000484 NAPRT
11 406 InterPro IPR007229 Nicotinate phosphoribosyltransferase family
25 142 Pfam PF17767 Nicotinate phosphoribosyltransferase (NAPRTase) N-terminal domain
25 142 InterPro IPR040727 Nicotinate phosphoribosyltransferase, N-terminal domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GV33
AlphaFold — — full sequence — Viewing
ColabFold KP13_04170
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.987

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 35.78 0.954