Protein profile

KP13_04194

Tetraacyldisaccharide 4'-kinase

Genome: KpKP13

Gene: AHE45425.1 lpxK Structure source: AlphaFold + ColabFold UniProt A0A0H3GL20
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Amino acids 326
Annotations 6
Features 14
PDB binders 2
Druggability 0.786

Overview

Basic information about this protein and its source genome.

Accession
KP13_04194
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE45425.1 lpxK
Status
annotated
Amino acids
326
Structure source
AlphaFold + ColabFold
EC
2.7.1.130
GO
GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0009029 Catalysis of the reaction: a lipid A disaccharide + ATP = a lipid IVA + ADP + H+. GO:0009245 The chemical reactions and pathways resulting in the formation of lipid A, the glycolipid group of bacterial lipopolysaccharides, consisting of four to six fatty acyl chains linked to two glucosamine residues. Further modifications of the backbone are common. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0009244 The chemical reactions and pathways resulting in the formation of the core region of bacterial lipopolysaccharides, which contains ten saccharide residues.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
77.538
DEG E-value
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
93.86

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.786
Structure A0A0H3GL20
Pocket Pocket 1
P2Rank 0.945
Structure A0A0H3GL20
Pocket Pocket 1
ColabFold model
FPocket 0.762 · Pocket 8
P2Rank 0.954 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 139 / 4744 genomes with a hit
Normalized 0.029

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0009029 Catalysis of the reaction: a lipid A disaccharide + ATP = a lipid IVA + ADP + H+.
  • GO:0009245 The chemical reactions and pathways resulting in the formation of lipid A, the glycolipid group of bacterial lipopolysaccharides, consisting of four to six fatty acyl chains linked to two glucosamine residues. Further modifications of the backbone are common.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0009244 The chemical reactions and pathways resulting in the formation of the core region of bacterial lipopolysaccharides, which contains ten saccharide residues.

Sequence Features

Domain/signature hits from InterPro and related databases.

14 records
Show feature table
Start End DB Term Name
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 325 Hamap MF_00409 Tetraacyldisaccharide 4'-kinase [lpxK].
1 325 InterPro IPR003758 Tetraacyldisaccharide 4'-kinase
35 326 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
12 34 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
10 319 PANTHER PTHR42724 TETRAACYLDISACCHARIDE 4'-KINASE
10 319 InterPro IPR003758 Tetraacyldisaccharide 4'-kinase
46 162 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
46 162 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
12 34 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
13 318 Pfam PF02606 Tetraacyldisaccharide-1-P 4'-kinase
13 318 InterPro IPR003758 Tetraacyldisaccharide 4'-kinase
19 312 NCBIfam TIGR00682 tetraacyldisaccharide 4'-kinase
19 312 InterPro IPR003758 Tetraacyldisaccharide 4'-kinase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GL20
AlphaFold — — full sequence — Viewing
ColabFold KP13_04194
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.786
23 0.307

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 22.07 0.877

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

2 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
LP4
4LKV
O67572 711.9 Da LogP 4.91 TPSA 212.3 2 viol. ✓ Clean CCCCCCCCCCC[C@H](CC(=O)N[C@@H]1[C@H]([C@@H]([C@…
LP5
4LKV
O67572 711.9 Da LogP 4.91 TPSA 212.3 2 viol. ✓ Clean CCCCCCCCCCC[C@H](CC(=O)N[C@@H]1[C@H]([C@@H]([C@…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.