Protein profile

KP13_04226

Macrolide-specific efflux protein macA

Genome: KpKP13

Gene: macA AHE45457.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GQL5
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Amino acids 371
Annotations 9
Features 19
PDB binders 0
Druggability 0.144

Overview

Basic information about this protein and its source genome.

Accession
KP13_04226
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
macA AHE45457.1
Status
annotated
Amino acids
371
Structure source
AlphaFold + ColabFold
GO
GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0019898 The component of a membrane consisting of gene products and protein complexes that are loosely bound to one of its surfaces, but not integrated into the hydrophobic region. GO:1990961 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:1990195 A bacterial transmembrane transporter complex that spans the entire cell membrane system and possesses ATP-dependent xenobiotic transport activity pumping drugs (typically antibiotics) and other toxins directly from the cytosol out of the bacterial cell. Typically, it is trimeric consisting of a inner membrane ATPase (IMP), a periplasmic membrane fusion protein (MFP) and an outer membrane factor (OMF). In E. coli, macrolide transporter complexes may consists of MacB (IMP), MacA (MFP) and TolC (OMF) or AcrB (IMP), AcrA (MFP) and TolC (OMF). Trimeric TolC is a common OMF found in many macrolide transporter complexes. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
90.95

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.144
Structure A0A0H3GQL5
Pocket Pocket 5
P2Rank
Structure A0A0H3GQL5
Pocket No pockets
ColabFold model
FPocket 0.313 · Pocket 12
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 92 / 4744 genomes with a hit
Normalized 0.019

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Gene Ontology (GO)

9
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0019898 The component of a membrane consisting of gene products and protein complexes that are loosely bound to one of its surfaces, but not integrated into the hydrophobic region.
  • GO:1990961 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:1990195 A bacterial transmembrane transporter complex that spans the entire cell membrane system and possesses ATP-dependent xenobiotic transport activity pumping drugs (typically antibiotics) and other toxins directly from the cytosol out of the bacterial cell. Typically, it is trimeric consisting of a inner membrane ATPase (IMP), a periplasmic membrane fusion protein (MFP) and an outer membrane factor (OMF). In E. coli, macrolide transporter complexes may consists of MacB (IMP), MacA (MFP) and TolC (OMF) or AcrB (IMP), AcrA (MFP) and TolC (OMF). Trimeric TolC is a common OMF found in many macrolide transporter complexes.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0030313 An envelope that surrounds a bacterial cell and includes the cytoplasmic membrane and everything external, encompassing the periplasmic space, cell wall, and outer membrane if present.
  • GO:1990281 A protein complex that is capable of efflux transmembrane transporter activity.
  • GO:0015562 Enables the transfer of a specific substance or related group of substances from the inside of the cell to the outside of the cell across a membrane.

Sequence Features

Domain/signature hits from InterPro and related databases.

19 records
Show feature table
Start End DB Term Name
9 26 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
26 371 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 25 Phobius SIGNAL_PEPTIDE Signal peptide region
218 297 Gene3D G3DSA:2.40.30.170 -
47 294 Pfam PF16576 Barrel-sandwich domain of CusB or HlyD membrane-fusion
47 294 InterPro IPR032317 RND efflux pump, membrane fusion protein, barrel-sandwich domain
10 21 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
61 217 Gene3D G3DSA:2.40.50.100 -
52 299 SUPERFAMILY SSF111369 HlyD-like secretion proteins
218 297 FunFam G3DSA:2.40.30.170:FF:000007 Macrolide transporter subunit MacA
95 182 Gene3D G3DSA:6.10.140.1990 -
95 182 InterPro IPR030190 Macrolide export protein MacA, alpha-hairpin domain superfamily
39 362 NCBIfam TIGR01730 efflux RND transporter periplasmic adaptor subunit
39 362 InterPro IPR006143 RND efflux pump, membrane fusion protein
298 365 Gene3D G3DSA:2.40.420.20 -
94 128 Coils Coil Coil
22 25 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
5 364 PANTHER PTHR30469 MULTIDRUG RESISTANCE PROTEIN MDTA
1 9 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GQL5
AlphaFold — — full sequence — Viewing
ColabFold KP13_04226
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer