Protein profile

KP13_04253

Putrescine transport ATP-binding protein potG

Genome: KpKP13

Gene: AHE45485.1 potG Structure source: AlphaFold + ColabFold UniProt A0A0H3GQU4
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Amino acids 393
Annotations 9
Features 22
PDB binders 6
Druggability 0.293

Overview

Basic information about this protein and its source genome.

Accession
KP13_04253
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE45485.1 potG
Status
annotated
Amino acids
393
Structure source
AlphaFold + ColabFold
EC
7.6.2.11
GO
GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0015417 Catalysis of the reaction: ATP + H2O + polyamine(out) = ADP + phosphate + polyamine(in). GO:0015846 The directed movement of polyamines, organic compounds containing two or more amino groups, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0043190 A complex for the transport of metabolites into and out of the cell, typically comprised of four domains; two membrane-associated domains and two ATP-binding domains at the intracellular face of the membrane, that form a central pore through the plasma membrane. Each of the four core domains may be encoded as a separate polypeptide or the domains can be fused in any one of a number of ways into multidomain polypeptides. In Bacteria and Archaebacteria, ABC transporters also include substrate binding proteins to bind substrate external to the cytoplasm and deliver it to the transporter.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
34.742
Human E-value
4.899999999999999e-24
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
41.644
DEG E-value
1.86e-87
Localization
CytoplasmicMembrane
ColabFold pLDDT
88.56

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.293
Structure A0A0H3GQU4
Pocket Pocket 3
P2Rank 0.234
Structure A0A0H3GQU4
Pocket Pocket 1
ColabFold model
FPocket 0.484 · Pocket 22
P2Rank 0.295 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 144 / 4744 genomes with a hit
Normalized 0.03

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0015417 Catalysis of the reaction: ATP + H2O + polyamine(out) = ADP + phosphate + polyamine(in).
  • GO:0015846 The directed movement of polyamines, organic compounds containing two or more amino groups, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0043190 A complex for the transport of metabolites into and out of the cell, typically comprised of four domains; two membrane-associated domains and two ATP-binding domains at the intracellular face of the membrane, that form a central pore through the plasma membrane. Each of the four core domains may be encoded as a separate polypeptide or the domains can be fused in any one of a number of ways into multidomain polypeptides. In Bacteria and Archaebacteria, ABC transporters also include substrate binding proteins to bind substrate external to the cytoplasm and deliver it to the transporter.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0015847 The directed movement of putrescine into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Putrescine is 1,4-diaminobutane, the polyamine formed by decarboxylation of ornithine and the metabolic precursor of spermidine and spermine.

Sequence Features

Domain/signature hits from InterPro and related databases.

22 records
Show feature table
Start End DB Term Name
52 193 Pfam PF00005 ABC transporter
52 193 InterPro IPR003439 ABC transporter-like, ATP-binding domain
254 336 Gene3D G3DSA:2.40.50.100 -
36 266 ProSiteProfiles PS50893 ATP-binding cassette, ABC transporter-type domain profile.
36 266 InterPro IPR003439 ABC transporter-like, ATP-binding domain
60 243 SMART SM00382 AAA_5
60 243 InterPro IPR003593 AAA+ ATPase domain
35 273 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
35 273 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
33 253 FunFam G3DSA:3.40.50.300:FF:000133 Spermidine/putrescine import ATP-binding protein PotA
35 253 Gene3D G3DSA:3.40.50.300 -
35 253 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
166 180 ProSitePatterns PS00211 ABC transporters family signature.
166 180 InterPro IPR017871 ABC transporter-like, conserved site
311 391 Pfam PF08402 TOBE domain
311 391 InterPro IPR013611 Transport-associated OB, type 2
250 340 FunFam G3DSA:2.40.50.100:FF:000034 Spermidine/putrescine import ATP-binding protein PotA
268 391 SUPERFAMILY SSF50331 MOP-like
268 391 InterPro IPR008995 Molybdate/tungstate binding, C-terminal
34 391 PANTHER PTHR42781 SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA
66 391 NCBIfam TIGR01187 polyamine ABC transporter ATP-binding protein
66 391 InterPro IPR005893 Spermidine/putrescine import ATP-binding protein PotA-like

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GQU4
AlphaFold — — full sequence — Viewing
ColabFold KP13_04253
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.293
5 0.291

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 2.58 0.074
2 2.52 0.07
3 2.07 0.046
4 1.34 0.015
5 0.61 0.0

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

56 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
2BA
7AHE
Q9KIF7 658.4 Da LogP -1.63 TPSA 309.7 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@H]4[C@H](O3)C…
AGS
6CVL
P30750 523.2 Da LogP -1.51 TPSA 262.1 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
ANP
1OXV
Q97UY8 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
BET
7AHH
Q9KIF7 118.2 Da LogP -0.22 TPSA 37.3 ✓ Ro5 ✓ Clean C[N+](C)(C)CC(=O)O
NH4
1G29
Q9YGA6 18.0 Da LogP 0.38 TPSA 36.5 ✓ Ro5 ✓ Clean [NH4+]
POP
2D62
O57933 176.0 Da LogP -2.08 TPSA 129.9 ✓ Ro5 ✓ Clean O[P@@](=O)([O-])O[P@@](=O)(O)[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.