KpKP13 Protein target profile

Aldose 1-epimerase

Accession: KP13_02992

Gene: AHE45625.1 galM 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A0H3GK63
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Length 347
Pocket druggability (P2Rank · AlphaFold DB model) 0.791
Functional annotation 1 EC 10 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Core gene across strains Marked as conserved by both pan-genome tools: Roary and CoreCruncher. Second pocket predictor agrees P2Rank predicted binding-site probability 0.79. Structure available AlphaFold DB model + ColabFold model

Risks to review

Human similarity Top human hit identity: 39.037%. This target also has a predicted binding pocket (P2Rank 0.79) — check the ligand evidence before deprioritizing on off-target risk alone. Microbiome similarity 98 of 4744 screened genomes (2.1%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.79) — check the ligand evidence before deprioritizing on off-target risk alone. Weak predicted binding pocket FPocket selected-pocket score 0.30.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
Hit
Human identity (%)
39.037 Lower values reduce human off-target concern.
Human E-value
1.1999999999999999e-35
Gut microbiome similarity
2.1% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
Y
DEG identity (%)
40.356 Higher values support similarity to known essential genes.
DEG E-value
2.2e-81 Smaller values mean stronger essential-gene similarity.

Structure confidence

ColabFold pLDDT
97.5 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.791
Structure A0A0H3GK63
Pocket Pocket 1
Druggability (FPocket) 0.3
Structure A0A0H3GK63
Pocket Pocket 2
ColabFold model
P2Rank 0.693 · Pocket 1
FPocket 0.225 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 98 / 4744 genomes with a hit
Prevalence 2.1%

Sequence

Primary amino-acid sequence viewer.

MLTQTTALAPDGQPWQQVTLRNKSGMTVTVADWGATLLSAEVPLADGSLRRPLLGCAKLEDYARQAAFLGASVGRYANRIGHSRFPLDGQVVNVTPSNDAGHQLHGGPEGFDKRRWRIVRADEQEVLFALTSPDGDQGFPGTLQATAHYRLSDDNRIAITYRATVDQPCPVNMTNHVYFNLDGEQGDVRQHQLQILAQRYLPVESDGIPGGELKDVANTSFDFRQPKTIAADFLADADQQKVKGYDHAFLLDAKGDASQPAAQVWSQDGKLQMTVYTSAPALQFYSGNYLGGTPSQTTEPYADWQGLALESEFLPDSPNHPQWPQPDCVLRPGQEYVSLTEYQFIAR

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 10 GO

Subcellular localization

Localization
Periplasmic

Enzyme Commission (EC)

1

Gene Ontology (GO)

10
  • GO:0030246 Binding to a carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates.
  • GO:0006012 The chemical reactions and pathways involving galactose, the aldohexose galacto-hexose. D-galactose is widely distributed in combined form in plants, animals and microorganisms as a constituent of oligo- and polysaccharides; it also occurs in galactolipids and as its glucoside in lactose and melibiose.
  • GO:0016853 Catalysis of the geometric or structural changes within one molecule. Isomerase is the systematic name for any enzyme of EC class 5.
  • GO:0005975 The chemical reactions and pathways involving carbohydrates, any of a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0019318 The chemical reactions and pathways involving a hexose, any monosaccharide with a chain of six carbon atoms in the molecule.
  • GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
  • GO:0004034 Catalysis of the reaction: alpha-D-glucose = beta-D-glucose. Also acts on L-arabinose, D-xylose, D-galactose, maltose and lactose.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0033499 The chemical reactions and pathways resulting in the breakdown of galactose, via the intermediate UDP-galactose.
  • GO:0006006 The chemical reactions and pathways involving glucose, the aldohexose gluco-hexose. D-glucose is dextrorotatory and is sometimes known as dextrose; it is an important source of energy for living organisms and is found free as well as combined in homo- and hetero-oligosaccharides and polysaccharides.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
172 181 ProSitePatterns PS00545 Aldose 1-epimerase putative active site.
172 181 InterPro IPR018052 Aldose 1-epimerase, conserved site
17 342 Pfam PF01263 Aldose 1-epimerase
17 342 InterPro IPR008183 Aldose 1-/Glucose-6-phosphate 1-epimerase
1 347 PIRSF PIRSF005096 GALM
1 347 InterPro IPR015443 Aldose 1-epimerase
3 344 SUPERFAMILY SSF74650 Galactose mutarotase-like
3 344 InterPro IPR011013 Galactose mutarotase-like domain superfamily
17 344 CDD cd09019 galactose_mutarotase_like
17 344 InterPro IPR047215 Galactose mutarotase-like
12 344 NCBIfam TIGR02636 galactose-1-epimerase
12 344 InterPro IPR013458 Aldose 1-epimerase, bacterial
9 345 FunFam G3DSA:2.70.98.10:FF:000002 Aldose 1-epimerase
8 345 Gene3D G3DSA:2.70.98.10 -
8 345 InterPro IPR014718 Glycoside hydrolase-type carbohydrate-binding
18 344 PANTHER PTHR10091 ALDOSE-1-EPIMERASE

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.791
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Surrounding area
Pocket 2 P2Rank #2
0.069
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #2
0.3
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Surrounding area
Residue sets
UniProt: Active site:176-176 Proton donor
UniProt: Active site:310-310 Proton acceptor
UniProt: Binding site:176-178
UniProt: Binding site:246-246
UniProt: Binding site:78-79
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A0H3GK63
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_02992
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A0H3GK63