KpKP13 Protein target profile

putative mechanosensitive ion channel

Accession: KP13_03425

Gene: AHE45802.1 3D evidence: AlphaFold DB model + ColabFold model UniProt A0A6A8EK81
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Length 418
Pocket druggability (P2Rank · AlphaFold DB model) 0.073
Functional annotation 0 EC 5 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Strong predicted binding pocket FPocket selected-pocket score 0.91. This is a computational pocket signal, not binding validation. Structure available AlphaFold DB model + ColabFold model

Risks to review

Microbiome similarity 18 of 4744 screened genomes (0.4%) have a significant similarity hit. This target also has a predicted binding pocket (FPocket 0.91) — check the ligand evidence before deprioritizing on off-target risk alone. Mixed conservation Core/accessory calls are not fully concordant. Weak second pocket-predictor signal P2Rank predicted binding-site probability 0.07.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
0.4% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
0.0 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
87.42 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.073
Structure A0A6A8EK81
Pocket Pocket 1
Druggability (FPocket) 0.91
Structure A0A6A8EK81
Pocket Pocket 4
ColabFold model
P2Rank 0.099 · Pocket 1
FPocket 0.501 · Pocket 2
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 18 / 4744 genomes with a hit
Prevalence 0.4%

Sequence

Primary amino-acid sequence viewer.

MAYVNHLLTFIESNPALAVGFNLILLLLSGIFAHLLCKFLLIKVVRKVFFSSHKQDVPLEKDRRIAEKLSNFIPVIIVYYVLQFMPAMPASLVTAINTICGILFFIFMSVFFNEMLDIVNSSYLRKTKRKNHSIKGYIQIGKILVHILAAIMILAVMSNKSPIIIISSLGAVAAVLMIVFQHTLLSLVANVQLSSNDVLQLGDWIEMPDKNLSGEVTDIALHTITICNWDNTISRIPTKNFLTETYTNWQAMFSSGARRIMRSIAIDQHSVRFLDQEMLSSMLTIRGVSEPLATLLDGRDPGGVADRWFIDNGLTNLTLFRHYLMHYLAERPDIIKEMYIVARTLKPSPSGIPLEIYCFTTSTLWKDYENTQSAIFEYITAVAGQFSLRLYQYPAGHDFWRLSQEHAARTGLPPSAEG

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

5
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0008381 Enables the transmembrane transfer of an monoatomic ion by a channel that opens in response to a mechanical stress.
  • GO:0071470 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating an increase or decrease in the concentration of solutes outside the organism or cell.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

24 records
Show feature table
Start End DB Term Name
69 88 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
137 157 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
140 284 Pfam PF00924 Mechanosensitive ion channel
140 284 InterPro IPR006685 Mechanosensitive ion channel MscS
42 68 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
93 115 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 19 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
163 180 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
183 249 SUPERFAMILY SSF50182 Sm-like ribonucleoproteins
183 249 InterPro IPR010920 LSM domain superfamily
181 418 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
89 93 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
136 158 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
158 162 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
69 88 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
15 37 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
11 405 PANTHER PTHR30414 UNCHARACTERIZED
11 405 InterPro IPR030192 Miniconductance mechanosensitive channel YbdG
94 116 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
163 185 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
117 136 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
20 41 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
199 249 Gene3D G3DSA:2.30.30.60 -
199 249 InterPro IPR023408 Mechanosensitive ion channel MscS, beta-domain superfamily

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.073
Likely same site as FPocket 7 1.8 Å 5 shared residues 100% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.055
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Surrounding area
Pocket 3 P2Rank #3
0.053
Likely same site as FPocket 7 5.7 Å 5 shared residues 83% of smaller site
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Surrounding area
Pocket 4 P2Rank #4
0.051
Likely same site as FPocket 4 2.5 Å 11 shared residues 100% of smaller site
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Surrounding area
Pocket 5 P2Rank #5
0.043
Likely same site as FPocket 2 2.0 Å 7 shared residues 88% of smaller site
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #4
0.91 Unusual size
Likely same site as P2Rank 4 2.5 Å 11 shared residues 100% of smaller site
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Surrounding area
Pocket 2 FPocket #2
0.597
Likely same site as P2Rank 5 2.0 Å 7 shared residues 88% of smaller site
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Surrounding area
Pocket 3 FPocket #8
0.229
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Surrounding area
Pocket 4 FPocket #7
0.2
Likely same site as P2Rank 1 1.8 Å 5 shared residues 100% of smaller site
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A6A8EK81
AlphaFold DB — — full sequence — Viewing
ColabFold KP13_03425
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A6A8EK81