Protein profile

KP13_03453

Carboxylate-amine ligase

Genome: KpKP13

Gene: AHE45829.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GPE1
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Amino acids 371
Annotations 4
Features 10
PDB binders 0
Druggability 0.225

Overview

Basic information about this protein and its source genome.

Accession
KP13_03453
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE45829.1
Status
annotated
Amino acids
371
Structure source
AlphaFold + ColabFold
GO
GO:0016879 Catalysis of the joining of two molecules, or two groups within a single molecule, via a carbon-nitrogen bond, with the concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate. GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. GO:0042398 The chemical reactions and pathways resulting in the formation of compounds derived from amino acids, organic acids containing one or more amino substituents. GO:0004357 Catalysis of the reaction: L-cysteine + L-glutamate + ATP = L-gamma-glutamyl-L-cysteine + ADP + 2 H+ + phosphate.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Cytoplasmic
ColabFold pLDDT
94.88

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.225
Structure A0A0H3GPE1
Pocket Pocket 26
P2Rank 0.97
Structure A0A0H3GPE1
Pocket Pocket 1
ColabFold model
FPocket 0.618 · Pocket 15
P2Rank 0.952 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 56 / 4744 genomes with a hit
Normalized 0.012

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0016879 Catalysis of the joining of two molecules, or two groups within a single molecule, via a carbon-nitrogen bond, with the concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate.
  • GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
  • GO:0042398 The chemical reactions and pathways resulting in the formation of compounds derived from amino acids, organic acids containing one or more amino substituents.
  • GO:0004357 Catalysis of the reaction: L-cysteine + L-glutamate + ATP = L-gamma-glutamyl-L-cysteine + ADP + 2 H+ + phosphate.

Sequence Features

Domain/signature hits from InterPro and related databases.

10 records
Show feature table
Start End DB Term Name
1 371 Gene3D G3DSA:3.30.590.20 -
4 369 SUPERFAMILY SSF55931 Glutamine synthetase/guanido kinase
4 369 InterPro IPR014746 Glutamine synthetase/guanido kinase, catalytic domain
4 370 PANTHER PTHR36510 GLUTAMATE--CYSTEINE LIGASE 2-RELATED
13 296 NCBIfam TIGR02050 YbdK family carboxylate-amine ligase
13 296 InterPro IPR011793 Putative glutamate--cysteine ligase YbdK
1 371 Hamap MF_01609 Glutamate--cysteine ligase [gshA].
1 371 InterPro IPR011793 Putative glutamate--cysteine ligase YbdK
14 292 Pfam PF04107 Glutamate-cysteine ligase family 2(GCS2)
14 292 InterPro IPR006336 Glutamate--cysteine ligase, GCS2

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GPE1
AlphaFold — — full sequence — Viewing
ColabFold KP13_03453
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
26 0.225

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 38.12 0.959
2 6.34 0.319
3 1.64 0.027