Protein profile

KP13_31981

PTS system sucrose-specific EIIBC component

Genome: KpKP13

Gene: AHE45894.1 scrA Structure source: AlphaFold + ColabFold UniProt A0A0H3GKV7
Export view
Amino acids 456
Annotations 7
Features 55
PDB binders 0
Druggability 0.609

Overview

Basic information about this protein and its source genome.

Accession
KP13_31981
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
AHE45894.1 scrA
Status
annotated
Amino acids
456
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0090589 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + trehalose (out) = protein cysteine + trehalose-6-phosphate (in).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
90.7

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.609
Structure A0A0H3GKV7
Pocket Pocket 1
P2Rank 0.94
Structure A0A0H3GKV7
Pocket Pocket 1
ColabFold model
FPocket 0.97 · Pocket 3
P2Rank 0.949 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 82 / 4744 genomes with a hit
Normalized 0.017

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090589 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + trehalose (out) = protein cysteine + trehalose-6-phosphate (in).
  • GO:0015771 The directed movement of trehalose into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Trehalose is a disaccharide that consists of two molecules of glucose and is isomeric with sucrose.

Sequence Features

Domain/signature hits from InterPro and related databases.

55 records
Show feature table
Start End DB Term Name
245 267 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
351 361 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
202 212 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
327 349 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
4 82 CDD cd00212 PTS_IIB_glc
4 82 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
178 201 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
359 381 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 451 PANTHER PTHR30175 PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN
107 456 ProSiteProfiles PS51103 PTS_EIIC type-1 domain profile.
107 456 InterPro IPR013013 Phosphotransferase system, EIIC component, type 1
309 328 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
388 410 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 107 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
383 388 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
108 133 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
10 41 Pfam PF00367 phosphotransferase system, EIIB
10 41 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
19 36 ProSitePatterns PS01035 PTS EIIB domains cysteine phosphorylation site signature.
19 36 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
167 177 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
2 81 FunFam G3DSA:3.30.1360.60:FF:000001 PTS system glucose-specific IIBC component PtsG
284 306 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
153 437 NCBIfam TIGR00852 maltose/glucose-specific PTS transporter subunit IIC
153 437 InterPro IPR004719 Phosphotransferase system, maltose/glucose-specific subfamily IIC component
450 456 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
247 269 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
7 80 SUPERFAMILY SSF55604 Glucose permease domain IIB
7 80 InterPro IPR036878 Glucose permease domain IIB
234 244 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
108 130 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
389 408 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
134 144 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
174 196 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
362 382 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
268 286 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
206 228 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
409 427 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
329 350 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
145 167 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 77 Gene3D G3DSA:3.30.1360.60 Glucose permease domain IIB
1 77 InterPro IPR036878 Glucose permease domain IIB
428 449 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
4 87 ProSiteProfiles PS51098 PTS_EIIB type-1 domain profile.
4 87 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
145 166 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
425 447 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
28 111 NCBIfam TIGR00826 glucose PTS transporter subunit EIIB
28 111 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
287 308 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
110 395 Pfam PF02378 Phosphotransferase system, EIIC
110 395 InterPro IPR003352 Phosphotransferase system, EIIC
213 233 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
3 448 NCBIfam TIGR01996 sucrose-specific PTS transporter subunit IIBC
3 448 InterPro IPR010973 Phosphotransferase system, sucrose-specific IIBC component

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GKV7
AlphaFold — — full sequence — Viewing
ColabFold KP13_31981
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
29 0.113
19 0.088
2 0.063
11 0.001

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 37.62 0.94
2 5.91 0.24
3 4.45 0.155
4 2.03 0.037
5 1.91 0.032