Protein profile

KP13_03652

Nickel/cobalt efflux system rcnA

Genome: KpKP13

Gene: rcnA AHE45993.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GKJ6
Export view
Amino acids 305
Annotations 7
Features 24
PDB binders 0
Druggability 0.912

Overview

Basic information about this protein and its source genome.

Accession
KP13_03652
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
rcnA AHE45993.1
Status
annotated
Amino acids
305
Structure source
AlphaFold + ColabFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0035444 The directed movement of nickel (Ni) cations across a membrane by means of some agent such as a transporter or pore. GO:0015099 Enables the transfer of nickel (Ni) cations from one side of a membrane to the other. GO:0046583 Enables the transfer of a cation or cations from the inside of the cell to the outside of the cell across a membrane. GO:0006824 The directed movement of cobalt (Co2+) ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0032025 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cobalt ion (Co2+) stimulus.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
CytoplasmicMembrane
ColabFold pLDDT
68.66

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.912
Structure A0A0H3GKJ6
Pocket Pocket 24
P2Rank 0.68
Structure A0A0H3GKJ6
Pocket Pocket 1
ColabFold model
FPocket 0.773 · Pocket 2
P2Rank 0.553 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 45 / 4744 genomes with a hit
Normalized 0.009

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0035444 The directed movement of nickel (Ni) cations across a membrane by means of some agent such as a transporter or pore.
  • GO:0015099 Enables the transfer of nickel (Ni) cations from one side of a membrane to the other.
  • GO:0046583 Enables the transfer of a cation or cations from the inside of the cell to the outside of the cell across a membrane.
  • GO:0006824 The directed movement of cobalt (Co2+) ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0032025 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cobalt ion (Co2+) stimulus.
  • GO:0010045 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nickel cation stimulus.

Sequence Features

Domain/signature hits from InterPro and related databases.

24 records
Show feature table
Start End DB Term Name
241 263 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
78 88 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
118 179 MobiDBLite mobidb-lite consensus disorder prediction
53 75 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
208 229 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
282 304 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
52 77 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
284 302 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
89 107 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
32 51 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
10 32 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
118 171 MobiDBLite mobidb-lite consensus disorder prediction
230 240 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
90 107 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
208 230 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
108 207 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
240 262 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
13 302 Pfam PF03824 High-affinity nickel-transport protein
13 302 InterPro IPR011541 Nickel/cobalt transporter, high-affinity
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
303 305 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
264 283 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
7 153 PANTHER PTHR40659 NICKEL/COBALT EFFLUX SYSTEM RCNA

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GKJ6
AlphaFold — — full sequence — Viewing
ColabFold KP13_03652
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
24 0.912
18 0.674

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 7.37 0.386
2 6.61 0.335
3 6.53 0.331
4 5.69 0.275
5 2.03 0.044