Protein profile

KP13_20435

7-cyano-7-deazaguanine synthase

Genome: KpKP13

Gene: queC ANJ86622.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GNL1
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Amino acids 233
Annotations 5
Features 16
PDB binders 0
Druggability 0.539

Overview

Basic information about this protein and its source genome.

Accession
KP13_20435
Assembly
Klebsiella pneumoniae subsp. pneumoniae Kp13, complete sequence
Gene
queC ANJ86622.1
Status
annotated
Amino acids
233
Structure source
AlphaFold + ColabFold
EC
6.3.4.20
GO
GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0016879 Catalysis of the joining of two molecules, or two groups within a single molecule, via a carbon-nitrogen bond, with the concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate. GO:0008270 Binding to a zinc ion (Zn). GO:0008616 The chemical reactions and pathways resulting in the formation of queuosines, a series of nucleosides found in position 34 of tRNA and having an additional pentenyl ring added via an NH group to the methyl group of 7-methylguanosine. The pentenyl ring may carry other substituents. The wobble nucleoside of the tRNA sequence (position 34) corresponds to the first position of the anticodon.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
0.0
Localization
Unknown
ColabFold pLDDT
89.64

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.539
Structure A0A0H3GNL1
Pocket Pocket 15
P2Rank 0.895
Structure A0A0H3GNL1
Pocket Pocket 1
ColabFold model
FPocket 0.764 · Pocket 2
P2Rank 0.941 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 464 / 4744 genomes with a hit
Normalized 0.098

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 4 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

4
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0016879 Catalysis of the joining of two molecules, or two groups within a single molecule, via a carbon-nitrogen bond, with the concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate.
  • GO:0008270 Binding to a zinc ion (Zn).
  • GO:0008616 The chemical reactions and pathways resulting in the formation of queuosines, a series of nucleosides found in position 34 of tRNA and having an additional pentenyl ring added via an NH group to the methyl group of 7-methylguanosine. The pentenyl ring may carry other substituents. The wobble nucleoside of the tRNA sequence (position 34) corresponds to the first position of the anticodon.

Sequence Features

Domain/signature hits from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
3 212 CDD cd01995 ExsB
3 212 InterPro IPR018317 Queuosine biosynthesis protein QueC
4 206 NCBIfam TIGR00364 7-cyano-7-deazaguanine synthase QueC
4 206 InterPro IPR018317 Queuosine biosynthesis protein QueC
1 225 PIRSF PIRSF006293 ExsB
1 225 InterPro IPR018317 Queuosine biosynthesis protein QueC
2 217 PANTHER PTHR42914 7-CYANO-7-DEAZAGUANINE SYNTHASE
2 217 InterPro IPR018317 Queuosine biosynthesis protein QueC
3 213 Pfam PF06508 Queuosine biosynthesis protein QueC
3 213 InterPro IPR018317 Queuosine biosynthesis protein QueC
2 219 Hamap MF_01633 7-cyano-7-deazaguanine synthase [queC].
2 219 InterPro IPR018317 Queuosine biosynthesis protein QueC
1 221 FunFam G3DSA:3.40.50.620:FF:000017 7-cyano-7-deazaguanine synthase
1 214 SUPERFAMILY SSF52402 Adenine nucleotide alpha hydrolases-like
1 231 Gene3D G3DSA:3.40.50.620 HUPs
1 231 InterPro IPR014729 Rossmann-like alpha/beta/alpha sandwich fold

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GNL1
AlphaFold — — full sequence — Viewing
ColabFold KP13_20435
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
15 0.539

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 17.85 0.809
2 2.39 0.063